BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0607.p (190 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidogl... 196 1e-51 gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidogl... 105 2e-24 >gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 822 Score = 196 bits (497), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 99/136 (72%), Positives = 105/136 (77%), Gaps = 14/136 (10%) Query: 47 LAGKTGTTNDAFDGWFAGYQRTLATVVWMGYDQPKSLGSKEFGAQLALPIWVDYMGVALR 106 LAGKTGTTNDA DGWFAGYQRTLATVVWMGYDQPKSLGSKEFGAQLALPIWVDYM AL+ Sbjct: 668 LAGKTGTTNDAVDGWFAGYQRTLATVVWMGYDQPKSLGSKEFGAQLALPIWVDYMAKALK 727 Query: 107 GTPQSDTPAPAGIRFIDGEPYYENFTPGNGFVASLGVDGGGVVDALSNFFGWSKNPNSET 166 G PQ D P P GIRFIDGEPYY+NFTP NG V ++GV VVDA+SNFFGW+ Sbjct: 728 GVPQFDLPVPEGIRFIDGEPYYDNFTPNNGLVTNVGVT---VVDAISNFFGWT------- 777 Query: 167 QAAPSSGPA-PSSQAP 181 PS PA P S P Sbjct: 778 ---PSQAPATPGSTQP 790 Score = 82.8 bits (203), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 38/47 (80%), Positives = 43/47 (91%) Query: 1 VSYPAGNGQPNWEPKDDDPPAGPMAMRTALQKSVNLVAIRTLDAIGL 47 VSYPAG GQPNWEP+DD+ PAGP+ +RTALQKSVNLVAIRTLD IG+ Sbjct: 508 VSYPAGPGQPNWEPRDDETPAGPITVRTALQKSVNLVAIRTLDYIGV 554 >gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 771 Score = 105 bits (263), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 54/107 (50%), Positives = 65/107 (60%), Gaps = 1/107 (0%) Query: 37 VAIRTLDAIGLAGKTGTTNDAFDGWFAGYQRTLATVVWMGYDQPKSLGSKEFGAQLALPI 96 V +L LAGKTGTTND+ D WFAGYQ L + W+GYDQPKSLG +E G LALP+ Sbjct: 644 VKALSLKRTDLAGKTGTTNDSMDAWFAGYQPGLTAIAWIGYDQPKSLGDRETGGGLALPV 703 Query: 97 WVDYMGVALRGTPQSDTPAPAGIRFIDGEPYYENFTPGNGFVASLGV 143 W+ YM AL+ P D P G+ GE YY F P + V LG+ Sbjct: 704 WISYMSKALKDVPNVDRKVPDGLIESGGEYYYAEFPP-SAMVRDLGM 749 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.134 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 614,857 Number of extensions: 25807 Number of successful extensions: 63 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 63 Number of HSP's successfully gapped: 3 Length of query: 190 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 110 Effective length of database: 1,241,070 Effective search space: 136517700 Effective search space used: 136517700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 128 (53.9 bits)