BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0002.0355.p
         (336 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309488|ref|YP_003773580.1| 4-hydroxybutyrate dehydrogenase...   110   1e-25
gi|300313015|ref|YP_003777107.1| maleylacetate reductase [Herbas...    76   3e-15

>gi|300309488|ref|YP_003773580.1| 4-hydroxybutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 379

 Score =  110 bits (275), Expect = 1e-25,   Method: Compositional matrix adjust.
 Identities = 95/324 (29%), Positives = 159/324 (49%), Gaps = 23/324 (7%)

Query: 8   RALGLIQEIEQLLGDQLAYVIEDVSP-NPDVALLAGMYDRFWQNDEACDTVVALGGGSAI 66
           R  GL+ ++   L + +  V+ D +P NP+   +      F Q+   CD +VA+GGGS+I
Sbjct: 41  RNAGLLDKVLGQLKEGVGAVVYDQTPPNPNEGAVRAAVALFRQH--GCDGIVAVGGGSSI 98

Query: 67  DTAKALIV-GTQSGRFEELLALLADGKPFTPYRVKTLIAIPTTAGTGSEVTPWATIW--D 123
           D AK + V GT  G  +   AL+  G      +   +IAIPTTAGTGSEV   A +   D
Sbjct: 99  DLAKGVAVCGTHEGPLKSF-ALIEGGLANITAKTAPVIAIPTTAGTGSEVGRGAILILDD 157

Query: 124 AEKQKKYSLHLDCTWPRAAIVDPDLMLSLPRHVTISTGLDALSHALESIWNVNANPVSDA 183
             K    S +L    P+ AI DP+L L LP  +T +TG+DA++H LE+    + NP +D 
Sbjct: 158 GRKVGIISPYL---VPKLAICDPELTLGLPPLMTAATGMDAIAHCLETFMAPSFNPPADG 214

Query: 184 FAISAINDIMTCLPLLAKDLNNKALRSSMALAAMKAGLAFSNTKTALAHSISYEM-TLRH 242
            A+  +      +    ++  ++  R +M  A+M+  +AF        HS+S+ +  +  
Sbjct: 215 IALDGLWRAWAHIERATREPGDREARLNMMSASMQGAMAFQK-GLGCVHSLSHSLGGINP 273

Query: 243 GLPHGIACSFTLPLVLKMAWGETPE---RDAVLQKV-----FNSDCQTAVARLRNFLHQL 294
            L HG   +  LP ++  A+ E+     +D  + ++       S  +   A +R    +L
Sbjct: 274 RLHHGTLNAIFLPAII--AFNESAASMVKDNKMARMAHAMGLGSGAEIGPA-IREMSRRL 330

Query: 295 DVKTEFSDYGVSSPEALEMVQGAM 318
            +     + GV+     +++QGA+
Sbjct: 331 GLPAGLGELGVTESMFPQIIQGAL 354


>gi|300313015|ref|YP_003777107.1| maleylacetate reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 356

 Score = 76.3 bits (186), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 55/202 (27%), Positives = 92/202 (45%), Gaps = 27/202 (13%)

Query: 55  DTVVALGGGSAIDTAKALIVGTQSGRFEELLALLADGKPFTPYRVKTLIAIPTTAGTGSE 114
           D  +A+GGGS +   KA+ + ++                        ++AIPTT   GSE
Sbjct: 87  DCAIAIGGGSTMGLGKAIALESE----------------------LPILAIPTTY-AGSE 123

Query: 115 VTPWATIWDAEKQKKYSLHLDC-TWPRAAIVDPDLMLSLPRHVTISTGLDALSHALESIW 173
           +TP   + +A ++K      D    PR  I DP L L LP  +++++G++A++HA E ++
Sbjct: 124 MTPIYGLTEAGRKKTGR---DARVLPRTVIYDPALSLGLPLDLSVTSGINAMAHAAEGLY 180

Query: 174 NVNANPVSDAFAISAINDIMTCLPLLAKDLNNKALRSSMALAAMKAGLAFSNTKTALAHS 233
             +ANP+    A   +  +   LP LA   ++   RS     A   G    N   +L H 
Sbjct: 181 APDANPIMSLLAEEGLRALAEGLPQLAARADDIDARSRCLYGAWLCGTVLGNVGMSLHHK 240

Query: 234 ISYEMTLRHGLPHGIACSFTLP 255
           + + +   + LPH    +  LP
Sbjct: 241 LCHTLGGSYNLPHAQTHTVILP 262


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.133    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,069,516
Number of extensions: 44349
Number of successful extensions: 129
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 126
Number of HSP's successfully gapped: 2
Length of query: 336
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 250
Effective length of database: 1,212,660
Effective search space: 303165000
Effective search space used: 303165000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)