BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0002.0578.p
         (162 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas...   323   3e-90
gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen...    83   1e-17

>gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 298

 Score =  323 bits (829), Expect = 3e-90,   Method: Compositional matrix adjust.
 Identities = 156/161 (96%), Positives = 159/161 (98%)

Query: 1   MMTSGPAEVYARIEDVLKGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAASAEAMALGL 60
           MMTSGPAEVYA+IEDVL GMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAASAEAMALGL
Sbjct: 136 MMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAASAEAMALGL 195

Query: 61  REGVDPDALYEVITHSAGNSWMFENRVPHILKGDYTPLSAVDIFVKDLGLVLDTARRSKF 120
           REGV+PDALYEVITHSAGNSWMFENRVPHIL GDYTPLSAVDIFVKDLGLVLDTARRSKF
Sbjct: 196 REGVNPDALYEVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFVKDLGLVLDTARRSKF 255

Query: 121 PLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPPSK 161
           PLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPP+K
Sbjct: 256 PLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPPAK 296


>gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 297

 Score = 83.2 bits (204), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 47/158 (29%), Positives = 82/158 (51%), Gaps = 8/158 (5%)

Query: 2   MTSGPAEVYARIEDVLKGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAASAEAMALGLR 61
           M  G  E +   +  L+ M   +Y  G++ G G  VK+ N +L G+ +  ++EA+ LG+ 
Sbjct: 137 MVGGSKEAFEAAQPYLQKMGKAIYHAGES-GSGQTVKVCNNMLLGILMIGTSEAIRLGMA 195

Query: 62  EGVDPDALYEVITHSAGNSWMFE--NRVPHILKG-----DYTPLSAVDIFVKDLGLVLDT 114
            G+DP  L E+++ S+G +W  E  N  P +++       Y     VD+ +KDLGL ++ 
Sbjct: 196 NGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGFGVDLMLKDLGLAVEN 255

Query: 115 ARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIF 152
           A  +   +PL A A  ++ + S  G G  D S++  + 
Sbjct: 256 ALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNML 293


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 541,575
Number of extensions: 21456
Number of successful extensions: 57
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 58
Number of HSP's successfully gapped: 3
Length of query: 162
Length of database: 1,619,870
Length adjustment: 78
Effective length of query: 84
Effective length of database: 1,250,540
Effective search space: 105045360
Effective search space used: 105045360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 127 (53.5 bits)