BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0469.p (344 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 573 e-165 gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 259 3e-70 gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 244 1e-65 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 89 5e-19 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 573 bits (1477), Expect = e-165, Method: Compositional matrix adjust. Identities = 293/342 (85%), Positives = 317/342 (92%) Query: 1 MQEAASGTPTPNSYRDAARQAMPSVVNIFTTTEARPQKNPFMDDPFFKKFFGDQFEDQQQ 60 MQEA P +SYR AARQAMPSVVNIFTTTEARPQK+PF +DPFF+KFFGDQF++QQQ Sbjct: 47 MQEATPNAPALDSYRHAARQAMPSVVNIFTTTEARPQKSPFQNDPFFRKFFGDQFDEQQQ 106 Query: 61 DDKQSSLGSGVIVSPQGYILTNNHVVEAADEIEVALADGRKASAKVVGTDPETDLAVIKV 120 DDKQSSLGSGVIVSPQGYILTNNHVVEAAD+IEVALADGRKASAKVVG DPETDLAVIK+ Sbjct: 107 DDKQSSLGSGVIVSPQGYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKI 166 Query: 121 DLPNLPAITLAHVEQATVGDIVLAIGNPFGVGQTVTMGIISALGRNHLGINTFENFIQTD 180 DLPNLPAITL H E ++VGD+VLAIGNPFGVGQTVTMGI+SALGRNHLGINTFENFIQTD Sbjct: 167 DLPNLPAITLGHPENSSVGDVVLAIGNPFGVGQTVTMGIVSALGRNHLGINTFENFIQTD 226 Query: 181 AAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIPVSTAKTVMEAIINHGQVVR 240 AAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIP+STAKTVMEAII+HGQVVR Sbjct: 227 AAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIPMSTAKTVMEAIISHGQVVR 286 Query: 241 GWIGVEPQDITPELAESFGLTKKTGAIIAGVLKNGPADKAGMKPGDILISVENKAVTDTT 300 GWIGVEPQDITPELAESFGL KKTGAIIAGVLK GPAD+AGM+PGDIL+S+++K V DTT Sbjct: 287 GWIGVEPQDITPELAESFGLGKKTGAIIAGVLKGGPADRAGMRPGDILVSIDDKPVADTT 346 Query: 301 EMLNLIAQLIPGQKARMTVLRKAQETTLEIVVGKRPPLKREE 342 EMLN+IAQL PGQ+ MTVLRK+QET L I VGKRPP + E Sbjct: 347 EMLNVIAQLTPGQQIAMTVLRKSQETKLNITVGKRPPPPKNE 388 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 259 bits (661), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 144/328 (43%), Positives = 202/328 (61%), Gaps = 8/328 (2%) Query: 23 PSVVNIFTTTEARP---QKNPFMD--DPF--FKKFFGDQFEDQQQDDKQSSLGSGVIVSP 75 P+VVNI T +AR + +D DPF F K FG Q + QQ LGSG I+SP Sbjct: 74 PAVVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFGPQLQMPQQPRIMHGLGSGFIISP 133 Query: 76 QGYILTNNHVVEAADEIEVALADGRKASAKVVGTDPETDLAVIKVDLPNLPAITLAHVEQ 135 G ILTN HVV+ A E+ V L D R+ AKV+G D ++D+AVI++D NLP + + + Sbjct: 134 DGLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRIDAKNLPTVQIGDPSR 193 Query: 136 ATVGDIVLAIGNPFGVGQTVTMGIISALGRNHLGINTFENFIQTDAAINPGNSGGALVDT 195 VG VLAIG+P+G T T GIISA R+ L + + FIQTD A+NPGNSGG L D Sbjct: 194 VKVGQPVLAIGSPYGFDNTATAGIISAKSRS-LPDDNYVPFIQTDVAVNPGNSGGPLFDL 252 Query: 196 NGNLLGINTAIYSRTGGNLGIGFAIPVSTAKTVMEAIINHGQVVRGWIGVEPQDITPELA 255 NG ++GIN+ IYS+TGG G+ F+IP+ A V + ++ HG+V RG +GV QD+ L+ Sbjct: 253 NGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKVEQQLVTHGKVTRGRLGVSVQDLNQALS 