BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0090.p (382 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas... 710 0.0 gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 81 2e-16 gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 77 2e-15 gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 65 8e-12 gi|300310379|ref|YP_003774471.1| hypothetical protein Hsero_1046... 60 3e-10 >gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 710 bits (1833), Expect = 0.0, Method: Compositional matrix adjust. Identities = 351/382 (91%), Positives = 367/382 (96%) Query: 1 MKITKLTTFIVPPRWCFLKIETDEGIVGWGEPVVEGRAHSVAAAVEELADYLIGKDPRNI 60 MKITK+TTFIVPPRWCFLKIETDEG+VGWGEP+VEGRAHSVAAAVEEL+DYLIGKDPRNI Sbjct: 1 MKITKITTFIVPPRWCFLKIETDEGVVGWGEPIVEGRAHSVAAAVEELSDYLIGKDPRNI 60 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRVYSWI 120 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGK LG+PV+QLLGG VR+SIRVYSWI Sbjct: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRVYSWI 120 Query: 121 GGDRPADTAKAAKDAVARGFTAVKMNGTEELQFIDTYDKVELTLANVAAVREAVGPNVGI 180 GGDRPADTA AAK AVARGFTAVKMNGTEELQFID++DK+ELTLANV AVREAVGPNVGI Sbjct: 121 GGDRPADTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI 180 Query: 181 GVDFHGRVHKPMAKVLVKELEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLYSR 240 GVDFHGRVHKPMAK L+KELE YKLMFIEEPVLSEN EALKEIAHLT TPIALGERLYSR Sbjct: 181 GVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLYSR 240 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYDVALALHCPLGPIALAACLQVD 300 WDFKRILSEGYVDIIQPDVSHAGGITETRKIA MAEAYDVALALHCPLGPIALA+CLQVD Sbjct: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD 300 Query: 301 AVSYNAFIQEQSLGIHYNESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEYVRQR 360 A SYNAFIQEQSLGIHYNESNDLLDY+ D +VF+Y+DGYV IP GPGLGIEINEEYVRQR Sbjct: 301 AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR 360 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 AEVGHRWRNPIWRHKDGSFAEW Sbjct: 361 AEVGHRWRNPIWRHKDGSFAEW 382 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 83/355 (23%), Positives = 151/355 (42%), Gaps = 35/355 (9%) Query: 16 CFLKIETDEGIVGWGEPVV-------EGRAHSVAAAVEE-LADYLIGKDPRNIE---DHW 64 ++I ++G+ GWGE E S+ ++ + L+G D + H Sbjct: 39 VLVRITGNDGVAGWGEATTIGGLAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHI 98 Query: 65 TVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRV-YSWIGGD 123 +G + A I+ AL D + LG+P++ L GG VRDS+ + ++ GD Sbjct: 99 DEHVQGNRF--------AKCAIETALSDALARRLGVPLSTLFGGRVRDSLPIAWTLASGD 150 Query: 124 RPADTAKAAKDAVARGFTAVKMNGTEELQFIDTYDKVELTLANVAAVREAVGPNVGIGVD 183 D +A K R K+ V+ A+VAA++ A+G + VD Sbjct: 151 TARDIDEAEKMLAQRRHNLFKLKIGAR--------TVQQDCAHVAAIKRALGERGSVRVD 202 Query: 184 FHGRVHKPMAKVLVKELEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLYSRWDF 243 + + A + ++ L + +E+P+ + N++ L+ + I E L+ D Sbjct: 203 VNQAWTETEAMLGMQWLADAGVDLVEQPIAASNHDGLRRLRQKNRIAIMADESLHGPQDA 262 Query: 244 KRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYDVALALHCPL-GPIALAACLQVDAV 302 ++ G D+ ++ +GG+ +++A +A A V L L G I A Q+ A Sbjct: 263 YQLARAGAADVFAVKINQSGGLAGAQQVAAIASAAQVELYGGTMLEGAIGTMASAQLFA- 321 Query: 303 SYNAFIQEQSLGIHYNESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEYV 357 ++ L + ++L A Y+D + +P GPGLGI ++E+ V Sbjct: 322 TFGTLAWGSELFGPLLLTEEIL-----ATPLRYRDFALELPTGPGLGITLDEDRV 371 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 97/401 (24%), Positives = 176/401 (43%), Gaps = 59/401 (14%) Query: 17 FLKIETDEGIVGWGEPVVEGRAHSVAAAVEELADYLIGKDPRNIEDHWTVLYRG------ 70 ++IETD G +G GE + A + V+E L+G DP N+ + + RG Sbjct: 33 IIEIETDNGYIGLGESYGDAPALKILDLVKE---RLVGLDPFNL-NGLRAIVRGVVAAMA 88 Query: 71 -----------GFYRGGAIHMSALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRVYSW 119 G + A+ +A + + A D + + L +P+ LLGG VR I ++ Sbjct: 89 PASNAGAELAPGSHASKAVS-NAYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAY 147 Query: 120 IGG------DRPA-----DTAKAAKDAVARGFTAVKMNGTEELQFIDTYDKVELTLANVA 168 + D P A + + VA+ ++ NG + ++ E +A + Sbjct: 148 LFFKYAQHIDSPYAPDAWGEALSEEQIVAQARKMIEENGFQSIKLKAGALAPEHEVACIK 207 Query: 169 AVREAVGPNVGIGVDFHGRVHKPMAKVLVKELEQYKLMFIEEPVLSENYEALKEIAHLTG 228 A+R+A P+ + +D +G A + + EL L + E+P + E + E+ TG Sbjct: 208 ALRKAF-PDAPLRIDPNGNWSLQTA-IRMAELLGDDLQYYEDP--TPGLEGMAELHRRTG 263 Query: 229 TPIALGERLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYDVALALHCP- 287 P+A + + +R ++ V I+ D + GG+ +T+++A M + + + +++H Sbjct: 264 LPLATNMVVTDFDELRRSVALDSVQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNS 323 Query: 288 -LGPIALAACLQVDAVSYNAFIQEQSLGIHYN-ESNDLLDYVTDADVFSYKDGYVTIPNG 345 LG I+L A V A N + HY + D + V ++G V I + Sbjct: 324 HLG-ISLMAMSHVAAAVENL---SYACDTHYPWQEPD--EEVIQGGKLPIRNGCVQITDA 377 Query: 346 PGLGIEINEEY------------VRQRAEVGH-RWRNPIWR 373 PGLG+E++++ +RQR +VG R P W+ Sbjct: 378 PGLGVEVDQDQLRKLHQLYLECGIRQRDDVGQMRKYQPDWK 418 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 65.1 bits (157), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 76/286 (26%), Positives = 127/286 (44%), Gaps = 32/286 (11%) Query: 82 ALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRV-YSWIGGDRPADTAKAAKDAVARGF 140 A I+ AL+D G+ GLPV+QLLGG R+S+ + ++ GD D +A + R Sbjct: 114 AKCAIETALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRH 173 Query: 141 TAVKMN-GTEELQFIDTYDKVELTLANVAAVREAVGPNVGIGVDFHGRVHKPMAKVLVKE 199 K+ G E V+ +A+VAA+++A+G + VD + + A+ + Sbjct: 174 NIFKLKIGRRE---------VDADVAHVAAIKKALGDRGAVRVDVNMAWSELEAQRGLAG 224 Query: 200 LEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLY---SRWDFKRILSEGYVDIIQ 256 L +E+PV S +AL + I E L S + RI G D+ Sbjct: 225 LVDAGCELVEQPVASP--QALARLKGRHAIAIMADESLTGPASAFALARI---GGADVFA 279 Query: 257 PDVSHAGGITETRKIATMAEAYDVAL----ALHCPLGPIALAACLQVDAVSYNAFIQEQS 312 + +GG+ +++A + +A + L L +G IA A A +E Sbjct: 280 IKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEGAIGTIASAHAF--------ATFRELQ 331 Query: 313 LGIHYNESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEYVR 358 G L + + A+ Y+D + IP+ PGLGI ++EE ++ Sbjct: 332 WGTELFGPLLLTEEIL-AEPLQYQDFQLAIPHRPGLGITLDEERLQ 376 >gi|300310379|ref|YP_003774471.1| hypothetical protein Hsero_1046 [Herbaspirillum seropedicae SmR1] Length = 425 Score = 60.1 bits (144), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 67/266 (25%), Positives = 112/266 (42%), Gaps = 17/266 (6%) Query: 95 GKALGLPVNQLLGGPVRDSIRVYSWIGGDRPADTAKAAKDAVARGFTAVKMNGTEELQFI 154 GKA L V + G P + W+G + A + ++A+ +GF VK+ +L Sbjct: 171 GKAERLRVLEQEGYPCYTT--SAGWLGYE-DAKLRRLCQEAIDQGFNHVKLKVGRDL--- 224 Query: 155 DTYDKVELTLANVAAVREAVGPNVGIGVDFHGRVHKPMAKVLVKELEQYKLMFIEEPVLS 214 DK +T+A RE +GP + +D + A V +L + FIEEP Sbjct: 225 -ADDKRRVTIA-----REVLGPERKLMIDANQVWEVHQAIDWVNQLAFAQPWFIEEPTSP 278 Query: 215 ENYEALKEIAHLTG-TPIALGERLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIAT 273 ++ E ++I G +A GE +R FK+ + +D++Q D GG+ E + Sbjct: 279 DDVEGHRKIREGIGAVKVATGEMCQNRVLFKQFIMRDAIDVVQIDSCRLGGVNEILAVML 338 Query: 274 MAEAYDVALALHCPLGPIALAACLQVDAVSYNAFIQEQSLGIHYNESNDLLDYVTDADVF 333 MA Y + H G + L +Q ++ I G + L ++ D V Sbjct: 339 MAAKYGKVVCPHA--GGVGLCEYVQHLSMIDYLCISGSKEGRVTEYVDHLHEHFVDPCVV 396 Query: 334 SYKDGYVTIPNGPGLGIEINEEYVRQ 359 K+ P+ PG IE+ + + Q Sbjct: 397 --KNAAYMPPSRPGFSIEMKPQSLEQ 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,267,813 Number of extensions: 56037 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 5 Length of query: 382 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 295 Effective length of database: 1,207,925 Effective search space: 356337875 Effective search space used: 356337875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)