BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0748.p (1253 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313633|ref|YP_003777725.1| methionine synthase I, cobalami... 2352 0.0 >gi|300313633|ref|YP_003777725.1| methionine synthase I, cobalamin-binding domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1247 Score = 2352 bits (6095), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1120/1247 (89%), Positives = 1186/1247 (95%) Query: 6 MKPNDPCQTELLLRDLMSQRILILDGAMGTMIQQYKLTEEDYRGERFKDFSVPGKELFVK 65 MKPN+ CQTELLLRDLMSQRILILDGAMGTMIQ+YKLTEEDYRG+RF DFSVPGK+LFVK Sbjct: 1 MKPNELCQTELLLRDLMSQRILILDGAMGTMIQRYKLTEEDYRGQRFADFSVPGKDLFVK 60 Query: 66 GNNELLSLTQPHIIQEIHEQYLAAGADLVETNTFGATGVAQDDYHMAHLVYEMNVQSAKL 125 GNNELLSLTQPHIIQEIHEQYLAAGADL+ETNTFGAT VAQDDYHMAHLVYEMNV+SA+L Sbjct: 61 GNNELLSLTQPHIIQEIHEQYLAAGADLIETNTFGATSVAQDDYHMAHLVYEMNVESARL 120 Query: 126 ARAACDKYSTPDKPRFVAGALGPTPKTASISPDVNDPAARNVTFDQLVASYLEQTRALVE 185 ARAACDKY+TPDKPRFVAGALGPTPKTASISPDVNDPAARNVTFDQLVA+YLEQTRALVE Sbjct: 121 ARAACDKYATPDKPRFVAGALGPTPKTASISPDVNDPAARNVTFDQLVAAYLEQTRALVE 180 Query: 186 GGADVLLVETIFDTLNCKAALFAIDVFFEESGKRLPIMISGTVTDASGRILSGQTVPAFW 245 GGADVLLVETIFDTLNCKAALFAID FFEESG+RLPIMISGTVTDASGRILSGQTV AFW Sbjct: 181 GGADVLLVETIFDTLNCKAALFAIDTFFEESGQRLPIMISGTVTDASGRILSGQTVTAFW 240 Query: 246 HSIRHARPLTVGLNCALGAALMRPYAEELSKIADTFVCIYPNAGLPNPMSDTGFDETPDV 305 +SIRHARPLTVGLNCALGAALMRPYAEELSKIADTFVCIYPNAGLPNPMSDTGFDETPDV Sbjct: 241 NSIRHARPLTVGLNCALGAALMRPYAEELSKIADTFVCIYPNAGLPNPMSDTGFDETPDV 300 Query: 306 TSSLLKDFAESGFVNVAGGCCGTTPAHIKAIADTVATIAPRQVPEGKHEMLLSGLEPFII 365 TS+LLK+FAESGFVNVAGGCCGTTP HIKAIA+TVA IAPR+VPE HEM LSGLEPF I Sbjct: 301 TSALLKEFAESGFVNVAGGCCGTTPPHIKAIAETVAKIAPRKVPEPTHEMRLSGLEPFTI 360 Query: 366 NDQSLYVNVGERTNVTGSKAFARLILNEQYDEALAVARQQVENGAQIIDINMDEAMLDSL 425 ND SLYVNVGERTNVTGSKAFARLILNEQYDEALAVARQQVENGAQIIDINMDEAMLDS+ Sbjct: 361 NDDSLYVNVGERTNVTGSKAFARLILNEQYDEALAVARQQVENGAQIIDINMDEAMLDSV 420 Query: 426 AAMTRFLNLIASEPDIARVPVMIDSSKWSVIEAGLKCVQGKSIVNSISMKEGEEKFLHEA 485 AAMTRFLNLIASEPDIARVP+MIDSSKW VIEAGLKCVQGKSIVNSISMKEGEEKFL EA Sbjct: 421 AAMTRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKSIVNSISMKEGEEKFLREA 480 Query: 486 KLCRRYGAAVIVMAFDEVGQADTYERKIEICERAYRLLVDKVGFPPEDIIFDPNIFAVAT 545 KLCRRYGAAVIVMAFDEVGQADT+ RKIEICERAYRLLVDK+ FPPEDIIFDPNIFAVAT Sbjct: 481 KLCRRYGAAVIVMAFDEVGQADTFARKIEICERAYRLLVDKLDFPPEDIIFDPNIFAVAT 540 Query: 546 GIEEHNNYAVDFIEATRWIHQNLPYAKISGGVSNVSFSFRGNDPAREAIHTVFLYHAVKA 605 GIEEHNNYAVDFIEATRWIHQNLPYAKISGGVSNVSFSFRGNDPAREAIHTVFLYHA+KA Sbjct: 541 GIEEHNNYAVDFIEATRWIHQNLPYAKISGGVSNVSFSFRGNDPAREAIHTVFLYHAIKA 600 Query: 606 GMTMGIVNAGMIGVYDDLPAELREAVEDVVLNRRPDATERMIDIAATLKAGDKKEEATLE 665 GMTMGIVNAGMIGVYDDLPAELRE VEDVVLNRR DATERMI+ AATLKAGDKKEEATLE Sbjct: 