BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0677.p (232 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herb... 390 e-110 gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herb... 167 8e-43 gi|300313883|ref|YP_003777975.1| glutathione S-transferase [Herb... 63 2e-11 >gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 233 Score = 390 bits (1003), Expect = e-110, Method: Compositional matrix adjust. Identities = 182/232 (78%), Positives = 201/232 (86%) Query: 1 MQNTELDPTLSATLEKAGKNAITLVIGNKNYSSWSMRPWVVLTAFDIPFREIRICLDQPD 60 MQ+TELDP LS TL+KAG++ TLVI NKNYSSWSMRPWV + AFDIPF+E+R+ LDQ D Sbjct: 1 MQDTELDPVLSETLQKAGRHLPTLVIANKNYSSWSMRPWVAMVAFDIPFKEVRLLLDQAD 60 Query: 61 TAGKIAEYSAAGRVPVLLDGQTHIWDSLAICEYLAEQFPDRKLWPQETAARAVARSITAE 120 TA +IA Y+ +GRVPVLLDG IWDSL+ICEYLAEQFPDR LWP T ARA+ARSI AE Sbjct: 61 TAAEIARYTGSGRVPVLLDGDIAIWDSLSICEYLAEQFPDRHLWPARTDARAIARSICAE 120 Query: 121 MHSGFTGLRSAMSMDIRGSYPGKGRTAEAQGDIGRICEIWEDCLSQFGHHEFLFGDFSIA 180 MHSGFTGLRSAMSMDIRG YPG+GRTAEAQ DI R+CEIWE+CLSQFGHH+FLFGDFSIA Sbjct: 121 MHSGFTGLRSAMSMDIRGRYPGQGRTAEAQADIARVCEIWEECLSQFGHHDFLFGDFSIA 180 Query: 181 DAFYAPVVMRFRSYHASLAPALQAYVDRVAAHPAVAKWIAEALVEKEVLPDH 232 DAFYAPVVMRF SY LAPALQAY DRV HPAVA+WIAEAL EKE+LPDH Sbjct: 181 DAFYAPVVMRFISYQVPLAPALQAYADRVREHPAVARWIAEALAEKEILPDH 232 >gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 218 Score = 167 bits (422), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 90/206 (43%), Positives = 124/206 (60%), Gaps = 2/206 (0%) Query: 23 TLVIGNKNYSSWSMRPWVVLTAFDIPFREIRICLDQPDTAGKIAEYSAAGRVPVLLDGQT 82 TL I +KNYSSWS+R W++ + F E + D P +I S++ VP L G Sbjct: 5 TLRITSKNYSSWSLRGWLMARFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLHHGDV 64 Query: 83 HIWDSLAICEYLAEQFPDRKLWPQETAARAVARSITAEMHSGFTGLRSAMSMDIRGSYPG 142 +WD+LAI EYL E P KL P + AARA RS+ EMHSGF+ LR+A+ M+++G +P Sbjct: 65 LVWDTLAIAEYLNEIRPAAKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKGHFPN 124 Query: 143 KGRTAEAQGDIGRICEIWEDCLSQFGHHEFLFGD-FSIADAFYAPVVMRFRSYHASLAPA 201 + A+ DI RI IW++CL+++G FLFG+ ++ADA YAPVV RF +Y L P Sbjct: 125 FKVWSRAEADIVRITTIWKECLAKYG-GPFLFGEQRTVADAMYAPVVTRFMTYDVKLDPI 183 Query: 202 LQAYVDRVAAHPAVAKWIAEALVEKE 227 AY R+ A P + +WI A E E Sbjct: 184 CTAYAKRIMAMPEMQEWITAAKKEPE 209 >gi|300313883|ref|YP_003777975.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 225 Score = 62.8 bits (151), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 57/207 (27%), Positives = 91/207 (43%), Gaps = 10/207 (4%) Query: 20 NAITLVIGNKNYSSWSMRPWVVLTAFDIPFREIRICLD-----QPDTAGKIAEYSAAGRV 74 + + L + + S ++M +V L +PF + LD +PD A + S RV Sbjct: 19 DVLQLYVDAQFASPYAMSVFVALREKQLPFTLHTLDLDRGQQHEPDYAQR----SLTSRV 74 Query: 75 PVLLDGQTHIWDSLAICEYLAEQFPDRKLWPQETAARAVARSITAEMHSGFTGLRSAMSM 134 P+L G + +S AI EYL E F +L+PQ+ ARA AR + A + S LR + Sbjct: 75 PMLRHGALTLSESSAITEYLDEVFAGPRLYPQDAVARARARQVQAWLRSDLMALREERNT 134 Query: 135 DIRGSYPGKGRTAEAQGDIGRICEIWEDCLSQFGHHEFLFGDFSIADAFYAPVVMRFRSY 194 + K +E + + L G + LFG + IAD A ++ R + Sbjct: 135 QVVFYGAKKAALSEEASAAAQRLFHFAGQLLPVG-ADSLFGQWCIADVDLALMLNRLALH 193 Query: 195 HASLAPALQAYVDRVAAHPAVAKWIAE 221 + P L+ Y R P+V W+ + Sbjct: 194 GDRVPPHLEDYARRQWQRPSVQAWLTQ 220 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 770,016 Number of extensions: 30862 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 103 Number of HSP's successfully gapped: 3 Length of query: 232 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 150 Effective length of database: 1,231,600 Effective search space: 184740000 Effective search space used: 184740000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)