BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0400.p (620 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 1134 0.0 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 242 8e-65 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 231 2e-61 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 187 2e-48 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 178 1e-45 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 1134 bits (2934), Expect = 0.0, Method: Compositional matrix adjust. Identities = 547/620 (88%), Positives = 578/620 (93%) Query: 1 MPVYRSRTTTQGRNMAGARALWRATGMKDGDFDKPIVAVVNSFTQFVPGHVHLKDLGQLV 60 MP YRS TTTQGRNMAGARALWRATGMKDGDF KPI+AVVNSFTQFVPGHVHLKDLGQLV Sbjct: 1 MPTYRSYTTTQGRNMAGARALWRATGMKDGDFTKPIIAVVNSFTQFVPGHVHLKDLGQLV 60 Query: 61 AREIEASGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCIS 120 AREIEA+GGVAKEFNTIAVDD G MLYSLPSRDLIADSVEYMVNAHCADAMVCIS Sbjct: 61 AREIEAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCIS 120 Query: 121 NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVVKVVNNDRKVIKLDLIDAMIKAGDSTV 180 NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKV+K VN D+KVIKLDL+DAMI+AGD + Sbjct: 121 NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGDKNI 180 Query: 181 SDADVAEIERSACPTCGSCSGMFTANSMNCLAEALGLALPGNGTIVATHADRKELFLGAG 240 SDADVAE+ERSACPTCGSCSGMFTANSMNCL E LGL+LPGNGTIVATHADRKELFL AG Sbjct: 181 SDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAG 240 Query: 241 RLIVELARRHYEQDDYSVLPRSIASKAAFENAMALDVSMGGSTNTVLHLLAAAHEAQVEF 300 RLIVELA+RHYEQDDYS+LPR+I +K +ENAM LDVSMGGSTNTVLHLLAAAHEA+VEF Sbjct: 241 RLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEF 300 Query: 301 TMADIDRISRKVPCLCKVAPMTDKYHIEDVHRAGGILGILGELARAGLLDTSLPTVHSKT 360 TMADIDRISR+VPCLCKVAPMT+KYHIEDVHRAGGI+ ILGELARAGLLDTS PTVHSKT Sbjct: 301 TMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKT 360 Query: 361 MGEAIEKYDIRVSSDAAVHKLFRAAPGGVPSQTAFSQAERYEHGDLDRSAGCIRDLAHAY 420 +GEAIEK+DIRVSSD AVHKLFRAAPGGVP+Q AFSQ ER+E DLDR+ GCIRD HAY Sbjct: 361 LGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAY 420 Query: 421 SKDGGLAVLYGNIAEKGCIVKTAGVDESILRFSGKARVFESQDAAVEGILGDSVHAGDVV 480 S+DGGLAVLYGNIAEKGCIVKTAGVDESIL+FSG ARVFESQDAAVEGILGD V AGDVV Sbjct: 421 SQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGDVV 480 Query: 481 IIRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG 540 I+RYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG Sbjct: 481 IVRYEGPKGGPGMQEMLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG 540 Query: 541 AIGLVEEGDMIDIDIPARTINLRVSAEVLAQRRAAMEALGHDAWQPLGRERVVSQALQAY 600 AIGLVEEGDMIDIDIP RTINLR+S++ LA+RR AMEA G AWQP R+RVVSQALQAY Sbjct: 541 AIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQPGNRQRVVSQALQAY 600 Query: 601 AALTTSADRGAVRDLSQLKR 620 AALTTSADRGAVRDLSQLKR Sbjct: 601 AALTTSADRGAVRDLSQLKR 620 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 242 bits (617), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 