601 GMTMGIVNAGMIGVYDDLPAELRERVEDVVLNRREDATERMIEYAATLKAGDKKEEATLE 660 Query: 666 WRSEPVEKRLAHALVLGITQWIVEDTEEARQNLLKSGGRPIHVIEGPLMDGMNVVGDLFG 725 WR+ PV KRL+HALV GITQWIVEDTEE RQ + GGRPIHVIEGPLMDGMNVVGDLFG Sbjct: 661 WRNLPVAKRLSHALVHGITQWIVEDTEEVRQQIAADGGRPIHVIEGPLMDGMNVVGDLFG 720 Query: 726 QGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLIEQETGIVAKPKGKIVIATVKGDVHDI 785 QGKMFLPQVVKSARVMKQAVAHLIPFIEEEKR +E TG VAKPKGKIVIATVKGDVHDI Sbjct: 721 QGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRQLEIATGEVAKPKGKIVIATVKGDVHDI 780 Query: 786 GKNIVSVVLQCNNFEVVNMGVMVPCSQILAKAKEENADIIGLSGLITPSLEEMAYVAKEM 845 GKNIV+VVLQCNNFEVVNMGVMVPC++ILAKAKEE ADIIGLSGLITPSLEEMAYVAKEM Sbjct: 781 GKNIVTVVLQCNNFEVVNMGVMVPCAEILAKAKEEKADIIGLSGLITPSLEEMAYVAKEM 840 Query: 846 QRDSYFSGVKMPLLIGGATTSRAHTAVKIAPNYSGPVVYVPDASRAVSVAQSLLTPEQKD 905 QRD YF+G+KMPL+IGGATTSRAHTAVKIAPNY GPVVYVPDASRAVSVAQSLL EQK Sbjct: 841 QRDPYFAGLKMPLMIGGATTSRAHTAVKIAPNYEGPVVYVPDASRAVSVAQSLLADEQKT 900 Query: 906 QYIADIATDYERIREQHANKKAVPLVPIAAARDNKMQLEFAPVKPKFIGRRVFKNVDLAT 965 QY+A++ DYERIREQHA+KKA P++ +AAAR NK +L+FAPVKPKFIGRR+FKNVDL Sbjct: 901 QYVAELNADYERIREQHASKKAAPMLSLAAARANKTKLDFAPVKPKFIGRRLFKNVDLGL 960 Query: 966 LAKYIDWGPFFQTWDLAGPYPAILKDEVVGEAASKVFEEAQALLKKVIDGRWLTANGIVS 1025 LA YIDWGPFFQTWDLAGPYPAIL DEVVGEAA+KVF+EAQA+LKK+IDGRWLTANG++S Sbjct: 961 LANYIDWGPFFQTWDLAGPYPAILTDEVVGEAATKVFQEAQAMLKKIIDGRWLTANGVIS 1020 Query: 1026 LLPANTVNDDDIEIYTDDTRTQVALTYYGVRQQTEKPVIDGVARPNQCLADFIAPKASGI 1085 LLPANTVNDDDIEIYTDD+R+QVA TYYG+RQQTEKPV+DGVARPNQCL+DFIAPK SG+ Sbjct: 1021 LLPANTVNDDDIEIYTDDSRSQVAFTYYGLRQQTEKPVVDGVARPNQCLSDFIAPKESGV 1080 Query: 1086 ADYIGMFAVTSGLGIEKYEKRFEEAHDDYSSIMLKALADRLAEAFAEYLHERVRTDLWGY 1145 DYIGMFAVT+GLGIEKYEKRFE+AHDDYSSIMLKALADRLAEAFAEYLHERVR DLWGY Sbjct: 1081 QDYIGMFAVTAGLGIEKYEKRFEDAHDDYSSIMLKALADRLAEAFAEYLHERVRKDLWGY 1140 Query: 1146 ASQESLSAEELIKEKYVGIRPAAGYPACPEHTVKAEAFRVMQCDEIGMTVTESYAMFPGA 1205 A+ E+LS+ +LIKEKY+GIRPA GYPACPEHTVKA+ FR MQCDEIGM +TESYAMFPGA Sbjct: 1141 AADENLSSTDLIKEKYLGIRPAPGYPACPEHTVKADVFRTMQCDEIGMQLTESYAMFPGA 1200 Query: 1206 SVSGFYFAHPDSKYFVVGKIGDDQVADMAERRGVSKDDVERWLAPNL 1252 SVSGFYFAHP SKYFVVGKIG+DQV DMAERR V K+++ERWLAPNL Sbjct: 1201 SVSGFYFAHPQSKYFVVGKIGEDQVVDMAERRHVPKEELERWLAPNL 1247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,928,691 Number of extensions: 168900 Number of successful extensions: 518 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 518 Number of HSP's successfully gapped: 1 Length of query: 1253 Length of database: 1,619,870 Length adjustment: 96 Effective length of query: 1157 Effective length of database: 1,165,310 Effective search space: 1348263670 Effective search space used: 1348263670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 136 (57.0 bits)