BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0354.p (432 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 75 9e-15 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 67 3e-12 gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 60 4e-10 gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 56 7e-09 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 55 1e-08 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 75.1 bits (183), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 97/404 (24%), Positives = 167/404 (41%), Gaps = 28/404 (6%) Query: 15 TTSFRRAQWRMLFAAMLCYLFFYTGRQTFGFAIPGIQKEFGLSKEMLGWVSSAMLWSYAI 74 + +R Q L ML + Y R T A + +E LS +G + SA +YA Sbjct: 8 SPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAF 67 Query: 75 GQAINGNLADKYGGRRIMSLGAVLSCFANWVVSFSSGFVTLVFPWAVNGYFQALGWAP-- 132 Q G + DK+G R ++ G F V + GFV + + V F LG AP Sbjct: 68 SQLPIGAMLDKFGARVMLGAG----MFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQF 123 Query: 133 --GSRLLANWWGIGERGKVYGFYVFAAGCASVLSYVTSIAVLDVFHLDWRWIFRIPVLLM 190 G+++++ W+ + ERG G +V ++ L+ I + + WR +F I +L Sbjct: 124 PAGAKVVSEWFAMRERGGPTGIFVASSTIGPALA--PPILTILMLSFGWRMMFVIMGVLG 181 Query: 191 LAGGIFFYLVARERPEDMGFKPLDTGVADAVDKAHAVQDDQTESSWSRYKAVLKNWRLDI 250 +A + +YL R R E + + V D + + SWS +K+ L +R Sbjct: 182 IAVSVGWYLFYRNRNE---VQLTPSEVLYLADDHDNDPNANAKLSWSEWKS-LFTYRTTW 237 Query: 251 AALSLGFQNAAR--YGLIVWVPVHFFGSNWAKSPDAFINPKWISIALPVGMAIGALSNGW 308 L GF + + W+P + + + W+ + + +G LS+G+ Sbjct: 238 GML-FGFMGVIYMVWLYLTWLPAYL----EHERHLSIARTGWVVVIPYIFGTLGMLSSGY 292 Query: 309 ISDKLFGSKRYKAI--MLYMVLGAITTMWMFTIPG----NSLIGVVALFLCGFFVYGPAS 362 I+D L + AI + + + FTIP ++ + +V + L FV + Sbjct: 293 IADGLM-KRGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASG 351 Query: 363 SFWALCPDLVGSRRAGTATGIMNFSSYLFAGLGEPLIGRMLDTS 406 WAL + R + + NF YL L + G ++D + Sbjct: 352 GAWALVSVAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQT 395 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 67.0 bits (162), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 79/351 (22%), Positives = 143/351 (40%), Gaps = 40/351 (11%) Query: 19 RRAQWRMLFAAML--CYLFFYTGRQTFGFAIPGIQKEFGLSKEMLGWVSSAMLWSYAIGQ 76 ++ +R + A++ Y R G A+P ++KEF +S G ++S L++Y++ Q Sbjct: 11 KKTHFRWVVGALIFIVYTVAAADRANLGVALPFLRKEFVMSNTEAGALASIFLFAYSLAQ 70 Query: 77 AINGNLADKYGGRRIMSLGAV-LSCFANWVVSFSSGFVTLVFPWAVNGYFQALGWAPG-- 133 +G ++G R+ SL + S F + + SS +F + LG A G Sbjct: 71 LPSGLAYTRFGIPRVFSLAMLATSVFTGLIGTASSLLALKIFRF-------GLGLAEGPL 123 Query: 134 ----SRLLANWWGIGERGKVYGFYVFAAGCASVLSYVTSIAVLDVFHLDWRWIFRIPVLL 189 + + NW+ E+G + G ++ AA VL V + + +++ WR IF + Sbjct: 124 PIGITSTINNWFPAREKGTMTGIFLAAAKFGPVL--VPPVCAVIMYYWGWRHIFYFFAVP 181 Query: 190 MLAGGIFFYLVARERP--------EDMGFKPLDTGVADAVDKAHAVQDD----------Q 231 + + + +V + RP E++ G A D AV D Sbjct: 182 GVILSVVWLMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLDRIIRRS 241 Query: 232 TESSWSRYKAVLKNWRLDIAALSLGFQNAARYGLIVWVPVHFFGSNWAKSPDAFINPKWI 291 ++ K V +W + AAL F A L+ W+P + + N + Sbjct: 242 KVATIETTKGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGY----SIANMGMV 297 Query: 292 SIALPVGMAIGALSNGWISDKLFGSKRYKAIMLYMVLGAITTMWMFTIPGN 342 + A VG IG L G ++D+ +R A+M+ I + + P + Sbjct: 298 AAAPWVGAVIGNLLGGAMTDRWLEKRRKPAMMMSAFFTTIMMVILINSPAD 348 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 59.7 bits (143), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 109/450 (24%), Positives = 179/450 (39%), Gaps = 71/450 (15%) Query: 