BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0444.p (525 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 compo... 830 0.0 gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 compo... 530 e-152 >gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 830 bits (2143), Expect = 0.0, Method: Compositional matrix adjust. Identities = 410/525 (78%), Positives = 461/525 (87%), Gaps = 11/525 (2%) Query: 1 MKKIHAAYHAAASHRGQPTVILAQTKKGYGMGAAGEGKMTTHQQKKLDEEVLIAFRNRFK 60 MKKI++AY AAA HRGQPTVILAQTKKGYGMGAAGEGKMTTHQQKKLDEE L+ FR+RFK Sbjct: 380 MKKIYSAYQAAARHRGQPTVILAQTKKGYGMGAAGEGKMTTHQQKKLDEESLLQFRDRFK 439 Query: 61 LPISDEQCKSLAFYKPAADSAEMKHLLARRASLGGRMPRRVANADDKAQLRVPPIETHAR 120 LP+SDEQC++LAFYKPA DS EMKHL ARRA+L G +PRR A +A+ VPP+ H++ Sbjct: 440 LPLSDEQCRALAFYKPADDSPEMKHLHARRAALSGYLPRRQAG---QARRAVPPLAAHSK 496 Query: 121 FALDAEGKEMSSTMALVRMLGSMLKDADLGRYVVPIVADEARTFGMANLFRQVGIYSSQG 180 FAL+A+GKEMSSTMALVR LG++LKD D G+YVVPIVADEARTFGMANLFRQVGIYSSQG Sbjct: 497 FALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADEARTFGMANLFRQVGIYSSQG 556 Query: 181 QLYEPEDIGSILSYREAKDGQILEEGITEAGAISSWTAAATSYSVHGLPMLPFYIYYSMF 240 QLYEPEDIGSIL YREAKDGQILEEGITEAGAISSWTAAAT+YSVHG PMLPFYIYYSMF Sbjct: 557 QLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAATAYSVHGTPMLPFYIYYSMF 616 Query: 241 GFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGSSHVTAAGIPNCVSYDPAY 300 GFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDG+S VTAA IPNCVSYDPAY Sbjct: 617 GFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGNSLVTAASIPNCVSYDPAY 676 Query: 301 AYEMAVIVDDGMRRMLERNEDVFYYVTVTNENEEQPSLPQGRDVKDGILRGMYCLAPQAD 360 AYE+AVI+D+GMRRM+ER +DVFYY+TVTNENE QPSLP+G V+DGILRG+YCLA + Sbjct: 677 AYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPEG--VQDGILRGLYCLARKPQ 734 Query: 361 AQVRLLAAGPMLKEALSAARMLQEQFGVTAEVWSVTSFTELARDGIAAERRARLQPGAKG 420 AQ RLL +GP+LKEA++AA +LQ+ + + AEVWSVTS+TELARDG+AA+R RL Sbjct: 735 AQARLLGSGPILKEAVAAAALLQQHWQIEAEVWSVTSYTELARDGVAAQRAQRL--AGSA 792 Query: 421 ALPYVTQQLTQQLQSGQAPVIAVSDYVRTLSESIRAYVPARYVTLGTDGFGRSDTRANLR 480 ALPYV TQQLQ PVIAVSDY+RTL ESIRA+VPA YVTLGTDGFGRSDTR NLR Sbjct: 793 ALPYV----TQQLQGSAVPVIAVSDYIRTLPESIRAFVPAPYVTLGTDGFGRSDTRVNLR 848 Query: 481 DFFEVDARWIAYTALAEVFGADAARLKDAAAKLGVDLDKPLSTSV 525 DFFE+DARWIAYTAL EV+G D ARL++AA LG+D KPLS++V Sbjct: 849 DFFEIDARWIAYTALCEVWGDDRARLQEAARTLGIDTAKPLSSTV 893 >gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 898 Score = 530 bits (1366), Expect = e-152, Method: Compositional matrix adjust. Identities = 271/529 (51%), Positives = 360/529 (68%), Gaps = 21/529 (3%) Query: 3 KIHAAYHAAASHRGQPTVILAQTKKGYGMGAAGEGKMTTHQQKKLDEEVLIAFRNRFKLP 62 KI+AA+ A + QPTVILA++ KGYG G AGE + T H KKLD+E + A R+RF+LP Sbjct: 375 KIYAAFKVAQESKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLDDEAIRAMRDRFQLP 434 Query: 63 ISDEQCKSLAFYKPAADSAEMKHLLARRASLGGRMPRRVANADDKAQLRVPPIET-HARF 121 ISD ++ F+KP+ D+ EM++L RR +LGG +P+R AD+K L VP + A Sbjct: 435 ISDADLPNIPFFKPSDDAPEMQYLHERRKALGGYLPQRRVQADEK--LPVPELSAFQAML 492 Query: 122 ALDAEGKEMSSTMALVRMLGSMLKDADLGRYVVPIVADEARTFGMANLFRQVGIYSSQGQ 181 AEG+E+S+T A R+L ++L+D +LG VVPI+ DE+RTFGM LFRQ+GI+S GQ Sbjct: 493 EPTAEGREISTTAAYSRVLTALLRDPNLGPRVVPIMVDESRTFGMEGLFRQIGIFSQVGQ 552 Query: 182 LYEPEDIGSILSYREAKDGQILEEGITEAGAISSWTAAATSYSVHGLPMLPFYIYYSMFG 241 LYEP D ++ YRE K GQIL+EGI EAG +SSW AAATSYS + M+PFY YYSMFG Sbjct: 553 LYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRVMIPFYTYYSMFG 612 Query: 242 FQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGSSHVTAAGIPNCVSYDPAYA 301 QRIGDL WAA D RARGFLIG T+GRTTL GEGLQH+DG SHV A+ IPNC+ YDP +A Sbjct: 613 LQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHEDGHSHVFASAIPNCIPYDPTFA 672 Query: 302 YEMAVIVDDGMRRMLERNEDVFYYVTVTNENEEQPSLPQGRDVKDGILRGMYCL---APQ 358 +E+AVI+ DG+RRM+ EDVFYY+TV NEN P + G++ +GI++G+Y L Q Sbjct: 673 HEVAVIIHDGLRRMVANQEDVFYYITVMNENYGHPGIKPGQE--EGIIKGLYLLNEGGKQ 730 Query: 359 ADAQVRLLAAGPMLKEALSAARMLQEQFGVTAEVWSVTSFTELARDGIAAERRARLQPGA 418 +V+LL +G +L+E ++AA +L++ + V A+VWS SFT LARDG ER L P Sbjct: 731 NKLRVQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSFTLLARDGQDVERWNMLHPAE 790 Query: 419 KGALPYVTQQLTQQLQSGQAPVIAVSDYVRTLSESIRAYVPA--RYVTLGTDGFGRSDTR 476 Y Q L+ + P++ +DY+RT +E +RA+VP Y LGTDG+GRSDTR Sbjct: 791 APKKSY----FEQSLEGSEGPIVVSTDYMRTYAEQVRAFVPKGRSYKVLGTDGYGRSDTR 846 Query: 477 ANLRDFFEVDARWIAYTALAEVFGADAARLK-----DAAAKLGVDLDKP 520 A LR+FFEV+ ++ AL + AD ++K A AK G+D +KP Sbjct: 847 AKLREFFEVNRYFVTVAALKSL--ADEGKIKPEVVAQAIAKYGIDPNKP 893 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,623,848 Number of extensions: 69061 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 2 Length of query: 525 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 435 Effective length of database: 1,193,720 Effective search space: 519268200 Effective search space used: 519268200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)