BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0732.p (544 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313617|ref|YP_003777709.1| translation elongation factor [... 910 0.0 gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspir... 779 0.0 gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 ... 89 1e-18 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 62 2e-10 >gi|300313617|ref|YP_003777709.1| translation elongation factor [Herbaspirillum seropedicae SmR1] Length = 701 Score = 910 bits (2351), Expect = 0.0, Method: Compositional matrix adjust. Identities = 438/502 (87%), Positives = 472/502 (94%), Gaps = 4/502 (0%) Query: 1 MVYDSVGGVQPQSETVWRQANKYGVPRMAFVNKMDRVGADFFRVQKQIGERLKGNAVPIQ 60 MVYDSVGGVQPQSETVWRQANKY VPR+AFVNKMDR+GADFFRVQKQI ERLKG AVPIQ Sbjct: 111 MVYDSVGGVQPQSETVWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERLKGKAVPIQ 170 Query: 61 IPIGAEDHFQGVIDLVKMKAIIWDEASQGVKFEYQPIPVELEETAREWHDKMIEQAAEAN 120 IP+GAEDHF GVIDLVKMKAIIWDEASQGV F+Y+ IP+ELE+TAR W D M+EQAAEAN Sbjct: 171 IPVGAEDHFSGVIDLVKMKAIIWDEASQGVLFKYEDIPLELEDTARHWRDLMVEQAAEAN 230 Query: 121 EQLLEKYLSGTPLTEEDVKQGLRLRTIAGEI----XGSAFKNKGVQAMLDAVIDYLPSPI 176 E+LLEKYLSG PLTE+D+K+GLRLRT+A EI GSAFKNKGVQAMLDAVIDYLPSP+ Sbjct: 231 EELLEKYLSGNPLTEDDIKRGLRLRTVANEIVPMLAGSAFKNKGVQAMLDAVIDYLPSPV 290 Query: 177 DVPAIKGHDEEDNEIERHPADDEKFSALAFKIMTDPFVGQLIFFRVYSGMINSGDTVYNP 236 DVPAI GH E+D+EIERHP+D+E FSALAFKIMTDPFVGQL FFRVYSG++NSGDTVYNP Sbjct: 291 DVPAIAGHGEDDSEIERHPSDEEPFSALAFKIMTDPFVGQLTFFRVYSGIVNSGDTVYNP 350 Query: 237 IKGKKERLGRILQMHANERKEIKEVYAGDIAAAVGLKDVTTGDTLSDPDDVIILERMVFP 296 +KGKKERLGRILQMHANERKEIKEV+AGDIAAAVGLKDVTTGDTLSDP+ IILERM+FP Sbjct: 351 VKGKKERLGRILQMHANERKEIKEVFAGDIAAAVGLKDVTTGDTLSDPEHPIILERMIFP 410 Query: 297 EPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVRTDEESGQTIISGMGELHLEILVDR 356 EPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRV TDEESGQTI+SGMGELHLEILVDR Sbjct: 411 EPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVHTDEESGQTIMSGMGELHLEILVDR 470 Query: 357 MKREFNVEATVGKPQVAYREAIRKTIEDVEGKFVKQSGGRGQYGHVVLKLEPLPPGTGYE 416 MKREFNVEATVGKPQVAYREAIRK +EDVEGKFVKQSGGRGQYGHVV+KLEP P G GYE Sbjct: 471 MKREFNVEATVGKPQVAYREAIRKAVEDVEGKFVKQSGGRGQYGHVVIKLEPQPAGKGYE 530 Query: 417 FIDAIKGGVVPREFIPAVDKGIQETLGSGVLAGYPVVDIRATLTFGSYHDVDSNENAFRM 476 F+DAIKGGVVPREFIPAVDKGIQE+L +GVLAGYPVVD++ATLTFGSYHDVDSNENAFRM Sbjct: 531 FVDAIKGGVVPREFIPAVDKGIQESLKAGVLAGYPVVDVKATLTFGSYHDVDSNENAFRM 590 Query: 477 AGSMAFKEGMRRASPVLLEPMM 498 AGSMAFKE M+RA P+LLEPMM