BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0018.p (248 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 56 3e-09 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 55.8 bits (133), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 58/177 (32%), Positives = 79/177 (44%), Gaps = 18/177 (10%) Query: 10 GECMIELRPTHLLQPRHDAQHMPRAMAQSFSGDVYNTAVYFRRRSRQIESQFISAAGNDS 69 GE M+E R DA PR Q + GD N + S ++SA G+D Sbjct: 9 GEAMVEF------NQRIDA---PRQFLQGYGGDTSN--FAIAAARQGAASAYLSAVGDDH 57 Query: 70 ISASLRDEVTRQGIGVSLMAAHPDALPGLYWIETNAAGERSFLYWRQQSAARHMLDEAHA 129 L R+ + V +A P G+Y++ +AAG F Y R SAA A Sbjct: 58 FGQDLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHH-FHYRRAGSAASAY----QA 112 Query: 130 DAL-MQQAAHCSLLYFSGITLAILDHARRRRLLDLAQAVRAHGGWVAFDSNYRPRLW 185 AL ++ A L+ SGI+LAI + A L +A A RA G + D+N R RLW Sbjct: 113 KALPLEAIAAAGALHLSGISLAISESACEAGLAAMAHA-RASGVTTSLDTNLRLRLW 168 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.131 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 703,527 Number of extensions: 24610 Number of successful extensions: 137 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 1 Length of query: 248 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 165 Effective length of database: 1,226,865 Effective search space: 202432725 Effective search space used: 202432725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)