BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0387.p (490 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase... 570 e-163 gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase... 184 1e-47 >gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 525 Score = 570 bits (1468), Expect = e-163, Method: Compositional matrix adjust. Identities = 303/523 (57%), Positives = 372/523 (71%), Gaps = 36/523 (6%) Query: 4 NWTDGYVTDIGYTHGYYPQLNPARLQLAFAAAGLDSPLVRTACELGFGQGLSINIHAAAS 63 NW DGYV DI YT+GYYP +NPARL LAFA GL +P V+TACELGFGQGLS NIHAAAS Sbjct: 3 NWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAVQTACELGFGQGLSSNIHAAAS 62 Query: 64 PVTWYGNDFNAAQAAHAQTLAQAACSGAQLEQASFAEFCTREELPQFDFIAMHGIWSWVS 123 W+GNDFN QAA AQ LA A+ SGAQLE +SF+ FC R++LP FDFIA+HGIWSWVS Sbjct: 63 TTRWWGNDFNPRQAAFAQELAAASGSGAQLEDSSFSRFCRRDDLPDFDFIALHGIWSWVS 122 Query: 124 ADNRALIRDFVDRRLKRGGVLYASYNTQPGWAAFMPLRDLLLRHFDMPSNAGKG--SAER 181 +NR +I DF+DR+LK GGVLY SYNTQPGW+AFMPLR+LL++H N +G +A R Sbjct: 123 QENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQHTQQSGNEEQGLPTAAR 182 Query: 182 IDAALDFAERMFAAEPVYARANPFMQERIAILRKQSRHYLAHEYFNRDWHVESFADMAAI 241 IDAAL FA R+F A+PVYA+ANPFM++R+ +L++QSR YLAHEYFNRDWHVESFADM Sbjct: 183 IDAALAFAARLFEADPVYAQANPFMRDRLKVLQQQSRSYLAHEYFNRDWHVESFADMHRR 242 Query: 242 WSGAGLEFACSADFRDHLDMANLTPAQREFCDAVDDRHLRQSVRDFMVNQQFRRDYWVRG 301 +SGAGLEFAC A + DHLDMANLTPAQR+ + D L+QSVRDFMVNQQFRRDYWVRG Sbjct: 243 FSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFMVNQQFRRDYWVRG 302 Query: 302 AQALDAASHQALLAQQRVTLLTVPGQIPMTLKTVSSEITLNQHIYGPVIAVLADGLPHSL 361 AQALD LA Q V LLT P ++P+T++ V+SEITLN+ IY P++ L+D PH+L Sbjct: 303 AQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEPILQALSDYQPHTL 362 Query: 362 GEIAGIVGSRNLGLQQVLDTLMMLIGNGHXKAVHGNAD---------------------- 399 ++A + RNL L QVLD+++MLIG GH + D Sbjct: 363 VDLAASLAHRNLNLAQVLDSVLMLIGTGHAAPLPAEPDAATRAQIERRTGDLNAWLLQRA 422 Query: 400 ------------GDIEHLCSPVTGGGVPADRIQQLFMLAVLEGQETPDAIVGFVWRHISA 447 G+I HL SPV GGGV DR++QLF+LA + DA VWR+I+A Sbjct: 423 ALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFLLARQSCGDDVDAWPAQVWRYIAA 482 Query: 448 GGKRIVRDGIRLEDEQANIDELSLQAQRFFVERLPLLQALQVI 490 GKR++R+G+RLE E N+ EL+ QA+ F RLPLL+AL V+ Sbjct: 483 QGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKALGVV 525 >gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 523 Score = 184 bits (468), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 149/509 (29%), Positives = 233/509 (45%), Gaps = 31/509 (6%) Query: 4 NWTDGYVTDIGYTHGYYPQLNPARLQLAFAAAGLDS-PLVR--TACELGFGQGLSINIHA 60 W+ GYV I Y G++P+ +P L A A G++ PL R T ELG GQGL+ + A Sbjct: 2 QWSSGYVHGIDYPVGFFPEQSPVHLSFACLAQGVEPVPLDREFTYMELGCGQGLTSTVLA 61 Query: 61 AASPV-TWYGNDFNAAQAAHAQTLAQAA-CSGAQLEQASFAEFCTRE-ELPQFDFIAMHG 117 AA+P +Y DF Q AQ +A AA + + SF + E +LP FDF+ M+G Sbjct: 62 AANPQGRFYAVDFLPTQVRTAQDMASAAGIDNLTVLECSFEQMARGEIDLPAFDFVTMYG 121 Query: 118 IWSWVSADNRALIRDFVDRRLKRGGVLYASYNTQPGWAAFMPLRDLLLRHFDMPSNAGKG 177 ++SWV NR I DF+ R LK GG++Y +YN PGW++ + L+ +LR F + Sbjct: 122 VYSWVDRTNRGHILDFLRRYLKPGGIVYLNYNALPGWSSALSLQR-MLREFGRHDMCDRQ 180 Query: 178 SAERIDAALDFAERMFAAEPVYARANPFMQERIAILRKQSRHYLAHEYFNRDWHVESFAD 237 + AL A A A ++ R+ L YLAHEY N+ W D Sbjct: 181 AQLHQGGALVRALSQAGAAAFGAAEATGLRRRLRALESDEPGYLAHEYLNQGWDALFSID 240 Query: 238 MAAIWSGAGLEFACSADFRDHLDMANLTPAQREFCDAVDDRHLRQSVRDFMVNQQFRRDY 297 + + A L++ SA+ L+ AQ+ + +R+ V D + N FR D Sbjct: 241 VIQDLAQAKLDYVGSAELAYGFPELYLSHAQQSLLAQQPNAGMRELVWDQICNSSFREDI 300 Query: 298 WVRGAQALDAASHQALLAQQRVTLLTVPGQIPMTLKTVSSEITLNQHIYGPVIAVLADGL 357 +VRGA+ + A L + + L P +I + L + + H Y PV+ L Sbjct: 301 YVRGARRMSPHQRDAWLERCGLALTEAPSRITLELPLAIGNVRADAHTYQPVMDALCQR- 359 Query: 358 PHSLGEIAGIVGSRN--LGLQQVLDTLMMLIGNGHX-------------------KAVHG 396 PHS E+A + R GL+++ ++L + + + G Sbjct: 360 PHSFAELAALPALRKQGRGLREIASMAILLSASRQAAPFFLSAAAQPRQAAQRLNRLIAG 419 Query: 397 -NADGD-IEHLCSPVTGGGVPADRIQQLFMLAVLEGQETPDAIVGFVWRHISAGGKRIVR 454 +A+ D + L SP+ G G+P+ +Q++ + + Q P+ V + + I G + R Sbjct: 420 WSAESDCFQALASPLLGNGLPSGLLQRVAFDQLSQAQSQPEQWVLPMAQAIHRQGTHLQR 479 Query: 455 DGIRLEDEQANIDELSLQAQRFFVERLPL 483 G L + EL AQ +RLP+ Sbjct: 480 GGAPLSTREEIHAELLALAQAICTQRLPV 508 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,642,306 Number of extensions: 73173 Number of successful extensions: 235 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 2 Length of query: 490 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 401 Effective length of database: 1,198,455 Effective search space: 480580455 Effective search space used: 480580455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)