BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0034.p (459 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 230 2e-61 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 218 1e-57 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 204 1e-53 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 129 6e-31 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 120 2e-28 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 230 bits (586), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 152/466 (32%), Positives = 254/466 (54%), Gaps = 19/466 (4%) Query: 6 SLTVRLTALFALASTAVLVGLGLLIISTVAKHFTDQDEQLLEKELQLIRMVVNERGADAI 65 SL++RL ALF L + +L LG + ++ +D++ L + + I +V A ++ Sbjct: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEH--AASL 65 Query: 66 TSTFG------EALHNHPGFYVHISSLSGQALYSTLNHA-TAPILAAAQSAVPGRSFAVP 118 + L H V I+ G L + + T P + +Q + A Sbjct: 66 EDILARRHLVLDTLTGHDQLLVRIARADGSTLLESHDPTRTPPGIDLSQVDIR----AWD 121 Query: 119 VGQHQKFQAIKARL---KDDQSARELQVIVAVDTEIHDHFMRNFQTTLVLYIAG-SVLIV 174 Q K ++AR K + E V++ V T + + V + AG L+ Sbjct: 122 SIQSWKLGDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALLA 181 Query: 175 VLLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMSVEMLPIEIADLAAKLNAMLERLQRD 234 +L+S++ R GL PLR M+ + +AVS++ R+ ++ P E+ ++ N ML+RL R Sbjct: 182 ILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRS 241 Query: 235 FNRITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDILSSNAEELQRLARTISDM 294 F +++ FS D+AH++RTP+ NL+ QT V L + R + Y+ +LSSN EE +RL+R + M Sbjct: 242 FQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESM 301 Query: 295 LFLAQTENGITLPSYERLSLQEEISELFDFYDALAEEKNVLMRLAGEASIRGDRLMVRRA 354 LFLA+ EN + ++ L + +E++ + D+++ +A+E V + + G ++ D ++RRA Sbjct: 302 LFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRRA 361 Query: 355 LSNLISNAMRYTPPAGVIAVSIDRSEKDISVHVENDGPEIPSEHLPHLFDRFYRADKART 414 + NL++NA+RYTP GVI + +D + + + V N G IP+E L LFDRFYRAD +R+ Sbjct: 362 VGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSRS 421 Query: 415 KLDSDSAGLGLSITKAIMRAHQGEVSVRSAQGK-TRFTLLFPNAAA 459 S LGL+I ++IM H G+ SV S+ G+ T F+L FP +A Sbjct: 422 SAGGSSG-LGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFPGRSA 466 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 218 bits (554), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 139/453 (30%), Positives = 233/453 (51%), Gaps = 15/453 (3%) Query: 4 QRSLTVRLTALFALASTAVLVGLGLLIISTVAKHFTDQDEQLLEKELQLIRMVVNERG-- 61 ++S+T RL + A ++ + G+++ + + + L L I + G Sbjct: 9 RQSITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISYQIGRAGNT 68 Query: 62 -----ADAITSTFGEALHNHPGFYVHISSLSGQALYSTLNHATAPILAAAQSAVPGRSFA 116 A +T +A H F+ ISS + Y L+ + A + GR Sbjct: 69 ERWGRVQAKLATLSQA-DQHVSFW--ISSSDARFTYGDLSRLAPQAIRADEGNSVGR-LH 124 Query: 117 VPVGQHQKFQAIKARLKDDQSARELQVIVAVDTEIHDHFMRNFQTTLVLYIAGSVLIVVL 176 +P G+ + L + + V V VD E + R F ++ +++ V+ Sbjct: 125 LP-GRRYPLHIMTRTLPAFEERPAVDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVLAVI 183 Query: 177 LSWWAARRGLAPLRAMSDKVQAVSSHNFGERMSVEMLPIEIADLAAKLNAMLERLQRDFN 236 S+W AR GLAPLR MSD+ Q+ S RM VE +P E++ LA N L+R++ + Sbjct: 184 SSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIEDAYT 243 Query: 237 RITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDILSSNAEELQRLARTISDMLF 296 ++ F+ D+AHELRTP+ NL+ +T V L ++R+ ++D+L SN EEL R+ ++DMLF Sbjct: 244 QLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLF 303 Query: 297 LAQTENGITLPSYERLSLQEEISELFDFYDALAEEKNVLMRLAG--EASIRGDRLMVRRA 354 LA+++ G + + +E+ + DF++ + ++ + + +AG EA+ + + +RA Sbjct: 304 LARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMMETALFKRA 363 Query: 355 LSNLISNAMRYTPPAGVIAVSIDRSEKDISVHVENDGPEIPSEHLPHLFDRFYRADKART 414 L+NLI NA++++P ++ + I E+ + V V N G I HLP LFDRFYR D AR Sbjct: 364 LTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDSARQ 423 Query: 415 KLD-SDSAGLGLSITKAIMRAHQGEVSVRSAQG 446 ++ S GLGL+I KA+ R H G S +G Sbjct: 424 DINGSHGHGLGLAIVKAVARMHGGRAFASSKEG 456 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 204 bits (520), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 146/466 (31%), Positives = 244/466 (52%), Gaps = 17/466 (3%) Query: 6 SLTVRLTALFALASTAVLVGLGLLIISTVAKHFTDQDEQLLEKELQLIRMVV----NERG 61 +LT R+TALFAL ++ V+ GLG + + + + + +Q L ++ R ++ N Sbjct: 12 TLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHDLYNVEQ 71 Query: 62 ADAITSTFGEALHNHPGFYVHISSLSGQALY--STLNHATAPILAAAQSAVPGRSFAVPV 119 + + F + N V I G+A + S +H P + P S ++ Sbjct: 72 MEQRPALFESMMGNEQD--VRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGSPVSSASLRT 129 Query: 120 GQHQKFQAIKAR----LKDDQSARELQVIVAVDTEIHD-HFMRNFQTTLVLYIAGSVLIV 174 Q+ ++ R L D + IVA + + MR+++ + A +V++ Sbjct: 130 --SQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAAAAAVVLT 187 Query: 175 VLLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMSVEMLPIEIADLAAKLNAMLERLQRD 234 LL + RRGL PL AMS + ++ + R+ E +P E+ +A NAML+RL+ Sbjct: 188 ALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAG 247 Query: 235 FNRITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDILSSNAEELQRLARTISDM 294 + + FS D+AHE+RTPI L+ Q+ V L Q+R+ E Y +L SN EELQRL+R + ++ Sbjct: 248 YAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVENI 307 Query: 295 LFLAQTENGITLPSYERLSLQEEISELFDFYDALAEEKNVLMRLAGEASIRGDRLMVRRA 354 LFLAQ ++ L L EE+ + D+++ +A+E+ + + + + +M RR Sbjct: 308 LFLAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIMWRRV 367 Query: 355 LSNLISNAMRYTPPAGVIAVSIDRSEKDISVHVENDGPEIPSEHLPHLFDRFYRADKART 414 +SNL+ NA+RY I + + + + VEN ++ ++ + +FDRFYR D++R+ Sbjct: 368 VSNLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDRSRS 427 Query: 415 KLDSDSAGLGLSITKAIMRAHQGEVSVRS-AQGKTRFTLLFPNAAA 459 ++S GLGL+I KAIM H G +V A G RF+L FP+AAA Sbjct: 428 AF-TESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRFPSAAA 472 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 129 bits (323), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 90/292 (30%), Positives = 148/292 (50%), Gaps = 14/292 (4%) Query: 170 SVLIVVLLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMSVEMLPIEIADLAAKLNAMLE 229 S+ +V++ A RRGL PL+++ ++ G + P+E+ L +N ++ Sbjct: 174 SIFVVLV----AVRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIVAMNELMA 228 Query: 230 RLQRDFNRITNFSTDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDILSSNAEELQRLAR 289 R+ + NF D+AH+LRTP+T L Q +++ + + L+ ++R+ R Sbjct: 229 RINKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIR 288 Query: 290 TISDMLFLAQTENGI-TLPSYERLSLQEEISELFDFYDALAEEKNVLMRLA-GEASIRGD 347 +L LA++E G+ E LSL + + E + A++K + + A +RGD Sbjct: 289 QSRQLLALARSEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLRPARLRGD 348 Query: 348 RLMVRRALSNLISNAMRYTPPAGVIAVSIDRSEKDISVHVENDGPEIPSEHLPHLFDRFY 407 R ++ + NLI NA+RY+PP G + V + + VE+ GP I EH +FDRFY Sbjct: 349 RFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFY 408 Query: 408 RADKARTKLDSDSAGLGLSITKAIMRAHQGEVSVRSA-QGK-TRFTLLFPNA 457 RA+ +GLGL+I + I H G +SV QG+ T FT+ FP A Sbjct: 409 RANDKVA-----GSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLA 455 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 120 bits (301), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 91/295 (30%), Positives = 147/295 (49%), Gaps = 20/295 (6%) Query: 174 VVLLSWWAARRGLAPLRAMSDKVQAVSSHNFGERMSVEMLPIEIADLAAKLNAMLERLQR 233 + +LSW A RGLAP+R + +V A ++ V M P EI L LN +L RL+ Sbjct: 153 IGVLSWVAVSRGLAPVRKAAAEVAARDMNSLQPLADVGM-PQEIQPLTHALNDLLARLKH 211 Query: 234 DFNRITNFSTDIAHELRTPITNLLTQTDV---VLTQQRTNEAYRDILSSNAEELQRLART 290 F D AHELRTP+T L QT + + ++ A+ D+ + L+R Sbjct: 212 ATETQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFADLKNG----LERTTHL 267 Query: 291 ISDMLFLAQTENGITLPSYERLSLQEEISELFDFYDALAEEKNVLMRLAGEAS------I 344 + +L LA+ E S+ ++ L+ + + ++AE + + L+ + + Sbjct: 268 VHQLLTLARQEPDAM--SFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRV 325 Query: 345 RGDRLMVRRALSNLISNAMRYTPPAGVIAVSIDRSEKD-ISVHVENDGPEIPSEHLPHLF 403 G+ +R +NL+ NA+RYTP GV+ V +D S +V V++ GP I LP +F Sbjct: 326 TGNADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVF 385 Query: 404 DRFYRADKARTKLDSDSA---GLGLSITKAIMRAHQGEVSVRSAQGKTRFTLLFP 455 DRFYRA ++ S +A GLGL+I + I H+ V +R+ + ++FP Sbjct: 386 DRFYRAQHSQEGSGSRAAFGSGLGLAIVRQIADLHRVPVELRNTGHGLQARVVFP 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,231,488 Number of extensions: 47598 Number of successful extensions: 342 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 37 Length of query: 459 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 371 Effective length of database: 1,203,190 Effective search space: 446383490 Effective search space used: 446383490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)