Sbjct: 591 AGSMAFKEAMKRAGPMLLEPMM 612 >gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspirillum seropedicae SmR1] Length = 701 Score = 779 bits (2011), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/502 (73%), Positives = 432/502 (86%), Gaps = 4/502 (0%) Query: 1 MVYDSVGGVQPQSETVWRQANKYGVPRMAFVNKMDRVGADFFRVQKQIGERLKGNAVPIQ 60 MVY +VGGVQPQSETVWRQANKY VPR+AFVNKMDR GA+FF+V +Q+ RLK N +P+Q Sbjct: 111 MVYCAVGGVQPQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYEQMRARLKANPIPMQ 170 Query: 61 IPIGAEDHFQGVIDLVKMKAIIWDEASQGVKFEYQPIPVELEETAREWHDKMIEQAAEAN 120 +PIGAE++F+GVIDLVKM+AI WD+ASQG+KF+Y+ IP L+ A++W + ++E AAEA+ Sbjct: 171 VPIGAEENFEGVIDLVKMRAIYWDDASQGMKFDYRDIPEHLKADAQKWRENLVETAAEAS 230 Query: 121 EQLLEKYLSGTPLTEEDVKQGLRLRTIAGEI----XGSAFKNKGVQAMLDAVIDYLPSPI 176 E L+ KYL LTE ++K +R RTI+GEI G+AFKNKGVQAMLD V++YLPSP+ Sbjct: 231 EDLMNKYLEEGDLTEAEIKGAIRQRTISGEIVPMMCGTAFKNKGVQAMLDGVVEYLPSPV 290 Query: 177 DVPAIKGHDEEDNEIERHPADDEKFSALAFKIMTDPFVGQLIFFRVYSGMINSGDTVYNP 236 D+P + G +E+D + R DDEKFSALAFKI TDPFVGQL F R YSG +NSGDTV N Sbjct: 291 DIPPVPGLNEDDEPVVREAKDDEKFSALAFKIATDPFVGQLCFIRCYSGTLNSGDTVLNS 350 Query: 237 IKGKKERLGRILQMHANERKEIKEVYAGDIAAAVGLKDVTTGDTLSDPDDVIILERMVFP 296 +K KKER+GRI+QMHAN+R+EIKE+ AGDIAA VGLKD TTGDTL D +++LERMVFP Sbjct: 351 VKSKKERIGRIVQMHANQREEIKEMMAGDIAAVVGLKDTTTGDTLCDDKAMVVLERMVFP 410 Query: 297 EPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVRTDEESGQTIISGMGELHLEILVDR 356 EPVISQAVEPKTKADQEKMG+ALNRLA EDPSFRVRTDEESGQTII+GMGELHL+I+VDR Sbjct: 411 EPVISQAVEPKTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDR 470 Query: 357 MKREFNVEATVGKPQVAYREAIRKTIEDVEGKFVKQSGGRGQYGHVVLKLEPLPPGTGYE 416 MKREFNVEATVGKPQVAYRE IRKT E+ EGKFVKQSGGRGQYGHVVLK+EP G G+E Sbjct: 471 MKREFNVEATVGKPQVAYRETIRKTCEESEGKFVKQSGGRGQYGHVVLKIEPQEAGKGFE 530 Query: 417 FIDAIKGGVVPREFIPAVDKGIQETLGSGVLAGYPVVDIRATLTFGSYHDVDSNENAFRM 476 F+DAIKGG VPREFIPAV+KG++ETL +GVLAGYPVVD++ TL FGSYHDVDSNENAFRM Sbjct: 531 FVDAIKGGTVPREFIPAVEKGVRETLNTGVLAGYPVVDVKVTLFFGSYHDVDSNENAFRM 590 Query: 477 AGSMAFKEGMRRASPVLLEPMM 498 AGSMAFKEG RRASPV+LEPMM Sbjct: 591 AGSMAFKEGCRRASPVILEPMM 612 >gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 [Herbaspirillum seropedicae SmR1] Length = 530 Score = 88.6 bits (218), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 93/413 (22%), Positives = 173/413 (41%), Gaps = 51/413 (12%) Query: 1 MVYDSVGGVQPQSETVWRQANKYGVPRMAFVNKMDRVGADFFRVQKQIGERLKGNAVPIQ 60 MV D+ GV+ Q+ + P + F+NK+DR D + ++ LK P+ Sbjct: 107 MVIDAAKGVEEQTIKLLNVCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCAPVT 166 Query: 61 IPIGAEDHFQGVIDLVKMKAIIWDEASQGVKFEYQPI------------PVELEETAREW 108 PIG +F+GV L++ + +++ + + I P+E+E+ E Sbjct: 167 WPIGMGKNFRGVYHLLRDEIMLFKAGEERANGNVEIIKGIDNPKLAEMFPLEIEQLKME- 225 Query: 109 HDKMIEQAAEANEQL-LEKYLSG--TPLTEEDVKQGLRLRTIAGEIXGSAFKNKGVQAML 165 +E A+ L+ +L+G TP+ GSA N GV+ +L Sbjct: 226 ----VELVHGASHPFNLDDFLAGVQTPV-----------------FFGSAINNFGVREIL 264 Query: 166 DAVIDYLPSPIDVPAIKGHDEEDNEIERHPADDEKFSALAFKIMT--DP-FVGQLIFFRV 222 DA++D+ P P G D E++ +E FS FKI DP ++ F RV Sbjct: 265 DALLDWAPGP------GGRDATVREVK---PTEEPFSGFVFKIQANMDPAHRDRIAFLRV 315 Query: 223 YSGMINSGDTVYNPIKGKKERLGRILQMHANERKEIKEVYAGDIAAAVGLKDVTTGDTLS 282 SG G + + + ++ ++ A+ R++++E YAGDI ++ GD+ S Sbjct: 316 CSGRFERGMKLKHLRLNRDIKVSSVVTFMASSREQVEEAYAGDIIGLPNHGNMQIGDSFS 375 Query: 283 DPDDVIILERMVFPEPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVRTDEESGQTII 342 + +++ + + P + + +++ L +L +E + +V I+ Sbjct: 376 E-GEMLQFTGIPYFAPDFFRVARIRNPLKIKQLHKGLQQLGEEG-AVQVFKPVTGSDLIL 433 Query: 343 SGMGELHLEILVDRMKREFNVEATVGKPQVAYREAIRKTIEDVEGKFVKQSGG 395 +G L E++ R+ E+ V+A ++ I + F K S G Sbjct: 434 GAVGVLQFEVVASRLLNEYGVDAVFEGTSISSARWISCDDKKKLADFEKSSAG 486 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 61.6 bits (148), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 59/229 (25%), Positives = 99/229 (43%), Gaps = 33/229 (14%) Query: 161 VQAMLDAVIDYLPSPIDVPAIKGHDEEDNEIERHPADDEKFSALAFKIMTDPFVGQLIFF 220 ++ + DA++ Y+P+ D P D + +VG++ Sbjct: 200 MEPLFDAILKYVPARKDDP------------------DAPLQLQITSLEYSSYVGKIGVG 241 Query: 221 RVYSGMINSG-DTVYN---PIKGKKERLGRILQMHANERKEIKEVYAGDIAAAVGLKDVT 276 R+ +G + G D V+ K K R+ ++L ER + E AGDI G++++ Sbjct: 242 RILAGRVKGGQDVVWMNGPDDKPTKARINQVLTFKGLERVLVDEALAGDIVLINGIEEIG 301 Query: 277 TGDTLSDPDDVIILERMVFPEPVISQAVEPKTKADQEKMGIAL------NRLAQE---DP 327 G T+ PD L + EP ++ T + G + +RL +E + Sbjct: 302 IGSTICSPDAPNGLPMLKVDEPTLTMNFMVNTSPLAGREGKFVTTRQIRDRLEKELKSNM 361 Query: 328 SFRVRTDEESGQTI-ISGMGELHLEILVDRMKREFNVEATVGKPQVAYR 375 + RV E T +SG GELHL IL++ M+RE E V +P+V +R Sbjct: 362 ALRVVQAENDDSTYEVSGRGELHLTILIENMRRE-GFELAVSRPRVVFR 409 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,666,120 Number of extensions: 72797 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 4 Length of query: 544 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 454 Effective length of database: 1,193,720 Effective search space: 541948880 Effective search space used: 541948880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)