BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0003.0095.p
         (297 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance ...   434   e-123
gi|300313899|ref|YP_003777991.1| RND family efflux transporter M...    75   5e-15

>gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance
           cation efflux system transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 396

 Score =  434 bits (1117), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 212/297 (71%), Positives = 255/297 (85%)

Query: 1   VVPKVNGVVESVSANLGQQVKKGQVLAVVSSSTVSEQRSELLNAQQRLTLAQSTHAREKT 60
           VVP+V G+VESVSA LGQ+V+KGQVLAV++SS VSEQRSE+LNA+QRL LA++ + REK 
Sbjct: 100 VVPQVAGIVESVSAKLGQKVRKGQVLAVINSSAVSEQRSEMLNAEQRLALARTAYQREKQ 159

Query: 61  LWQEKISAEQDYLQAGQALKEAQIAVRNAQQKLAAIGAGAASSGLNRYEIRAPFDGIVVE 120
           LW++KISAEQDYLQA  AL+EA++AVRNAQQKL AIGA A+S GLNRYEIRAPFDG+VVE
Sbjct: 160 LWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRAIGATASSGGLNRYEIRAPFDGVVVE 219

Query: 121 KHLALGEAVKEDAGIFIISDLSSVWAEITVAARDMSAVRVGESVTVNATAFASSASGKIA 180
           KHL LGEAVKEDA  F+ISDLSSVWAEI V+ +DM  VR GE VTV ATAF +SASGKI+
Sbjct: 220 KHLGLGEAVKEDANAFLISDLSSVWAEIIVSPKDMQFVRTGEKVTVRATAFDASASGKIS 279

Query: 181 YVGALLGEQTRTAKAHVVLPNPGNVWRPGLFVNVDVLAQETDVALAVAADAVQSVEQKQV 240
           YVG+L+GEQ+RTAKAHV+LPNP + WRPGLFVNV+V++ E  V +AV+A+A+Q+VE+K V
Sbjct: 280 YVGSLIGEQSRTAKAHVILPNPDDSWRPGLFVNVEVVSDEAQVPVAVSAEALQTVEEKSV 339

Query: 241 VFVRVADGFRKQEVTLGRTDGKRVEILKGLSAGDVYAAAGSFVIKAELGKGSAEHAH 297
           +FVRV  GF  Q+V  GR++GK VEI  GL+AGD YAA GSF IKAELGKGSAEH+H
Sbjct: 340 IFVRVPGGFAAQQVVTGRSNGKFVEIRSGLNAGDEYAATGSFAIKAELGKGSAEHSH 396


>gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP
           subunit [Herbaspirillum seropedicae SmR1]
          Length = 406

 Score = 75.5 bits (184), Expect = 5e-15,   Method: Compositional matrix adjust.
 Identities = 75/278 (26%), Positives = 136/278 (48%), Gaps = 15/278 (5%)

Query: 7   GVVESVSANLGQQVKKGQVLAVVSSSTVSEQRSELLNAQQR----LTLAQSTHAREKTLW 62
           G ++  + ++G +VKKG++LA + +  + +Q ++ L A+++    + LA+S+ AR + L 
Sbjct: 104 GYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQALAAREQAASSMELARSSMARWEALR 163

Query: 63  QEKISAEQDYLQAGQALKEAQIAVRNAQQKLAAIGAGAASSGLNRYEIRAPFDGIVVEKH 122
           ++   ++Q+  +   A  +A   +  A      +G      G  R  I APF GI+  ++
Sbjct: 164 KKDAVSQQELDERRSAFAQASANLAAADAN---VGRLRQVEGFKR--IVAPFSGIITRRN 218

Query: 123 LALGEAVKEDAG--IFIISDLSSVWAEITVAARDMSAVRVGESVTVNATAF-ASSASGKI 179
           + +G+ +   AG  +F++S    +   + V     + V+ G+ VTV  +        GK+
Sbjct: 219 VDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQSYANLVKPGQQVTVTQSELRGRKFEGKV 278

Query: 180 AYVGALLGEQTRTAKAHVVLPNPGNVWRPGLFVNVDVLAQETDVALAVAADAVQSVEQKQ 239
           A     +   TR+ +  V L N  +   PG FV V  L  +   AL VAAD +       
Sbjct: 279 ARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQV-ALPIKPSGALLVAADTLMFRRDGT 337

Query: 240 VVF-VRVADGFRKQEVTLGRTDGKRVEILKGLSAGDVY 276
           +V  V   +  R +++ LGR  G+ VE+L GL  GD +
Sbjct: 338 LVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGLQ-GDEH 374


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.313    0.127    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 760,859
Number of extensions: 25774
Number of successful extensions: 125
Number of sequences better than 1.0e-08: 10
Number of HSP's gapped: 121
Number of HSP's successfully gapped: 10
Length of query: 297
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 212
Effective length of database: 1,217,395
Effective search space: 258087740
Effective search space used: 258087740
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 130 (54.7 bits)