BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0032.p (438 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 555 e-159 gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 170 2e-43 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 133 3e-32 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 103 4e-23 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 555 bits (1429), Expect = e-159, Method: Compositional matrix adjust. Identities = 309/370 (83%), Positives = 333/370 (90%) Query: 1 MEQTLLHPLIYLLAAVVAVPIAKKLGLGAVLGYLLAGIAIGPWGLGLISEVEDILHFSEF 60 M++ LL L+YLLAAV AVPIAK+LGLGAVLGYLLAGI IGPWGLGLI+EV+ IL FSEF Sbjct: 1 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF 60 Query: 61 GVVLLLFLIGLELDPKRLWSLRRHIFGWGSAQVGLVSLGLFGAALAVGIDWRVALIGALG 120 GVVLLLFLIGLELDPKRLW+LRR IFGWGS QV VSL L AALA + WR+ LIGALG Sbjct: 61 GVVLLLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAALAASVPWRIGLIGALG 120 Query: 121 MSLSSTAIALATLGERKLTATPAGSAGFAILLFQDIAAIPMMAAVPLLGVAVAQGSGQGW 180 MSLSSTAIALATLGERKLTATPAGSA F+ILLFQDIAAIPMMAAVPLLGVA +QGSGQGW Sbjct: 121 MSLSSTAIALATLGERKLTATPAGSAAFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGW 180 Query: 181 LHTLQVVAVIALLIFGGRYLIRPILRIIAKTDMREIFTAFSLLLVIATCLLMQSVGMSMA 240 LH QV VIALLIFGGR L+RPILRIIAKTDMREIFTAF+LLLVIATCLLMQSVGMSMA Sbjct: 181 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMA 240 Query: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGIFLEQPWLILGMVLG 300 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFG+F QPW ILGMVLG Sbjct: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLG 300 Query: 301 FLVIKTAVLYGLAKCFRIPRDQQIFFSFLLSQGGEFAFVVFGAAATAQVLTPRISSILVV 360 FL IKT V++ L +CF IPR QQ+FF+FLLSQGGEFAF+VFGAA TA+VLTP SS+LVV Sbjct: 301 FLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVV 360 Query: 361 VVALSMVITP 370 VVALSM++TP Sbjct: 361 VVALSMLVTP 370 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 170 bits (431), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 132/388 (34%), Positives = 213/388 (54%), Gaps = 20/388 (5%) Query: 9 LIYLLAAVVAVPIAKKLGLGAVLGYLLAGIAIGPWGLGLISEVEDILHFSEFGVVLLLFL 68 L L AAV+ V + + L +LGYL+ GI IGP GLG + E EFGVV L+F Sbjct: 9 LFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLGFAEDNETTHALGEFGVVFLMFS 68 Query: 69 IGLELDPKRLWSLRRHIFGWGSAQVGL-----VSLGLFGAALAV----GIDWRVALIGAL 119 IGLE +L ++R +FG G AQV + LG +GAA + I W+ A Sbjct: 69 IGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLG-WGAATFLPQFFNISWQAAFALGG 127 Query: 120 GMSLSSTAIALATLGERKLTATPAGSAGFAILLFQDIAAIPMMAAVPLLGVAVAQGSGQ- 178 +++SSTAI L ER T G +LLFQD+A +P++ VP A+A SG Sbjct: 128 ALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVP----ALANESGNI 183 Query: 179 ----GWLHTLQVVAVIALLIFGGRYLIRPILRIIAKTDMREIFTAFSLLLVIATCLLMQS 234 GW +V + LL FG + L+R RI+ K +E+F LL+ + + + Sbjct: 184 MVTLGWAAGKALVVLALLLFFGSK-LMRGWFRIVVKRRSQELFMLNLLLITLGAAWITEK 242 Query: 235 VGMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGIFLEQPWLI 294 G+S+ALG F+AG+L++++E++H +E D++ F+ +LLGLFFI +GM ++ ++ +L+ Sbjct: 243 AGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHWFLV 302 Query: 295 LGMVLGFLVIKTAVLYGLAKCFRIPRDQQIFFSFLLSQGGEFAFVVFGAAATAQVLTPRI 354 L ++ G +++K ++ LA+ F + L+Q GEF FV+ A +++ P + Sbjct: 303 LLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDPML 362 Query: 355 SSILVVVVALSMVITPLLLLFHDRVLLP 382 +++ + LSM+ TP +L D +++ Sbjct: 363 IQVILAAMVLSMLATPFILAKSDAIVMK 390 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 133 bits (334), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 122/379 (32%), Positives = 193/379 (50%), Gaps = 14/379 (3%) Query: 21 IAKKLGLGAVLGYLLAGIAIGPWGLGLISEVEDILHFSEFGVVLLLFLIGLELDPKRLWS 80 +A +L + ++GYL+AGIAIGP+ G + + + +E GV+LL+F +GL K L S Sbjct: 24 LAHRLKMPPLVGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLS 83 Query: 81 LRRHIFGWGSAQVGLVSLGLFGAALAVGIDWRVA--LIGALGMSLSSTAIALATLGERKL 138 ++ AQ+ + +L G AL + W V + L +S++ST + L L ER+L Sbjct: 84 VKNIAIPGAVAQITIATL--LGMALGWLLGWPVGEGFLFGLALSVASTVVLLKALQERRL 141 Query: 139 TATPAGSAGFAILLFQDIAAIPMMAAVPLL-GVAVAQG---SGQGWLHTL-----QVVAV 189 T G L+ +DIA + + +P L G+ +G SG L TL +VVA Sbjct: 142 VETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGNAVLLTLGITLGKVVAF 201 Query: 190 IALLIFGGRYLIRPILRIIAKTDMREIFTAFSLLLVIATCLLMQSV-GMSMALGTFLAGV 248 A+++ GR +I IL IA T RE+F L + + L V G+S ALG F AG+ Sbjct: 202 AAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSFALGAFFAGM 261 Query: 249 LLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGIFLEQPWLILGMVLGFLVIKTAV 308 +L++SE H + P + LFF++VGM D I +P L+L VL +V K+ Sbjct: 262 ILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVAREPLLVLATVLIIVVGKSLG 321 Query: 309 LYGLAKCFRIPRDQQIFFSFLLSQGGEFAFVVFGAAATAQVLTPRISSILVVVVALSMVI 368 + + F P + S L+Q GEF+F++ + +L+P +++ LS+++ Sbjct: 322 ALLIVRAFGYPNGTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILGGAILSILL 381 Query: 369 TPLLLLFHDRVLLPRPPPP 387 PLL DR +P P Sbjct: 382 NPLLFSLLDRYEARQPKAP 400 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 103 bits (256), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 105/385 (27%), Positives = 189/385 (49%), Gaps = 31/385 (8%) Query: 9 LIYLLAAVVAVPIAKKLGLGAVLGYLLAGIAIGPWG--LGLISEVEDILHFSEFGVVLLL 66 +I L+A +V V + KL VLGY++AG+ IGP LI + + + SE GV+ LL Sbjct: 13 IIMLIAGIVTV-VFNKLKQPVVLGYIVAGVIIGPHTPPYDLIQDEKTVHILSELGVIFLL 71 Query: 67 FLIGLELDPKRLWSLRRHIFGWGSAQVGLVSLGLFGAALAVGIDWR---VALIGALGMSL 123 F +GLE K+L + F +A++ L+ G + + W+ +GA+ +++ Sbjct: 72 FSLGLEFSLKKLAKVGATAFIGAAAEILLMIW--IGYEIGLYFGWKRMDAIFLGAM-LAV 128 Query: 124 SSTAIALATLGERKLTATPAGSAGFAILLFQDIAAIPMMAAVPLLGVAVAQGSGQGWLHT 183 SST I + L E + F IL+ +DI AI M+A + + + + S + + Sbjct: 129 SSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVATSGSVDSTEVFTTV 188 Query: 184 LQVVAVIALLIFGGRYLIRPILRIIAKTDMREIFTAFSLLLVIATCLLMQSVGMSMALGT 243 ++V + + + G + +L I++ E+ L ++ CLL+ + S+ALG Sbjct: 189 GKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLVMKLQYSVALGA 248 Query: 244 FLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGIFLEQPWLILGMVLGFLV 303 FL G ++A+S + H +E +EP + + +FF+A+G+ D P +++ + V Sbjct: 249 FLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFD-------PNVLVKYWMPITV 301 Query: 304 IKTAVLYG-LAKC----------FRIPRDQQIFFSFLLSQGGEFAFVVFGAAATAQVLTP 352 I AV++G L C R P L+Q GEF+F++ + +V + Sbjct: 302 ITLAVVFGKLISCGLGTFIAGQGGRTPMR----VGMGLAQIGEFSFIIAALGQSLKVTSD 357 Query: 353 RISSILVVVVALSMVITPLLLLFHD 377 + I+V V A++ ++TP L+ D Sbjct: 358 FLYPIVVAVSAVTALLTPYLIKAAD 382 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.144 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,097,694 Number of extensions: 44283 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 260 Number of HSP's successfully gapped: 4 Length of query: 438 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 350 Effective length of database: 1,203,190 Effective search space: 421116500 Effective search space used: 421116500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)