BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0002.0579.p
         (145 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas...   207   3e-55
gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen...    78   3e-16
gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen...    69   2e-13

>gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 298

 Score =  207 bits (527), Expect = 3e-55,   Method: Compositional matrix adjust.
 Identities = 104/126 (82%), Positives = 114/126 (90%)

Query: 1   MSKNVGVIGLGAMGLGVARSLLRAGFKVHACDVRKEVLDKFAADGGIACASPAEMGKAVD 60
           MS+NVGVIGLGAMG GVA SLLRAGF VHACDVRKEVLDKFAA GG+ACA+PAE+   VD
Sbjct: 1   MSRNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVD 60

Query: 61  VIITLVVNAAQTDTVLFGENGAASTLKPGSVVISSATVAPDFAVALGQRLAEKDLLLLDA 120
           V+ITLVVNAAQT+TVLFG+NGA S +KPGSVVISSATVAPDFA+ LG+RL EK LLLLDA
Sbjct: 61  VVITLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDA 120

Query: 121 PVSGGA 126
           PVSGGA
Sbjct: 121 PVSGGA 126


>gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 297

 Score = 78.2 bits (191), Expect = 3e-16,   Method: Compositional matrix adjust.
 Identities = 50/128 (39%), Positives = 77/128 (60%), Gaps = 2/128 (1%)

Query: 1   MSKNVGVIGLGAMGLGVARSLLRAGFKVHACDVRKEVLDKFAADGGIACA-SPAEMGKAV 59
           MS N+  IGLG MGL +A++L++AGF V   D+ K  +DK    GG   A S A + KA 
Sbjct: 1   MSANIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKA- 59

Query: 60  DVIITLVVNAAQTDTVLFGENGAASTLKPGSVVISSATVAPDFAVALGQRLAEKDLLLLD 119
           DV+IT++  +   +++  GE G  ++ KPG+++I  +T+AP+ A  +      +   +LD
Sbjct: 60  DVVITMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLD 119

Query: 120 APVSGGAK 127
           APVSGG  
Sbjct: 120 APVSGGTN 127


>gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 288

 Score = 68.9 bits (167), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 44/125 (35%), Positives = 65/125 (52%)

Query: 3   KNVGVIGLGAMGLGVARSLLRAGFKVHACDVRKEVLDKFAADGGIACASPAEMGKAVDVI 62
           K +GVIGLG MG G+A SL R G++V   D ++      A  G     S A +  AV+++
Sbjct: 2   KKIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEMV 61

Query: 63  ITLVVNAAQTDTVLFGENGAASTLKPGSVVISSATVAPDFAVALGQRLAEKDLLLLDAPV 122
           +  +  +   + V+ GE G      PG V++ + T  P+    L  RL    + L+DAPV
Sbjct: 62  VLSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAPV 121

Query: 123 SGGAK 127
           SGG K
Sbjct: 122 SGGPK 126


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 502,607
Number of extensions: 20526
Number of successful extensions: 137
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 136
Number of HSP's successfully gapped: 4
Length of query: 145
Length of database: 1,619,870
Length adjustment: 77
Effective length of query: 68
Effective length of database: 1,255,275
Effective search space: 85358700
Effective search space used: 85358700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 126 (53.1 bits)