174/574 (30%), Positives = 296/574 (51%), Gaps = 66/574 (11%) Query: 11 QGRNMAGARALWRATGMKDGDFD-KPIVAVVNSFTQFVPGHVHLKDLGQLVAREIEASGG 69 Q R+ R+ + G+ FD +P++ + N++++ P + H + L + V I +GG Sbjct: 19 QDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRALAEQVKIGIWEAGG 78 Query: 70 VAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGM 129 EF +++ + + + MLY R+L + VE + A+ D +V + CDK TP + Sbjct: 79 FPLEFPVMSLGETL-LRPTAMLY----RNLASMDVEESIRANPLDGVVLLMGCDKTTPAL 133 Query: 130 LMAAMRLNIPVVFVSGGPMEAGKVV-KVVNNDRKVIKLDLIDAMIKAGDSTVSDADVAEI 188 LM A +++P + VSGGPM +GK + + + V ++ ++AG T + E Sbjct: 134 LMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMS---EDVRAGKMT--QEEFFEA 188 Query: 189 ERSACPTCGSCSGMFTANSMNCLAEALGLALPGNGTIVATHADRKELFLGAGRLIVELAR 248 E + G C M TA++M + EALG+ LP N I A A R L AGR IV++ Sbjct: 189 ESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMV- 247 Query: 249 RHYEQDDYSVLPRSIASKAAFENAMALDVSMGGSTNTVLHLLAAAHEAQVEFTMADIDRI 308 E+D ++ I ++ AFENA+ ++ ++GGSTN V+HLLA A ++ + D D I Sbjct: 248 ---EED---LVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDI 301 Query: 309 SRKVPCLCKVAPMTDKYHIEDVHRAGGILGILGELARAGLLDTSLPTVHSKTMGEAIEKY 368 +++PCL + P + K+ +ED + AGG+ ++ +L ++D + T + KT+ E + Sbjct: 302 GQQLPCLLDLQP-SGKFLMEDFYYAGGLPAVIRQL--ESVIDKTALTANGKTLWENCQD- 357 Query: 369 DIRVSSDAAVHKLFRAAPGGVPSQTAFSQAERYEHGDLDRSAGCIRDLAHAYSKDGGLAV 428 AP + + IR + + G+A+ Sbjct: 358 ----------------AP--------------------NWNEEVIRSFDKPFKEAAGIAI 381 Query: 429 LYGNIAEKGCIVKTAGVDESILRFSGKARVFESQDAAVEGILGDSVHAGD--VVIIRYEG 486 L GN+A G ++K + ++L+ G+A VFE+ D + I +++ + V++++ G Sbjct: 382 LKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCG 441 Query: 487 PKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIG 543 PKG PGM E M P ++ KG+ + +D R SG + G V+ H SPEAA GG + Sbjct: 442 PKGYPGMAEAGNMPLPPKILR-KGITDMVRV-SDARMSGTAYGTVVLHVSPEAAAGGPLA 499 Query: 544 LVEEGDMIDIDIPARTINLRVSAEVLAQRRAAME 577 +V+ GD I++D+ AR ++L VS E LA+RRA + Sbjct: 500 VVQNGDFIELDVEARKLHLDVSDEELARRRAQWQ 533 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 231 bits (588), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 179/576 (31%), Positives = 290/576 (50%), Gaps = 69/576 (11%) Query: 10 TQGRNMAGARALWRATGMKDGDFD-KPIVAVVNSFTQFVPGHVHLKDLGQLVAREIEASG 68 T +N R+ + G+ D +F KP++ + N++++ P + H + + + V R I +G Sbjct: 20 TADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKIAEHVRRGIIEAG 79 Query: 69 GVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPG 128 G EF + + + ML +R+L + VE + + DA+V ++ CDK TP Sbjct: 80 GFPVEFPVFSNGES-NLRPTAML----TRNLASMDVEESIRGNPIDAVVLLTGCDKTTPA 134 Query: 129 MLMAAMRLNIPVVFVSGGPMEAGKVV-KVVNNDRKVIKLDLIDAMIKAGDSTVSDADVAE 187 +LM A ++P + V+GGPM GK + + + V +L +KAG+ T+ D AE Sbjct: 135 LLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQL---SEQVKAGEITIHDFMAAE 191 Query: 188 --IERSACPTCGSCSGMFTANSMNCLAEALGLALPGNGTIVATHADRKELFLGAGRLIVE 245 + RSA G+C+ M TA++M C+AE+LG++LP N I A A R L +G IV+ Sbjct: 192 