BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0073.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [H... 400 e-113 gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 244 5e-66 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 115 3e-27 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 115 4e-27 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 114 8e-27 >gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 400 bits (1028), Expect = e-113, Method: Compositional matrix adjust. Identities = 202/252 (80%), Positives = 224/252 (88%) Query: 6 QLASYPSLKGKRVFITGGGTGIGAAIVSALAEQGAVVAFVDIAKDASEALCKQIADAGFT 65 QLAS+PSLKGKRVFITGGGTGIGAAIV A A+QGA VAFVDIA +ASEALC ++A AG Sbjct: 9 QLASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCNEVAAAGHP 68 Query: 66 APSFRYCDLRDIASLQNTIRELAAQLGDFDVLVNNAANDERHKLEDVTQEYWDNRIAINQ 125 P FR+CDLRDI + Q TI EL AQLGDFDVLVNNAAND+RHKLE+VT EYW++RIAINQ Sbjct: 69 KPLFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYWNDRIAINQ 128 Query: 126 RPSFFAVQAIAPGMIKKKAGSIINFSSISWHTSSGGFPVYTTAKSSVLGLTRGLARDLGA 185 RPSFFAVQ++ GM ++ GSIINFSSISWH S GGFPVYTTAK+S LGLTRGLARDLG Sbjct: 129 RPSFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKASTLGLTRGLARDLGP 188 Query: 186 HNIRVNTVTPGWVMTERQIELWLDEKGKEDIARNQCLQGELLPWHLARMVLFLASDESVM 245 H IRVNTVTPGWVMTERQI+LWLDE+GK+ IARNQCLQG+LLPWHLARMVLFLA+D+S M Sbjct: 189 HKIRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLFLAADDSAM 248 Query: 246 CTAQEFIVDAGW 257 CTAQEFIVDAGW Sbjct: 249 CTAQEFIVDAGW 260 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 244 bits (623), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 120/256 (46%), Positives = 161/256 (62%), Gaps = 1/256 (0%) Query: 2 SATYQLASYPSLKGKRVFITGGGTGIGAAIVSALAEQGAVVAFVDIAKDASEALCKQIAD 61 + ++ A Y SL GKRV ITGGG+GIGAA+V A QGA V F+DIA + S+AL + D Sbjct: 6 TPAFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALVASLKD 65 Query: 62 AGFTAPSFRYCDLRDIASLQNTIRELAAQLGDFDVLVNNAANDERHKLEDVTQEYWDNRI 121 A AP F C+L ++ +L+ T E+ +G D+L+NNAAND+RHK DVT YWD R+ Sbjct: 66 API-APRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYWDERL 124 Query: 122 AINQRPSFFAVQAIAPGMIKKKAGSIINFSSISWHTSSGGFPVYTTAKSSVLGLTRGLAR 181 A+N R FF QA+ PGM ++K G I+NF SISWH +Y TAK+ + G+T G+AR Sbjct: 125 AVNLRHQFFCAQAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMAR 184 Query: 182 DLGAHNIRVNTVTPGWVMTERQIELWLDEKGKEDIARNQCLQGELLPWHLARMVLFLASD 241 D G +RVN + PG + T RQ LW + + I QCL + P +A + LFL+SD Sbjct: 185 DFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALFLSSD 244 Query: 242 ESVMCTAQEFIVDAGW 257 CT +E+ VDAGW Sbjct: 245 SGAKCTGREYYVDAGW 260 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 115 bits (288), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 91/260 (35%), Positives = 129/260 (49%), Gaps = 31/260 (11%) Query: 13 LKGKRVFITGGG--TGIGAAIVSALAEQGAVVAFVDIAKDASEALCKQIADA--GFTAPS 68 LK K ITGG G+G A +AEQGA V +D+A+ A +I G A Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVA-- 