BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0575.p (441 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 711 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 531 e-152 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 395 e-111 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 357 1e-99 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 348 7e-97 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 711 bits (1836), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/441 (80%), Positives = 389/441 (88%), Gaps = 4/441 (0%) Query: 1 MTASTNIGAAPAVNTSANEQEQNRVYAKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSV 60 MT ++ G V N++ ++ +Y KVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMS Sbjct: 1 MTTASTTG----VGLDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQ 56 Query: 61 DLGFSETVFGLGAGVFFLGYFLFEVPSNILMHKVGARVWIARIMITWGILSAAFMYVETP 120 DLGFSETVFGLGAG+FF+GYFLFEVPSNILMHKVGAR+WIARIMITWGILSAAFM+V+ Sbjct: 57 DLGFSETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNA 116 Query: 121 MQFYVLRFLLGLAEAGFYPGIILYLTYWYPSHRRAKVIAVFMSGIPVAGILGNPLSGWIM 180 QFY+LRFLLGLAEAGFYPGIILYLTYW+PSHRRAKVIAVFMSGIPVAGILGNPLSGWIM Sbjct: 117 TQFYILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIM 176 Query: 181 DAFHGTTGMHGWQWMFVIEAVPAVLIGLATLAYLDNGIAKAKWLSAGEKKLLEDEIAADQ 240 DAFH G+ GWQWMF+IEA+PAVLIG+AT+ YLDN + AKWL+ EK L+ +I D Sbjct: 177 DAFHQNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDA 236 Query: 241 KGKESVHSFADIVSDKRVWLMCLIYFCFVMGQYGLTLWMPTLVKATGVTGNLHIGLLSAI 300 KGKES H F IV D RVWLMCLIYF FVMGQYGLTLWMPTLVKATGV GNL IGLLSAI Sbjct: 237 KGKESKHGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAI 296 Query: 301 PFGCAIIAMNLIGRSADARRERRWHLIVPALMGAVGFVGAALFADNTAISIAALSLAAAG 360 PFGCAIIAMNLIGRSAD RERRWHL++PALMG VGFVGAALFADNTA+SIA+LSLAAAG Sbjct: 297 PFGCAIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAG 356 Query: 361 VLTCAPLFWSLPTSFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHSGATGMYML 420 VLTCAPLFWSLPT+FLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTH+ ATGMYML Sbjct: 357 VLTCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYML 416 Query: 421 AIMLVIGSAAVLKTSPKLVNK 441 A+MLV+GS A L T P +VNK Sbjct: 417 AVMLVVGSIATLATKPGMVNK 437 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 531 bits (1367), Expect = e-152, Method: Compositional matrix adjust. Identities = 251/420 (59%), Positives = 329/420 (78%), Gaps = 1/420 (0%) Query: 22 QNRVYAKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSVDLGFSETVFGLGAGVFFLGYF 81 ++ Y+KV WR++P L LCYV +YLDRVNVGFAKLQM DL FS+TV+GLGAG+FFLGYF Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 82 LFEVPSNILMHKVGARVWIARIMITWGILSAAFMYVETPMQFYVLRFLLGLAEAGFYPGI 141 +FEVPSN+++HKVGAR+WIARIMITWGI+S A YV TP FY++RFLLG+AEAGF+PG+ Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 142 ILYLTYWYPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHGTTGMHGWQWMFVIEAV 201 ILYLTYWYP+ RR K+ A+FM+GI ++G++G PLSGWIM A G G GWQWMF++EA+ Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 202 PAVLIGLATLAYLDNGIAKAKWLSAGEKKLLEDEIAADQKGKESVHSFADIVSDKRVWLM 261 P++++G+ + Y+ + I A WLS EK+LLE I+ ++ KE + S + ++ RVWL Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEHL-SLGAMFANGRVWLG 255 Query: 262 CLIYFCFVMGQYGLTLWMPTLVKATGVTGNLHIGLLSAIPFGCAIIAMNLIGRSADARRE 321 LIYFCFVMG YG++ W+PT++KATGV+ L++GLL+AIP+ A AM LIGRSADARRE Sbjct: 256 ALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRE 315 Query: 322 RRWHLIVPALMGAVGFVGAALFADNTAISIAALSLAAAGVLTCAPLFWSLPTSFLSGAAA 381 RRWH+ +PA +G +G + + + NT +++A+L+LA+ G+LT PLFWSLPT+FLSG AA Sbjct: 316 RRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAA 375 Query: 382 AVGIAAINSVGNLAGFVSPYLIGYLKDLTHSGATGMYMLAIMLVIGSAAVLKTSPKLVNK 441 A GIA INS+GNLAGFVSP+L+G+LKD T S GMY+LA LVIG L KLVN+ Sbjct: 376 AAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTLPAKLVNR 435 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 395 bits (1016), Expect = e-111, Method: Compositional matrix adjust. Identities = 198/403 (49%), Positives = 275/403 (68%) Query: 26 YAKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSVDLGFSETVFGLGAGVFFLGYFLFEV 85 Y K+ WR++PFL+ +V+A++DRVNVGFAKLQM DL FSE V+GLGAG+FF+GYF+FEV Sbjct: 25 YRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEV 84 Query: 86 PSNILMHKVGARVWIARIMITWGILSAAFMYVETPMQFYVLRFLLGLAEAGFYPGIILYL 145 PSN+L+ K+GAR +ARI I WG+ S A YV TP FY+LRFLLG+ EAGF+PG++LYL Sbjct: 85 PSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYL 144 Query: 146 TYWYPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHGTTGMHGWQWMFVIEAVPAVL 205 TYW+P+ RRA+V +FM+ +AG +G P++G IM G + WQW+F++E +P++L Sbjct: 145 TYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSIL 204 Query: 206 IGLATLAYLDNGIAKAKWLSAGEKKLLEDEIAADQKGKESVHSFADIVSDKRVWLMCLIY 265 G A LA+L A AKWLS EK + +AA+ SF D ++ RVW+ +Y Sbjct: 205 AGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICAAVY 264 Query: 266 FCFVMGQYGLTLWMPTLVKATGVTGNLHIGLLSAIPFGCAIIAMNLIGRSADARRERRWH 325 FC V G + W P+++K GV GNL IGL+SAIPF +AM G +D ERR H Sbjct: 265 FCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERRLH 324 Query: 326 LIVPALMGAVGFVGAALFADNTAISIAALSLAAAGVLTCAPLFWSLPTSFLSGAAAAVGI 385 + L+ +G V + N +++ +L+LAA G+L P+FWS+P +FL+G AAA GI Sbjct: 325 SAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAGGI 384 Query: 386 AAINSVGNLAGFVSPYLIGYLKDLTHSGATGMYMLAIMLVIGS 428 A INS+GNLAGFV+PY+IG LK T S ++G+Y +A + ++ S Sbjct: 385 ALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVAS 427 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 357 bits (915), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 196/417 (47%), Positives = 267/417 (64%), Gaps = 11/417 (2%) Query: 14 NTSANEQEQ-NRVYAKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSVDLGFSETVFGLG 72 N S Q + +Y KV W+I+P+L+L YVIAYLDRVNVGFAKL M DLG SE +GLG Sbjct: 3 NASQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLG 62 Query: 73 AGVFFLGYFLFEVPSNILMHKVGARVWIARIMITWGILS---------AAFMYVETPMQF 123 AG+FFLGYF+FEVPSN+++H+VGA++WIARIMITWG++S A ++ V F Sbjct: 63 AGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVF 122 Query: 124 YVLRFLLGLAEAGFYPGIILYLTYWYPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAF 183 Y LRFLLG +EAGF+PG+ILY+TYWYP+ ++V+AV + G P + ILG P+SG +M Sbjct: 123 YTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFM 182 Query: 184 HGTTGMHGWQWMFVIEAVPAVLIGLATLAYLDNGIAKAKWLSAGEKKLLEDEIAADQKGK 243 + GWQWMFVIEA+PA L+G+ L L N IA AKWL+ EK L +AA+ K K Sbjct: 183 GSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEK 242 Query: 244 ESVHSFADIVSDKRVWLMCLIYFCFVMGQYGLTLWMPTLVKATGVTGNLHIGLLSAIPFG 303 +V + + +W + L+Y ++G YG+ W PT+++ GV L IG++SAIP+ Sbjct: 243 VNV-PLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYI 301 Query: 304 CAIIAMNLIGRSADARRERRWHLIVPALMGAVGFVGAALFADNTAISIAALSLAAAGVLT 363 AM I R A+ + R + + +++ + V LF N I++A +++A G LT Sbjct: 302 LGATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLT 361 Query: 364 CAPLFWSLPTSFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHSGATGMYML 420 LFW P ++ +GA AA GIA INSVGNL GFV PYL+G L S G+ ML Sbjct: 362 SIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMML 418 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 348 bits (892), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 183/415 (44%), Positives = 256/415 (61%), Gaps = 6/415 (1%) Query: 1 MTASTNIGAAPAVNTSANEQEQNRVYAKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSV 60 MT S+ GA + S Q ++ +Y KV+ RI+PFL +CYV+++LDR+N+GFA+LQM Sbjct: 1 MTPSSG-GATLSTTPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQ 59 Query: 61 DLGFSETVFGLGAGVFFLGYFLFEVPSNILMHKVGARVWIARIMITWGILSAAFMYVETP 120 DLGFS+ ++GLGA +F++GY LFEVPSN+ + + GAR +RIM+ WG+ S M V Sbjct: 60 DLGFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDA 119 Query: 121 MQFYVLRFLLGLAEAGFYPGIILYLTYWYPSHRRAKVIAVFMSGIPVAGILGNPLSGWIM 180 FY LRFLLG+ EAGF+PGI+LYL+YW+P+ RRA V+A+F +G+ VAG+LG +SGWIM Sbjct: 120 AHFYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIM 179 Query: 181 DAFHGTTGMHGWQWMFVIEAVPAVLIGLATLAYLDNGIAKAKWLSAGEKKLLE--DEIAA 238 G G++GWQWMF IE PAV++GL + L +G A+AKWLS E+ L+ + A Sbjct: 180 RDMAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEA 239 Query: 239 DQKGKESVHSFADI---VSDKRVWLMCLIYFCFVMGQYGLTLWMPTLVKATGVTGNLHIG 295 + S HS A + +S + +L IYF G L WMP +++ G+ + + Sbjct: 240 LRTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVS 299 Query: 296 LLSAIPFGCAIIAMNLIGRSADARRERRWHLIVPALMGAVGFVGAALFADNTAISIAALS 355 L S IP + + LI R +D R ER H V GA+ L + A+S+ LS Sbjct: 300 LYSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLS 359 Query: 356 LAAAGVLTCAPLFWSLPTSFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLT 410 +AA + P+FWSLP L A GIA I+SVG +G VSP+LIG +K T Sbjct: 360 VAAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRT 414 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,235,075 Number of extensions: 48274 Number of successful extensions: 315 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 280 Number of HSP's successfully gapped: 20 Length of query: 441 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 353 Effective length of database: 1,203,190 Effective search space: 424726070 Effective search space used: 424726070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)