BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0002.0445.p
         (310 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 compo...   572   e-164
gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 compo...   337   1e-93

>gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1
           component protein [Herbaspirillum seropedicae SmR1]
          Length = 893

 Score =  572 bits (1474), Expect = e-164,   Method: Compositional matrix adjust.
 Identities = 269/295 (91%), Positives = 285/295 (96%)

Query: 5   SASGIAKDIDIDAQETQEWLQALQGVLQEVGPDRARFLLSRMSAAAQQWGINWRDARNTP 64
           S S IA+DIDID+QETQEWLQALQ VL E GP+RARFLLSR+SAAAQQWG+NWRDARNTP
Sbjct: 5   SDSDIARDIDIDSQETQEWLQALQSVLAEAGPERARFLLSRLSAAAQQWGVNWRDARNTP 64

Query: 65  YINTIRPEQEPPFPGGSDAQAIEEHLASVMRWNALAMVVRANRAYGELGGHIASYASAAD 124
           Y+NTIRPEQEPPFPGGSDAQAIEE +AS+MRWNALAMVVRANRAYGELGGHIAS+ASAAD
Sbjct: 65  YVNTIRPEQEPPFPGGSDAQAIEERIASIMRWNALAMVVRANRAYGELGGHIASFASAAD 124

Query: 125 LFEVGFNHFFRARDAQFAGDLVYFQPHSAPGIYARAFLEGALTEDDLGFYRREIEAKKHG 184
           LFEVGFNHFFRARD QFAGD+VYFQPHSAPGIYARAFLEGAL+EDDL +YRREIEAK+ G
Sbjct: 125 LFEVGFNHFFRARDEQFAGDVVYFQPHSAPGIYARAFLEGALSEDDLAYYRREIEAKRVG 184

Query: 185 KQGLSSYPHPWLMPNFWQFPTGSMGIGPINAIYQARFLRYLEHRGLLQDQGRKVWGVFGD 244
           +QGLSSYPHPWLMP FWQFPTGSMGIGPINAIYQARFLRY+EHRGLLQDQGRKVWGVFGD
Sbjct: 185 RQGLSSYPHPWLMPQFWQFPTGSMGIGPINAIYQARFLRYMEHRGLLQDQGRKVWGVFGD 244

Query: 245 GEMDEPESLAALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELETIFAGA 299
           GEMDEPESLAALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELET+FAGA
Sbjct: 245 GEMDEPESLAALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELETLFAGA 299


>gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1
           component protein [Herbaspirillum seropedicae SmR1]
          Length = 898

 Score =  337 bits (863), Expect = 1e-93,   Method: Compositional matrix adjust.
 Identities = 167/286 (58%), Positives = 206/286 (72%), Gaps = 8/286 (2%)

Query: 14  DIDAQETQEWLQALQGVLQEVGPDRARFLLSRMSAAAQQWGINWRDARNTPYINTIRPEQ 73
           D D  ETQEWL AL+ V+++ GPDRA +L+ RM   A++ G +   + NT Y+NTI  +Q
Sbjct: 15  DPDVMETQEWLDALEAVIEKEGPDRAHYLMERMVDLARRRGAHIPFSSNTAYVNTIPADQ 74

Query: 74  EPPFPGGSDAQAIEEHLASVMRWNALAMVVRANRAYGELGGHIASYASAADLFEVGFNHF 133
               PG  +    EE L S MRWNA+AMVV+ANR  G+LGGH++S+AS A++  +GFNHF
Sbjct: 75  GEHCPGNLE---YEERLRSWMRWNAMAMVVKANRVDGDLGGHLSSFASLANMLGIGFNHF 131

Query: 134 FRARDAQFAGDLVYFQPHSAPGIYARAFLEGALTEDDLGFYRREIEAKKHGKQGLSSYPH 193
           + A      GDL+Y Q HS+PGIYARAFLEG LTED L  +RRE +       GLSSYPH
Sbjct: 132 WHAPTEDHGGDLLYIQGHSSPGIYARAFLEGRLTEDQLIHFRREADG-----HGLSSYPH 186

Query: 194 PWLMPNFWQFPTGSMGIGPINAIYQARFLRYLEHRGLLQDQGRKVWGVFGDGEMDEPESL 253
           P LMP FWQFPT SMG+GP+ AIYQARFL+YL  RG+ +   RKVW   GDGEMDEPES+
Sbjct: 187 PKLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEMDEPESM 246

Query: 254 AALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELETIFAGA 299
            A+ +A REKL+NL+ VVNCNLQRLDGPVRGNG I+ ELE+ F GA
Sbjct: 247 GAIGMAGREKLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGA 292


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.137    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,026,145
Number of extensions: 44157
Number of successful extensions: 118
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 117
Number of HSP's successfully gapped: 2
Length of query: 310
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 225
Effective length of database: 1,217,395
Effective search space: 273913875
Effective search space used: 273913875
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 130 (54.7 bits)