BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0075.p (201 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 329 8e-92 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 241 4e-65 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 110 8e-26 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 79 3e-16 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 65 3e-12 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 329 bits (844), Expect = 8e-92, Method: Compositional matrix adjust. Identities = 162/201 (80%), Positives = 175/201 (87%), Gaps = 12/201 (5%) Query: 1 VLKPSAASPELMKHRGKAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEV 60 VLKPSAA+PELMKHRG+AVVFEDF HYKERINDPDLDVDAS VLVMKN GPKGYPGMAEV Sbjct: 397 VLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEV 456 Query: 61 GNMGLPPKLLAQGVKDMVRISDARMSGTAYGTVVLHVAPEAAAGGPLGIVQDGDWIEMDC 120 GNMGLPPK+LA GVKDMVRISDARMSGTAYGTV+LHVAPEAAAGGPLGIVQDGD+IE+D Sbjct: 457 GNMGLPPKVLATGVKDMVRISDARMSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDA 516 Query: 121 AAGRLHLDISDEEMAQRQTARAEKLAQAKPAMQGGYQSLYIDRVLQADEGCDFDFLVGCR 180 AG+L LDIS+EEM +R ARA+ LA+ KP M GGYQSLY+DRVLQADEGCDFDFLVGCR Sbjct: 517 YAGKLQLDISEEEMKRRLEARAKVLAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCR 576 Query: 181 GAXGCDFDFLVGCAAIPKHSH 201 G AA+PKHSH Sbjct: 577 G------------AAVPKHSH 585 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 241 bits (614), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 117/181 (64%), Positives = 148/181 (81%), Gaps = 2/181 (1%) Query: 1 VLKPSAASPELMKHRGKAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEV 60 V+KPSAA+P L+KHRG+AVVFE+ +RI+D +LDVD + VLV+KNCGPKGYPGMAE Sbjct: 392 VIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEA 451 Query: 61 GNMGLPPKLLAQGVKDMVRISDARMSGTAYGTVVLHVAPEAAAGGPLGIVQDGDWIEMDC 120 GNM LPPK+L +G+ DMVR+SDARMSGTAYGTVVLHV+PEAAAGGPL +VQ+GD+IE+D Sbjct: 452 GNMPLPPKILRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGPLAVVQNGDFIELDV 511 Query: 121 AAGRLHLDISDEEMAQRQTARAEKLAQAKPAMQGGYQSLYIDRVLQADEGCDFDFLVGCR 180 A +LHLD+SDEE+A+R+ A+ +K + P MQ G+ LY+D V QA++G D DFLVG Sbjct: 512 EARKLHLDVSDEELARRR-AQWQK-PELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKS 569 Query: 181 G 181 G Sbjct: 570 G 570 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 110 bits (275), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 61/185 (32%), Positives = 102/185 (55%), Gaps = 14/185 (7%) Query: 17 KAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEVGNMGLPPKLLAQGVKD 76 +AVVF+ Y RINDP L +D +++L ++ GP G+PG AEV NM P L+ +GV Sbjct: 424 RAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVST 483 Query: 77 MVRISDARMSGTAYGTVVLHVAPEAAAGGPLGIVQDGDWIEMDCAAGRLHLDISDEEMAQ 136 + + D R SGT+ +L+ +PE+A GG L ++DGD + +D G ++ +S+EE+A+ Sbjct: 484 LPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELAR 543 Query: 137 RQTARAEKLAQAKPAMQGGYQSLYIDRVLQADEGCDFDFLVGCRGAXGCDFDFLVGCAAI 196 R ++E + P+ Q +Q +Y V Q + G + + +G + Sbjct: 544 R---KSEGIPPVPPS-QTPWQEIYRSTVGQLETGACMELALKYQGV----------AQTL 589 Query: 197 PKHSH 201 P+H+H Sbjct: 590 PRHNH 594 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 78.6 bits (192), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 57/169 (33%), Positives = 91/169 (53%), Gaps = 10/169 (5%) Query: 1 VLKPSAASPELMKHRGKAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEV 60 ++K + ++K G A VFE E I V A V++++ GPKG PGM E Sbjct: 439 IVKTAGVDESILKFSGTARVFESQDAAVEGILGDK--VKAGDVVIVRYEGPKGGPGMQE- 495 Query: 61 GNMGLPPKLL-AQGV-KDMVRISDARMSGTAYGTVVLHVAPEAAAGGPLGIVQDGDWIEM 118 M P + ++G+ K +D R SG + G V+ H +PEAA GG +G+V++GD I++ Sbjct: 496 --MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDI 553 Query: 119 DCAAGRLHLDISDEEMAQRQTARAEKLAQAKPAMQGGYQSLYIDRVLQA 167 D ++L IS +++A+R+TA A+ A Q G + + + LQA Sbjct: 554 DIPNRTINLRISSDDLAKRRTAME---ARGDAAWQPGNRQRVVSQALQA 599 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 65.5 bits (158), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 57/172 (33%), Positives = 81/172 (47%), Gaps = 7/172 (4%) Query: 1 VLKPSAASPELMKHRGKAVVFEDFAHYKERINDPDLDVDASSVLVMKNCGPKGYPGMAEV 60 V+K SA PE AVVF + LD D +V+ + GP+ GM E+ Sbjct: 440 VIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQLDRDFVAVITFQ--GPRAN-GMPEL 496 Query: 61 GNMGLPPKLLAQGVKDMVRISDARMSGTAYGTV--VLHVAPEAAAGGPLGIVQDGDWIEM 118 + +L + + ++D RMSG A G V +HV PE GGPLG V+DGD I + Sbjct: 497 HALTPALGILQDKGRHVALVTDGRMSG-ASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRL 555 Query: 119 DCAAGRLHLDISDEEMAQRQTARAEKLAQAKPAMQGGYQSLYIDRVLQADEG 170 D AG L + E A R ++ L+ + M SL+ + V A+EG Sbjct: 556 DAEAGTLEAKVDATEWAARSQDSSD-LSGNQHGMGRELFSLFRNAVGTAEEG 606 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 684,327 Number of extensions: 28560 Number of successful extensions: 77 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 72 Number of HSP's successfully gapped: 5 Length of query: 201 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 120 Effective length of database: 1,236,335 Effective search space: 148360200 Effective search space used: 148360200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)