AGMSRSA----GTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVD 247 Query: 246 LARRHYEQDDYSVLPRSIASKAAFENAMALDVSMGGSTNTVLHLLAAAHEAQVEFTMADI 305 + +E S I ++ AFENA+ + ++GGSTN V+HL A A V+ + D Sbjct: 248 MV---WEGLTLS----KILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDW 300 Query: 306 DRISRKVPCLCKVAPMTDKYHIEDVHRAGGILGILGELARAGLLD-TSLPTVHSKTMGEA 364 RI R P + + P + +Y +E+ + AGG+ +L L A LL TV+ +TM + Sbjct: 301 TRIGRGTPTIVDLQP-SGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDN 359 Query: 365 IEKYDIRVSSDAAVHKLFRAAPGGVPSQTAFSQAERYEHGDLDRSAGCIRDLAHAYSKDG 424 ++ D + +D V R LA +DG Sbjct: 360 VK--DAPIYNDEVV-----------------------------------RPLAKPLIEDG 382 Query: 425 GLAVLYGNIAEKGCIVKTAGVDESILRFSGKARVFESQDAAVEGILGD--SVHAGDVVII 482 G+ +L GN+A +G ++K + +++ G+A VFE + E I V A V+++ Sbjct: 383 GICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASCVLVM 442 Query: 483 RYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEG 539 + GPKG PGM E M P + + G+ K +D R SG + G VI H +PEAA G Sbjct: 443 KNVGPKGYPGMAEVGNMGLPPKVLAT-GV-KDMVRISDARMSGTAYGTVILHVAPEAAAG 500 Query: 540 GAIGLVEEGDMIDIDIPARTINLRVSAEVLAQRRAA 575 G +G+V++GD I++D A + L +S E + +R A Sbjct: 501 GPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEA 536 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 187 bits (475), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 167/560 (29%), Positives = 267/560 (47%), Gaps = 79/560 (14%) Query: 34 KPIVAVVNSFTQFVP-GHVHLKDLGQLVAREIEASGGVAKEFNTIAVDDGIAMGHGGMLY 92 +PI+ + S + P +HL +L + V I +GG+ EF + + + Sbjct: 47 RPIIGIAQSGSDISPCNRIHL-ELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAI-- 103 Query: 93 SLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGK 152 R+L + +++ + DA+V + CDK TP +MAA ++IP + +SGGPM G Sbjct: 104 ---DRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGW 160 Query: 153 VV-KVVNNDRKVIKLDLIDAMIKAGDSTVSDADVAEIERSACPTCGSCSGMFTANSMNCL 211 + ++V + + K ++ AG ++ + EI ++ P+ G C+ M TA++MN + Sbjct: 161 MDGELVGSGSAIWKGR---KLLSAG--SIDNEKFLEIAAASAPSSGHCNTMGTASTMNAM 215 Query: 212 AEALGLALPGNGTIVATHADRKELFLGAGRLIVELARRHYEQDDYSVLPRSIASKAAFEN 271 AEALG++L G I A + +R ++ GR IV +A YE + P +I ++ AF + Sbjct: 216 AEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA---YED----LRPSAILTRDAFLD 268 Query: 272 AMALDVSMGGSTNTVLHLLAAAHEAQVEFTMADIDRISRKVPCLCKVAPMTDKYHIEDVH 331 A+ ++ ++GGSTN H++A A A VE D + VP L + P KY E H Sbjct: 269 AIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPLLLNMQP-AGKYLGERFH 327 Query: 332 RAGGILGILGELARAGLLDTSLPTVHSKTMGEAIEKYDIRVSSDAAVHKLFRAAPGGVPS 391 RAGG+ I+ EL +AG L T KTM E ++ R S+D + F AAP Sbjct: 328 RAGGVPAIMWELQQAGKLRAERITATGKTMAENLQG---RASNDREMIYPF-AAP----- 378 Query: 392 QTAFSQAERYEHGDLDRSAGCIRDLAHAYSKDGGLAVLYGNIAEKGCIVKTAGVDESI-- 449 +R+ A G VL GN+ + I+KT+ + E+ Sbjct: 379 ---------------------LRERA-------GFLVLKGNLFDF-AIMKTSVISETFRE 409 Query: 450 --LRFSGKARVFESQDAAVEGILGDSVHA-----------GDVVIIRYEGPKGGPGMQEM 496 L G+ +FE + +G D HA ++ IR GP G PG