BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0632.p (418 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314019|ref|YP_003778111.1| amino acid deacylase [Herbaspir... 377 e-105 gi|300312087|ref|YP_003776179.1| peptidase [Herbaspirillum serop... 71 1e-13 gi|300312510|ref|YP_003776602.1| acetylornithine deacetylase [He... 65 9e-12 gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 58 2e-09 >gi|300314019|ref|YP_003778111.1| amino acid deacylase [Herbaspirillum seropedicae SmR1] Length = 414 Score = 377 bits (967), Expect = e-105, Method: Compositional matrix adjust. Identities = 210/414 (50%), Positives = 273/414 (65%), Gaps = 8/414 (1%) Query: 4 SLQQSVVTFLDAEHAAQTRFLQELVRVPSDNPPGDCAAHAARAQVLLEQLGFDVEVHVVP 63 L Q + ++D + LQ+L+R+P+D PPG+ A HA L+ G+ E HVVP Sbjct: 5 QLSQRLCAWIDQHFDEEVGVLQQLIRIPTDTPPGNNAPHADAVAELVAGWGWAAEKHVVP 64 Query: 64 DELVKANGMISAANLIVRKRFGSGGPTIALNAHGDVVPPGLGWSKDPYGGEIVQDAEHGP 123 + V GM S NLIVR+ +G GGPT+ALNAHGDVVPPG GW+ DPYGGEI Sbjct: 65 AQQVADYGMQSITNLIVRRPYGDGGPTLALNAHGDVVPPGEGWTHDPYGGEIADGR---- 120 Query: 124 VMYGRGVAVSKSDFATYTWALLALIAAEKNGAALNGAIELQFTHDEEAGGDIGPKWILEQ 183 +YGR AVSKSDFA+Y +A AL E GA+L G +EL FT+DEE GG +GP W+LEQ Sbjct: 121 -IYGRAAAVSKSDFASYLFATRAL---EAVGASLRGGVELHFTYDEEFGGLLGPGWLLEQ 176 Query: 184 GLSKPDYAVSAGFAYGITSAHNGCLHLEVTVKGKQAHAAMPHTGIDAIEAATGILQALYA 243 L++PDY ++AGF+Y + +AHN CL E+TV G+ H +MP TG DA++AAT IL A+Y Sbjct: 177 KLTRPDYVLAAGFSYNVVTAHNACLQFEITVHGRATHGSMPETGHDALQAATAILNAIYG 236 Query: 244 YRAELAEKKSAVAGIDHATLNVGLIKGGINTNVVPDLASFRLDRRMIPEEAGFDAEGDLR 303 L E +SA+ GI H T+ VG I GG NTNVVP ++DRRMIPEE E +R Sbjct: 237 ALPGLKEIRSAIPGITHPTMIVGRIDGGTNTNVVPGKVVLKMDRRMIPEEDPSQVEAGVR 296 Query: 304 RVVDAAAARYPGIEAGVQRILLAEPLAELPGVEKLIGAFSRNAEAILGEPVKAHGVPLYT 363 ++++ A A PGI ++R+LLA L LPG EKL+ + RNA ++GE + +G LYT Sbjct: 297 KLIEDAVAGLPGIRVEIRRLLLARALRPLPGHEKLVESLLRNAREVMGETLTTNGSALYT 356 Query: 364 DARHYTRQGVPTILYGAGPRTLMEARGHNSDENLRLNDLLKATKVVTLALAELL 417 DAR Y QG+P +L+GAGPRTLME+ +DENL L+DL KATKVV L L + L Sbjct: 357 DARLYGEQGIPVVLFGAGPRTLMESNAKQADENLALDDLRKATKVVALMLLDFL 410 >gi|300312087|ref|YP_003776179.1| peptidase [Herbaspirillum seropedicae SmR1] Length = 395 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 80/316 (25%), Positives = 127/316 (40%), Gaps = 43/316 (13%) Query: 85 GSGGPTIALNAHGDVVP-PGLGWSKDPYGGEIVQDAEHGPVMYGRGVAVSKSDFATYTWA 143 GS I L+ H DVVP G W DP+ + E +Y RG D + Sbjct: 70 GSTEGGIVLSGHTDVVPVDGQKWDTDPF-----KLTEKDGALYARG----SCDMKGFIAT 120 Query: 144 LLALIAAEKNGAALNGAIELQFTHDEEAGGDIGP---KWILEQGLSKPDYAVSAGFAYGI 200 LAL+ E I L F+ DEE G P + I+++G+ V + + Sbjct: 121 ALALVP-EYLAMPRVKPIHLAFSFDEEIGCIGAPVMLEEIVKRGIKVDGCVVGEPTSMNV 179 Query: 201 TSAHNGCLHLEVTVKGKQAHAAMPHTGIDAIEAATGILQAL--YAYRAELAEKKSAVAGI 258 AH G V GK AH+++ G +AIE A ++ A+ +A + + Sbjct: 180 VVAHKGINVFACKVHGKSAHSSLTPQGCNAIEHAARLICAIRDFADGYKANGPYDQFFDV 239 Query: 259 DHATLNVGLIKGGINTNVVPDLASFRLDRRMIPEEAGFDAEGDLRRVVDAAAARYPGIEA 318 +T+ I+GGI N +P+L F + R +P + D + I+ Sbjct: 240 PFSTMTTNQIRGGIAVNTIPELCEFTYEFRNLPGMSVADIQAQ--------------IDK 285 Query: 319 GVQRILLAEPLAELPGVEKLIGAFSRN------AEAILGEPVKA-------HGVPLYTDA 