BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0003.0094.p
         (419 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313718|ref|YP_003777810.1| cobalt-zinc-cadmium resistance ...   426   e-120
gi|300310931|ref|YP_003775023.1| copper tolerance protein [Herba...    56   6e-09

>gi|300313718|ref|YP_003777810.1| cobalt-zinc-cadmium resistance
           cation efflux system outer membrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 428

 Score =  426 bits (1094), Expect = e-120,   Method: Compositional matrix adjust.
 Identities = 219/393 (55%), Positives = 296/393 (75%), Gaps = 6/393 (1%)

Query: 26  ATSPTLNITLNNAVALALDNNPELSAARHELEAVDATVTQAGARPNPELSTLMEDTRKST 85
           A+ P L ++L  A++LAL+ N  L+AAR E+EAV+AT+ QAGARPNPE S LMED RKS+
Sbjct: 40  ASEPRL-LSLPAAISLALEQNKTLAAARKEIEAVEATIIQAGARPNPEFSALMEDFRKSS 98

Query: 86  RTTTIQLNQPIELGGKRAARIGAPRGXPAERSREAARMELAIRRDDIRIAATSAFFAVLT 145
           RTTT QL QPIELGGKR+ARI A     A+R+RE A ++ A +R +IR    +A++ V+ 
Sbjct: 99  RTTTYQLTQPIELGGKRSARIEA-----AQRTREMALLDYAAKRTEIRAGVIAAYYEVMV 153

Query: 146 AQERRRLAQESAELARSAADATGRRVTAGKISPVEETRARVAAASARLELGQAEGELDLA 205
           A ER+RLA+E   LA+++AD T RRVTAGKISPVE+T+ARVA +SA+LEL QA+ ++ LA
Sbjct: 154 AMERQRLAKELLGLAKTSADMTSRRVTAGKISPVEQTKARVAESSAQLELHQADSDVLLA 213

Query: 206 RRRLAGLWGKAQPRFTLAEGVAGQLPPLPAIADIAERSTDAPQLRLARLEVERRQALSKI 265
           +++LA  WG     F L +G    LP LP+IA + E+    P ++LA+LEVERR+AL++I
Sbjct: 214 QKKLATFWGSVGNAFQLTDGGVESLPALPSIAQLTEKVAAGPAVQLAQLEVERRRALARI 273

Query: 266 ESSKRIPDATLSLGRKRDEEAGRSMWVVGVSMPLPFLDSNRGNLEEALRRTDKARDELAA 325
           E+SKR PD TLS G KRDE   R+MWVVG S+P+P LDSN+GN  EAL+R D ARDEL+ 
Sbjct: 274 ETSKRTPDVTLSFGNKRDESNARNMWVVGFSVPIPVLDSNKGNELEALKRVDIARDELSI 333

Query: 326 AETRVRTDIAQAYEKLRNAHAEVAVLQSDILPGAQSAYAAAGKGFELGKFNFLEVLDAQR 385
            E +++++ AQ++E+LRNA  E   L+++I+PGAQSAY AA  GFELGKF +L+VLDAQR
Sbjct: 334 VEVQMQSEAAQSFERLRNAREEAVALRNEIVPGAQSAYQAARTGFELGKFAYLDVLDAQR 393

Query: 386 TLFQARSQYLRAISDAHLASAELERLLGSAGPN 418
           T FQA++QY +A+S A  A+A+L+RL G+  P+
Sbjct: 394 TYFQAKAQYWKALSTAFQAAADLDRLAGNETPS 426


>gi|300310931|ref|YP_003775023.1| copper tolerance protein
           [Herbaspirillum seropedicae SmR1]
          Length = 461

 Score = 55.8 bits (133), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 90/332 (27%), Positives = 147/332 (44%), Gaps = 26/332 (7%)

Query: 36  NNAVALALDNNPELSAARHELEAVDATVTQAGARPNPELSTLMEDTRKSTRTTTIQLNQP 95
           ++AV +AL NNP+L A    L   +A + QAG  PNP  S      ++S     +++ + 
Sbjct: 81  DDAVQIALINNPDLQADFAGLGVAEADLVQAGRLPNPGFS-----FKRSHAGDDLKIERS 135

Query: 96  IELGGKRAARIGAPRGXPAERSR-EAARMELAIRRDDIRIAATSAFFAVLTAQERRRLAQ 154
           + LG  R   +  P     E+ R E  R+ LA R   +      A++  + AQ+  +  +
Sbjct: 136 LSLGLMRL--LTLPAASRIEQRRFEQVRLSLASRVLSVAAQTRQAYYQAVAAQQGLQYQK 193

Query: 155 ESAELARSAADATGRRVTAGKISPVEETRARVAAASARLELGQAEGELDLARRRLAGLWG 214
           + A+ A +A     +    G IS ++  R +     A+  L QA       +  LA L G
Sbjct: 194 QVADAAEAAHALAAQMARLGNISQLDSAREQFFYGQAQTSLQQARRLAAQHKESLARLLG 253

Query: 215 KAQPRFTLAEGVAGQLPPLP----AIADIAERSTDAPQLRL----ARLEVERRQALSKIE 266
            A P F+L      QLP LP    A+ DI ++   A Q RL    AR E+E  Q    + 
Sbjct: 254 LA-PDFSL----PAQLPALPRQLEAVDDIEQQ---ALQQRLDVEAARTELEGLQTSLGLT 305

Query: 267 SSKRIPDATLSLGRKRDEEAGRSMWV-VGVSMPLPFLDSNRGNLEEALRRTDKARDELAA 325
            + R+ +  L LG  R  E+G+ +     +S+ +P  D     + +A     +   +LAA
Sbjct: 306 RATRLVN-VLDLGALRTTESGKPVENGYEISLEIPLFDWGEARVAKAEAIYLQGAHKLAA 364

Query: 326 AETRVRTDIAQAYEKLRNAHAEVAVLQSDILP 357
           A    R     A+ + ++A+      Q + LP
Sbjct: 365 AVLDARAQARLAWRERQDAYTLARRYQDEWLP 396


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.129    0.350 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,077,666
Number of extensions: 41279
Number of successful extensions: 248
Number of sequences better than 1.0e-08: 7
Number of HSP's gapped: 243
Number of HSP's successfully gapped: 7
Length of query: 419
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 331
Effective length of database: 1,203,190
Effective search space: 398255890
Effective search space used: 398255890
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 132 (55.5 bits)