60 Query: 69 FRYCDLRDIASLQNTIRELAAQLGDFDVLVNNAANDERHKLEDVTQEYWDNRIAINQRPS 128 D+ D AS + + Q G D+LVNNA + K ++T +D + ++ R + Sbjct: 61 ----DVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGT 116 Query: 129 FFAVQAIAPGMIKKKAGSIINFSSISWHTSSG--GFPVYTTAKSSVLGLTRGLARDLGAH 186 + QA+ P M ++ GSII SS+S G G P Y+ AK+ VLGL R +AR+ G Sbjct: 117 LYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPE 176 Query: 187 NIRVNTVTPGWVMTERQIELWLDEKGKEDIARNQCLQGELLPWHLARM---------VLF 237 IRVN++TPG + T+ I+ L E+ K +IA E +P LAR+ +F Sbjct: 177 GIRVNSITPGLIGTD-IIKGKLTEEKKAEIA-------ETIP--LARLGRADDIGGACVF 226 Query: 238 LASDESVMCTAQEFIVDAGW 257 L SD S CT V+ G Sbjct: 227 LGSDLSQYCTGITLDVNGGM 246 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 115 bits (288), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 84/251 (33%), Positives = 127/251 (50%), Gaps = 13/251 (5%) Query: 13 LKGKRVFITGGG--TGIGAAIVSALAEQGAVVAFVDI--AKDASEALCKQIADAGFTAPS 68 LK K ITGG G+G + +A QGA + +D+ A+ A A G A Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVA-- 60 Query: 69 FRYCDLRDIASLQNTIRELAAQLGDFDVLVNNAANDERHKLEDVTQEYWDNRIAINQRPS 128 D+ D A+ + + Q G D+LVNNA + KL ++T + +D + ++ R + Sbjct: 61 ----DVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGT 116 Query: 129 FFAVQAIAPGMIKKKAGSIINFSSISWHTSSG--GFPVYTTAKSSVLGLTRGLARDLGAH 186 + QA+ P M +++GSI+ SS+S G G P Y+ AK+ VLGL R +AR+ G Sbjct: 117 LYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQ 176 Query: 187 NIRVNTVTPGWVMTERQIELWLDEKGKEDIARNQCLQGELLPWHLARMVLFLASDESVMC 246 IRVN +TPG + T+ ++ EK K +IA+ L P + + +FLASD S C Sbjct: 177 GIRVNCITPGLISTDINKDILSAEK-KAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYC 235 Query: 247 TAQEFIVDAGW 257 T V+ G Sbjct: 236 TGVTLDVNGGM 246 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 114 bits (285), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 92/258 (35%), Positives = 125/258 (48%), Gaps = 20/258 (7%) Query: 9 SYPSLKGKRVFITGGGTGIGAAIVSALAEQGAVVAFVDIAK-DASEALCKQIADAGFTAP 67 S +L K F+ GG GIGAAIV LA +GA VAF ++ D ++AL + G A Sbjct: 11 STSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRAL 70 Query: 68 SFRYCDLRDIASLQNTIRELAAQLGDFDVLVNNAANDERHKLEDVTQEYWDNRIAINQRP 127 + + D D A+LQ IR A G D+LVNNA L+ E D +A+N R Sbjct: 71 AIQ-ADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRS 129 Query: 128 SFFAVQAIAPGMIKKKAGSIINFSSISW-HTSSGGFPVYTTAKSSVLGLTRGLARDLGAH 186 F A Q A M + G +I+ S + G VY +KS+++GLT+GLARDLG Sbjct: 130 VFVATQEAARYM--GEGGRVISIGSTNADRIPFAGGAVYAMSKSALVGLTKGLARDLGPR 187 Query: 187 NIRVNTVTPGWVMTERQIELWLDEKGKEDIARNQCLQGEL-LPWH-----LARMVLFLAS 240 I VN V PG V T+ D A L G + LP + +A V +LA Sbjct: 188 GITVNNVQPGPVDTDM-------NPASGDFA--ASLTGLMALPRYGKAEEIASFVAYLAG 238 Query: 241 DESVMCTAQEFIVDAGWA 258 ES T ++D G++ Sbjct: 239 PESGYITGANLMIDGGFS 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 709,637 Number of extensions: 26798 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 44 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)