365 + +LL + E P I F+ + +A + E V+A V T+A Sbjct: 286 YIAEVLLPKMRTEFPDARVEIDNFAGSPALEAVEQAAITELVRALTGDRQTRKVAYGTEA 345 Query: 366 RHYTRQGVPTILYGAG 381 + + G+PTI+ G G Sbjct: 346 GLFQQIGIPTIVCGPG 361 >gi|300312510|ref|YP_003776602.1| acetylornithine deacetylase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 65.5 bits (158), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 90/353 (25%), Positives = 141/353 (39%), Gaps = 70/353 (19%) Query: 83 RFGSGGPTIALNAHGDVVP-PGLGWSKDPYGGEIVQDAEHGPVMYGRGVAVSKSDFATYT 141 RFG+ + H DVVP G W DP+ + ++GRG A F Sbjct: 76 RFGT-----VFSGHTDVVPVTGQKWDTDPFVAHVADGK-----LFGRG-ACDMKGFIAIC 124 Query: 142 WALLALIAAEKNGAALNGAIELQFTHDEEAG-------------GDIGPKWIL--EQGLS 186 A L I + A L+ + F++DEE G DI P ++ E + Sbjct: 125 MARLPAI----DLARLHTPLHFSFSYDEEVGCLGVRELLADLQQNDIRPTGVIIGEPTMM 180 Query: 187 KPDYAVSAGFAYGITSAHNGCLHLEVTVKGKQAHAAMPHTGIDAIEAATGILQALYAYRA 246 +P AH G +V G AH++ PH GI++I+ A + L Sbjct: 181 QP------------VIAHKGKRSYRCSVHGHAAHSSCPHLGINSIDFAA--MMQLKIREI 226 Query: 247 ELAEKKSAVAGID----HATLNVGLIKGGINTNVVPDLASFRLDRRMIPEEAGFDAEGDL 302 L + S V D ++++ L GG N++PD A F + R +P G D ++ Sbjct: 227 ALRVRHSGVQDDDFDVPYSSIATTLTSGGNAPNIIPDKAEFVFEHRFLP---GIDP-AEV 282 Query: 303 RRVVDAAAAR--YPGIEA-GVQRILLAEPLAELPGV-----EKLIGAFSRNAEAILGEPV 354 V A A + P + GVQ + E L PG+ + L+ + R ILG Sbjct: 283 FEEVKAYAEQEILPQMRIDGVQGRIEFETLTSYPGMCTPADDPLVTSALR----ILGSE- 337 Query: 355 KAHGVPLYTDARHYTRQGVPTILYGAGPRTLMEARGHNSDENLRLNDLLKATK 407 +A V T+ + + G+P ++ G G A H +E + L L + + Sbjct: 338 RARKVGFGTEGGLFGQAGMPAVICGPGDI----AHAHKPNEFVTLEQLARCER 386 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 57.8 bits (138), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 68/280 (24%), Positives = 106/280 (37%), Gaps = 52/280 (18%) Query: 50 LEQLGFDVEVHVVPDELVKANGMISAANLIVRKRFGSGGPTIALNAHGDVVP----PGLG 105 LEQ G+ V H+ NG+++ R GS +I L A D +P GL Sbjct: 50 LEQWGYAVTRHI------GGNGLVATL------RVGSSARSIGLRADMDALPIQEETGLD 97 Query: 106 WSKDPYGGEIVQDAEHGPVMYGRGVAVSKSDFATYTWALLALIAAEKNGAALNGAIELQF 165 W+ G A+ +T LL +G + L F Sbjct: 98 WASVKPG------------------AMHACGHDGHTAMLLGAARHLARTRRFDGTLNLIF 139 Query: 166 THDEEAGGDIGPKWILEQGLSK--PDYAV----------SAGFAYGITSAHNGCLHLEVT 213 EEAG D G + +L GL + P AV + F + C +++ Sbjct: 140 QPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIR 199 Query: 214 VKGKQAHAAMPHTGIDAIEAATGILQALYAYRAELAEKKSAVAGIDHATLNVGLIKGGIN 273 + G+ +HAA PH +D + A ++ AL + + +D A + VG + G Sbjct: 200 ITGRGSHAARPHLSVDPVVVAASLVMALQTVVSR------NIDPMDSAVVTVGSLHAGKA 253 Query: 274 TNVVPDLASFRLDRRMIPEEAGFDAEGDLRRVVDAAAARY 313 +NV+P+ A+ L R E E +R +V A Y Sbjct: 254 SNVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSY 293 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,430,340 Number of extensions: 65195 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 5 Length of query: 418 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 330 Effective length of database: 1,203,190 Effective search space: 397052700 Effective search space used: 397052700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)