E+ Sbjct: 410 RYLSTPGQENIFECRAVVFDG--SDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEV 467 Query: 497 --LYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDID 554 + P + +G+ L DGR SG S I +ASPE+A GG + + +GD + ID Sbjct: 468 VNMQPPDALIKRGVSTLPTL-GDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRID 526 Query: 555 IPARTINLRVSAEVLAQRRA 574 + N+ VS E LA+R++ Sbjct: 527 LNTGECNMLVSEEELARRKS 546 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 178 bits (452), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 158/543 (29%), Positives = 254/543 (46%), Gaps = 41/543 (7%) Query: 35 PIVAVVNSFTQFVPGHVHLKDLGQLVAREIEASGGVAK-EFNTIAVDDGIAMGHGGMLYS 93 P +A+V+++ + H + Q++ +G VA+ + A+ DG+ G GM S Sbjct: 68 PSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQAGMELS 127 Query: 94 LPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRL-NIPVVFVSGGPMEAGK 152 L SRD IA + ++ + DA + + CDKI PG+L+ A+ ++P VFV GPM +G Sbjct: 128 LFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSG- 186 Query: 153 VVKVVNNDRKVIKLDLIDAMIKAGDSTVSDADVAEIERSACPTCGSCSGMFTANSMNCLA 212 + N+D+ I+ + AG + A++ E E + G+C+ TANS L Sbjct: 187 ---MSNSDKVKIR-----QLYTAGK--IGRAELLEAESKSYHGAGTCTFYGTANSNQMLM 236 Query: 213 EALGLALPGNGTIVATHADRKELFLGAGRLIVELARRHYEQDDYSVLPRSIASKAAFENA 272 EA+GL LPG I R L A + ++ + Y+ + R I K+ Sbjct: 237 EAMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQ---YTPVGRMIDEKSIVNAI 293 Query: 273 MALDVSMGGSTNTVLHLLAAAHEAQVEFTMADIDRISRKVPCLCKVAPMTDKYHIEDVHR 332 +AL + GGSTN LHL+A A A + D D++S VP + K+ P + H Sbjct: 294 VALHAT-GGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYP-NGTADVNHFHA 351 Query: 333 AGGILGILGELARAGLLDTSLPTVHSKTMGEAIEKYDIRVSSDAAVHKLFRAAPGGVPSQ 392 AGG +L EL AGL+ + T+ + + + ++ + + PG V Sbjct: 352 AGGTGFVLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGE-------NGRPGIVTWH 404 Query: 393 TAFSQAERYEHGDLDRSAGCIRDLAHAYSKDGGLAVLYGNIAEKGCIVKTAGVDESILRF 452 A +Q+ GD G + +A ++ DGGL +L GN+ ++K + V Sbjct: 405 DAPAQS-----GD----EGVLGTVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVV 453 Query: 453 SGKARVFESQDAAVEGILGDSVHAGDVVIIRYEGPK--GGPGMQEMLYPTSYIKSKGLGK 510 A VF SQ+A + + V +I ++GP+ G P + + ++ K G+ Sbjct: 454 EAPAVVFHSQEAFMAAFKAGQLDRDFVAVITFQGPRANGMPELHALTPALGILQDK--GR 511 Query: 511 ACALFTDGRFSGGSSGLVIG-HASPEAAEGGAIGLVEEGDMIDIDIPARTINLRVSAEVL 569 AL TDGR SG S + H +PE GG +G V +GD+I +D A T+ +V A Sbjct: 512 HVALVTDGRMSGASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEW 571 Query: 570 AQR 572 A R Sbjct: 572 AAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,887,781 Number of extensions: 79537 Number of successful extensions: 325 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 305 Number of HSP's successfully gapped: 8 Length of query: 620 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 529 Effective length of database: 1,188,985 Effective search space: 628973065 Effective search space used: 628973065 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)