BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0704.p (221 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313592|ref|YP_003777684.1| alpha/beta superfamily hydrolas... 333 5e-93 >gi|300313592|ref|YP_003777684.1| alpha/beta superfamily hydrolase [Herbaspirillum seropedicae SmR1] Length = 220 Score = 333 bits (855), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 174/223 (78%), Positives = 188/223 (84%), Gaps = 12/223 (5%) Query: 1 MNAQTQAFLIPGAAGQLECALDLPDSEQFAAPRARARGXRAPRGLALVAHPHPLYGGTMD 60 MNA TQ F+I GA G LECALDLPD E F+ P GLALVAHPHPL+GGTMD Sbjct: 1 MNAHTQNFMITGAVGALECALDLPDPETFSTPV----------GLALVAHPHPLFGGTMD 50 Query: 61 NKVAQTLARSFLALGYVTVRMNFRGVGKSAGVHDHGHGETDDMALLLAHMRTQYPDLPVT 120 NKVAQTLAR+FLALGYV VRMNFRGVGKS GVHDHG GETDDMALLL HMR+QYPDLP+ Sbjct: 51 NKVAQTLARTFLALGYVAVRMNFRGVGKSEGVHDHGAGETDDMALLLQHMRSQYPDLPLA 110 Query: 121 LGGFSFGTFVQAQLQQRLIAE--GTPAERLALVGTAAGKWPMPPVPADTILIHGELDDTI 178 L GFSFGTFVQAQLQQRL+ + + AERL LVGTAAGKWPMPP PADTILIHGE DDTI Sbjct: 111 LAGFSFGTFVQAQLQQRLLQQDPASAAERLVLVGTAAGKWPMPPAPADTILIHGEQDDTI 170 Query: 179 PLTAVLDWARPQELPVIVIPGADHFFHRKLQHIKTLIVQMWHR 221 PL+AVLDWARPQELPV+VIPG+DHFFHRKLQHIK ++VQ WHR Sbjct: 171 PLSAVLDWARPQELPVVVIPGSDHFFHRKLQHIKNVVVQSWHR 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 738,871 Number of extensions: 31271 Number of successful extensions: 102 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 100 Number of HSP's successfully gapped: 1 Length of query: 221 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 139 Effective length of database: 1,231,600 Effective search space: 171192400 Effective search space used: 171192400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)