312 Query: 256 ESFGLTKKTGAIIAGVLKNGPADKAGMKPGDILISVENKAVTDTTEMLNLIAQLIPGQKA 315 +SFGL K GA+++ V K PADKAG++ GD++IS A+ + ++ L+A PG Sbjct: 313 DSFGLKKAEGALVSSVEKGSPADKAGLQAGDVIISFNGHAIDHSVDLPTLVADTPPGSTK 372 Query: 316 RMTVLRKAQETTLEIVVGKRPPLKREER 343 M V+R + T+ + VG+ K + + Sbjct: 373 PMEVMRAGKVRTINVTVGEMKQAKNDAK 400 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 244 bits (622), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 135/341 (39%), Positives = 200/341 (58%), Gaps = 25/341 (7%) Query: 14 YRDAARQAMPSVVNIFTTTEAR--PQKNPFMDDP----FFKKFFGDQFEDQQQ------- 60 + D + P VVNI TT R Q +DDP FF++FFG QQQ Sbjct: 41 FADIVERTGPGVVNIRTTERVRQNAQGQGGVDDPEMQEFFRRFFGIPIPRQQQPGTPRGN 100 Query: 61 ---------DDKQSSLGSGVIVSPQGYILTNNHVVEAADEIEVALADGRKASAKVVGTDP 111 ++ +GSG I+S G+I+TN HVVE A E+ V L D R+ AK+VG+D Sbjct: 101 GKQGGQGQQEEVPRGVGSGFIISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDT 160 Query: 112 ETDLAVIKVDLPNLPAITLAHVEQATVGDIVLAIGNPFGVGQTVTMGIISALGRNHLGIN 171 TD+AV+K+D NLP + + ++ VG+ VLAIG+PFG+ TVT GI+SA R+ Sbjct: 161 RTDVAVLKIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARD---TG 217 Query: 172 TFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIPVSTAKTVMEA 231 + IQTD A+NPGNSGG L++ G ++GIN+ IYSR+GG +GI FA+P+ A V + Sbjct: 218 DYLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVADQ 277 Query: 232 IINHGQVVRGWIGVEPQDITPELAESFGLTKKTGAIIAGVLKNGPADKAGMKPGDILISV 291 + G+V RG IGV+ ++T ++AES GL + GA++ V GPA+KAG++ GDI++ Sbjct: 278 LKASGRVTRGRIGVQIGEVTKDVAESLGLARAQGALVQRVEAGGPAEKAGLEAGDIILKY 337 Query: 292 ENKAVTDTTEMLNLIAQLIPGQKARMTVLRKAQETTLEIVV 332 A+ +++ ++ PG KA +++ RK + + V Sbjct: 338 NGAAIERPSDLPRMVGSTKPGAKATVSIWRKGSARDVSVTV 378 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 89.0 bits (219), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 56/163 (34%), Positives = 95/163 (58%), Gaps = 15/163 (9%) Query: 68 GSGVIVSPQGYILTNNHVVEAADEIEVALADGRKASAKVVGTDPETDLAVIKVDLPNLPA 127 G+G + G++L+N HVV A + V L GR+ A+V+ D D+A++K + P + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 128 ITLAHVEQATVGDIVLAIGNPFG--VGQTVTMGIISALGRNHLGINTF--ENFIQTDAAI 183 + L +++ +G+ V A+G+P G T+T GI+S G+ + F+Q+D AI Sbjct: 331 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILS-------GVRLLNGQEFLQSDVAI 382 Query: 184 NPGNSGGALVDTNGNLLGINTA-IYSRTGGNLGIGFAIPVSTA 225 PG+SGG L+D +G ++GI A + +R G G+ F IP+++A Sbjct: 383 LPGSSGGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASA 423 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,035,075 Number of extensions: 43471 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 4 Length of query: 344 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 258 Effective length of database: 1,212,660 Effective search space: 312866280 Effective search space used: 312866280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)