BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0584.p (222 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 105 3e-24 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 105 4e-24 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 102 3e-23 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 98 5e-22 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 94 1e-20 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 105 bits (262), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 73/205 (35%), Positives = 104/205 (50%), Gaps = 18/205 (8%) Query: 1 LITGGANGIGKAAALSFVREGARVIIADIDKEAAQRVQETAAQIVRDANGEIRGGALAVH 60 LITGG +GIG+A AL++ REGAR+ I +D+E E A Q +D + L + Sbjct: 129 LITGGDSGIGRAVALAYAREGARLAIVYLDEE------EDARQTQKDVQA-LDAPCLLLP 181 Query: 61 CDVTDEASVRSAVETAVAHYGKLDVLYNNAGGSTTRDGTVTEAPVDEFWRAIRLDLFGTF 120 DV RSAV + YG+LDVL NNA ++ E + R ++F F Sbjct: 182 GDVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQ-SIEEISQAQLEHTFRTNIFSMF 240 Query: 121 LCCKIALPELIKAGGGIVINTTSIAAIKAFPGHDCYTAAKGGVSALTRSMAVEYAAHKIR 180 K A+P L + G +INTTS+ A + Y A KG + A TRS++ + A+ I Sbjct: 241 YMVKEAMPFLTR--GARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIH 298 Query: 181 VNALAPAVTLSRRKSRIWRSILPAT 205 VNA+AP IW ++PA+ Sbjct: 299 VNAVAPGP--------IWTPLIPAS 315 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 105 bits (261), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 72/187 (38%), Positives = 105/187 (56%), Gaps = 11/187 (5%) Query: 1 LITGGANGIGKAAALSFVREGARVIIADIDKEAAQRVQETAAQIVRDANGEIRGGALAVH 60 LITG A+GIGK A+ + R+GA+V+IAD+ EAA+ TA +IV++ G A+AV Sbjct: 9 LITGSASGIGKEIAVEYARQGAKVVIADLALEAAKA---TAEEIVKEG-----GTAMAVA 60 Query: 61 CDVTDEASVRSAVETAVAHYGKLDVLYNNAGGSTTRDGTVTEAPVDEFWRAIRLDLFGTF 120 +VTDE V V AV YG LD++ +NAG V E ++ + + + + L G F Sbjct: 61 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIIS--PVAELSLENWRKMLAIHLDGAF 118 Query: 121 LCCKIALPELIKAG-GGIVINTTSIAAIKAFPGHDCYTAAKGGVSALTRSMAVEYAAHKI 179 L + A+ +IK G GG +I S+ + A P Y AK G+ L +++A E A KI Sbjct: 119 LTTRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKI 178 Query: 180 RVNALAP 186 R N + P Sbjct: 179 RTNVICP 185 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 102 bits (254), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 63/176 (35%), Positives = 94/176 (53%), Gaps = 9/176 (5%) Query: 14 ALSFVREGARVIIADIDKEAAQRVQETAAQIVRDANGEIRGGALAVHCDVTDEASVRSAV 73 A ++ REGA +++ADI + RV E ++ A G A+ DV+ A + + Sbjct: 22 AKAYAREGAAIVVADIGEAGGLRVVEE----IKAAGGR----AVFAKADVSKRADMDQLL 73 Query: 74 ETAVAHYGKLDVLYNNAGGSTTRDGTVTEAPVDEFWRAIRLDLFGTFLCCKIALPELIKA 133 TA+ H+GKLD++ NNAG +T R+ + E DEF R +++ FL K +P + Sbjct: 74 ATALEHFGKLDIVVNNAG-TTHRNRPMLEVEEDEFDRVYAVNVKSIFLSAKTFVPYFRQV 132 Query: 134 GGGIVINTTSIAAIKAFPGHDCYTAAKGGVSALTRSMAVEYAAHKIRVNALAPAVT 189 GGG IN S A I+ PG Y +KG V ++SMA E KIRVN + P ++ Sbjct: 133 GGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRVNCVNPVIS 188 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 98.2 bits (243), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 68/189 (35%), Positives = 104/189 (55%), Gaps = 13/189 (6%) Query: 1 LITGGANGIGKAAALSFVREGARVIIADIDKEAAQRVQETAAQIVRDANGEIRGGALAVH 60 ++TG A+GIG+A A F EGARV++AD D A +V ++ R A A Sbjct: 18 IVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPP-ERTA---------ARE 67 Query: 61 CDVTDEASVRSAVETAVAHYGKLDVLYNNAGGSTTRDGTVTEAPVDEFWRAIRLDLFGTF 120 DV+ VR V+ A+ +G+LDVL ++AG +G VTE +++ R ++ G F Sbjct: 68 TDVSHHEQVRQLVDFAIERFGQLDVLVSDAG--VFAEGNVTEVSPEDWHRVQATNVNGVF 125 Query: 121 LCCKIALPELIKAGGGIVINTTSIAAIKAFPGHDCYTAAKGGVSALTRSMAVEYAAHKIR 180 + ALP L K G IV N S++ + A Y A+KG V LTR+MA+++ +R Sbjct: 126 YGAREALPHLEKTRGCIV-NVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVR 184 Query: 181 VNALAPAVT 189 +NA+ P++T Sbjct: 185 INAVCPSLT 193 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 94.0 bits (232), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 70/203 (34%), Positives = 101/203 (49%), Gaps = 22/203 (10%) Query: 1 LITGGANGIGKAAALSFVREGARVIIADIDKEAAQRVQETAAQIVRDANGEIRGGALAVH 60 ++T GA GIG A +FV GA V I D+ ++ A+ R G V Sbjct: 11 IVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFL-------------ASARARFGHAPVS 57 Query: 61 CDVTDEASVRSA----VETAVAHYGKLDVLYNNAG--GSTTRDGTVTEAPVDEFWRAIRL 114 TD +S R + A G+LDVL NNAG G T+ V E + ++ + + + Sbjct: 58 YSRTDVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTS---PVEEVALSDWDQTLAV 114 Query: 115 DLFGTFLCCKIALPELIKAGGGIVINTTSIAAIKAFPGHDCYTAAKGGVSALTRSMAVEY 174 +L G FLC + A+P L K GGG ++N +S+A F Y+A+K GV LT + A+E Sbjct: 115 NLTGPFLCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIEL 174 Query: 175 AAHKIRVNALAPAVTLSRRKSRI 197 IRVNA+ P + R+ RI Sbjct: 175 GPSHIRVNAVLPGIVEGARQERI 197 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 702,929 Number of extensions: 28257 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 44 Length of query: 222 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 140 Effective length of database: 1,231,600 Effective search space: 172424000 Effective search space used: 172424000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)