BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0359.p (232 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 90 2e-19 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 89 4e-19 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 75 4e-15 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 66 2e-12 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 65 5e-12 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 59/179 (32%), Positives = 94/179 (52%), Gaps = 14/179 (7%) Query: 21 IGASAGGLDALLRLFENMPAANGMAFVIVLHLSPKHESNIALLLQKSTKMPVRQVNEPIT 80 IGASAGG++AL + + +A ++VLHL + S +A + + PV + + Sbjct: 19 IGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDKEP 78 Query: 81 IQNNNVYVIPPSKSLLM---RDGQLVV---EPMV---RPRGRAVAIDMFFRTLADTHTVR 131 Q ++ PP LL+ DG ++ + +V RP AID+ F + AD + R Sbjct: 79 AQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRP-----AIDVLFESAADVYGPR 133 Query: 132 SVGIILSGTGSDGAVGIARIKEAGGITIAQDPKDAEYDGMPQNAIETRAIDFVLPVTEI 190 +GI+L+G DGA G+ I AGG+T+ Q P+ A+ MP A+ ID+VL + +I Sbjct: 134 LLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQI 192 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 88.6 bits (218), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 59/187 (31%), Positives = 97/187 (51%), Gaps = 11/187 (5%) Query: 17 PIVGIGASAGGLDALLRLFENMPAANGMAFVIVLHLSPKHESNIALLLQKSTKMPVRQVN 76 P+V +GASAGG AL L + +P A VIV H+ +A LQ+ + + V Sbjct: 159 PLVAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQ 218 Query: 77 EPITIQNNNVYVIPPSKSLLMRDGQLV--VE----PMVRPRGRAVAIDMFFRTLADTHTV 130 E Q V + ++ L++++G + VE + RP ++D+FF+++ Sbjct: 219 EGQAPQRGAVLLAASNRHLVVKEGGRLGYVEGAPNDVYRP-----SVDVFFQSIVRHWPG 273 Query: 131 RSVGIILSGTGSDGAVGIARIKEAGGITIAQDPKDAEYDGMPQNAIETRAIDFVLPVTEI 190 + G++L+G G DGA G+ ++++G T+AQD K GMP+ A+ A VLPVT + Sbjct: 274 KVAGLLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGM 333 Query: 191 PQRLMEV 197 RL + Sbjct: 334 AARLQAI 340 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 75.1 bits (183), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 55/177 (31%), Positives = 88/177 (49%), Gaps = 5/177 (2%) Query: 18 IVGIGASAGGLDALLRLFENMPAANGMAFVIVLHLSPKHESNIALLLQKSTKMPVRQVNE 77 IV IGAS GG AL R+ + + V+V H+ + + A L + + V++ Sbjct: 196 IVAIGASTGGPQALERVLGKL-SQRCPGIVVVQHMPQRFTRSFAERLHRISGAEVKEAEH 254 Query: 78 PITIQNNNVYVIPPSKSLLMR-DG-QLVVEPMVRP--RGRAVAIDMFFRTLADTHTVRSV 133 I V + P + L++R +G Q VE + P ++D+ FR+LA +V Sbjct: 255 LDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSNAV 314 Query: 134 GIILSGTGSDGAVGIARIKEAGGITIAQDPKDAEYDGMPQNAIETRAIDFVLPVTEI 190 GII++G G DGA G+ + + GG T AQD + GMP+ AI+ + V+ + I Sbjct: 315 GIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLEHI 371 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 66.2 bits (160), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 60/201 (29%), Positives = 92/201 (45%), Gaps = 16/201 (7%) Query: 4 LEQTLTPPSSLQFPIVGIGASAGGLDALLRLFENMPAANGMAFVIVLHLSPKHESNIALL 63 L Q P +S + I+ IGAS GG +A+ MP+ + +I H+ + A Sbjct: 153 LPQIRNPLTSSEKLII-IGASTGGTEAIKEFLIRMPS-DCPGILITQHMPEGFTRSFAQR 210 Query: 64 LQKSTKMPVRQVNEPITIQNNNVYVIPPSKSL-LMRDG-----QLVVEPMV---RPRGRA 114 L K+ V + + + Y+ P L L+R G QL P V RP Sbjct: 211 LNNLCKISVVESAGNERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRP---- 266 Query: 115 VAIDMFFRTLADTHTVRSVGIILSGTGSDGAVGIARIKEAGGITIAQDPKDAEYDGMPQN 174 ++D+ F++ A +VG+IL+G G DGA G+ ++ AG AQD GMP+ Sbjct: 267 -SVDVLFQSAATCAGKNAVGVILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPRE 325 Query: 175 AIETRAIDFVLPVTEIPQRLM 195 AI A VL + +P R++ Sbjct: 326 AIAVGAAHEVLALQAMPGRVL 346 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 65.1 bits (157), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 49/184 (26%), Positives = 84/184 (45%), Gaps = 9/184 (4%) Query: 18 IVGIGASAGGLDALLRLFENMPAANGMAFVIVLHLSPKHESNIALLLQKSTKMPVRQVNE 77 +V IG S GG L + +PA+ ++ H+ + A + K + V +V Sbjct: 166 LVLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAA 225 Query: 78 PITIQNNNVYVIPPSKSLLM--RDGQLVVEPMVRPRG----RAVAIDMFFRTLADTHTVR 131 P +Q +VY+ +L+ R L V P +P ++++ R+ + R Sbjct: 226 PTVVQGGHVYIARGGADMLVAKRGSHLSVLP--KPESPTHLWHPSVELLARSALQSMAAR 283 Query: 132 S-VGIILSGTGSDGAVGIARIKEAGGITIAQDPKDAEYDGMPQNAIETRAIDFVLPVTEI 190 +G++L+G G+DGA A I GG TIA+ GMP++ I+ VLP ++I Sbjct: 284 QLIGVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKI 343 Query: 191 PQRL 194 +L Sbjct: 344 ATQL 347 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 692,130 Number of extensions: 28843 Number of successful extensions: 85 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 80 Number of HSP's successfully gapped: 5 Length of query: 232 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 150 Effective length of database: 1,231,600 Effective search space: 184740000 Effective search space used: 184740000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)