17 SFRRAQWRMLFAAMLCYLFFYTGRQTFGFAIPGIQKEFGLSKEMLGWVSSAMLWSYAIGQ 76 + R+ WR+L MLCYLF + R G A + + GLS +M G+ SS +Y I + Sbjct: 14 TVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYFIFE 73 Query: 77 AINGNLADKYGGRR-----IMSLGAVLSCFANWVVSFSSGFVTLVFPWAVNGYFQALGWA 131 + K+G R+ +++ G + +C A S FV + A G+F Sbjct: 74 VPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILGAAEAGFF------ 127 Query: 132 PGSRLLANWW-GIGERGKVYGFYVFAAGCASVLSYVTSIAVLDVFHL----DWRWIFRIP 186 PG L +W R ++ ++ A AS ++ S +L + + W W+F + Sbjct: 128 PGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFMLE 187 Query: 187 VLLMLAGGIFFYLVARERPEDMGFKPLDTGVADAVDKAHAVQDDQ---------TESSWS 237 L + G+ +RP D+A + D+Q E++ Sbjct: 188 GLPTVVLGLVCLSFLTDRP----------------DQARWLSDEQRQWLTETMNKEAASR 231 Query: 238 RYKAVLKNWRLDIA------ALSLGFQNAARYGLIVWVPVHFFGSNWAKSPDAFIN--PK 289 K + W+L AL +AA L VW P + +N P Sbjct: 232 AAKQHIPLWKLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPY 291 Query: 290 WISIALPVGMAIGALSNGWISDKLFGSKRYKAIMLYMVLGAITTMWMFTIPGNSLIGVVA 349 I+ L V G SD+ + + AI L ++ G + + NSL VV Sbjct: 292 LIASVLMVWW-------GRHSDRTGERRWHTAIPLMLIGGGLALASL----ANSLAPVVL 340 Query: 350 LF---LCGFFVY-GPASSFWALCPD--LVGSRRAGTATGIMNFSSYLFAGLGEPLIGRML 403 L L G + + GP FWA+ GS AG A GI S+ + GL + G + Sbjct: 341 LLTMVLIGAYSFKGP---FWAMASSWLATGSAAAGLA-GINAVSNLIGGGLMVNVYGWVK 396 Query: 404 DTSGDTSL-VFPIVAVSCAISAVVAFFIKR 432 D +G ++ + P+V +S ++ F ++ Sbjct: 397 DATGSYAMALLPMVGLSVVSVGILLFLSRK 426 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 55.8 bits (133), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 88/356 (24%), Positives = 139/356 (39%), Gaps = 51/356 (14%) Query: 43 FGFAIPGIQKEFGLSKEMLGWVSSAMLWSYAIGQAINGNLADKYGGRRIMSLGAVLSCFA 102 + F IP + + +SK G++++ L + AIG G LAD+YG R++ + F Sbjct: 31 YTFLIPTLLAAWSMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWTVLWFAFF 90 Query: 103 NWVVSFSSGFVTLVFPWAVNGYFQALGWAPGSRLLANWWGIGERGKVYGFYV--FAAGCA 160 ++ F++ F L F A+ G+ WA GS L+A RGK G +A G A Sbjct: 91 TFLSGFTTSFGQLFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQSSWAVGWA 150 Query: 161 -SVLSYVTSIAVLDVFHLDWR---WIFRIPVLLMLAGGIFFYLVARERPEDMGFKPLDTG 216 S L++ AVL W+ WI +P LL L + R E + Sbjct: 151 GSALAFWGVYAVLPP-ETAWKVLFWIGVLPALLTL-------YIRRAISEPAVY------ 196 Query: 217 VADAVDKAHAVQDDQTESSWSRYKAVLKNWRLDIAALSLGFQNAARYGLIVWVPVHFFGS 276 +A + Q + + L L + L+ G Q A Y + W+P Sbjct: 197 ---LATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQG-AYYSVTTWLPTFLK-- 250 Query: 277 NWAKSPDAFINPKWISIALPVGMAIGALSNGWISDKLFGSKRYKAIMLYMVLG------- 329 + + + + L G G L++ W+SD + R + ML+ +G Sbjct: 251 --TERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTI---GRRRCFMLFAAMGIVLVLSY 305 Query: 330 ---AITTMWMFTIPGNSLIGVVALFLCGFFVYGPASSFWALCPDLVGSRRAGTATG 382 IT WM + F+ GFF+ G S A +L S G+ G Sbjct: 306 TQMIITDAWMLALG----------FVLGFFLSGIFSGMGAYLSELFPSDVRGSGQG 351 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 55.1 bits (131), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 97/428 (22%), Positives = 178/428 (41%), Gaps = 32/428 (7%) Query: 18 FRRAQWRMLFAAMLCYLFFYTGRQTFGFAIPGIQKEFGLSKEMLGWVSSAMLWSYAIGQA 77 +++ WR++ MLCY+ Y R GFA + ++ G S+ + G + Y + + Sbjct: 22 YKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEV 81 Query: 78 INGNLADKYGGR----RIMSLGAVLSCFANWVVSFSSGFVTLVFPWAVNGYFQALGWAPG 133 + L K G R RIM +LS A V ++ F L F + G +A G+ PG Sbjct: 82 PSNILMHKVGARIWIARIMITWGILSA-AFMFVQNATQFYILRF---LLGLAEA-GFYPG 136 Query: 134 SRL-LANWWGIGERGKVYGFYVFAAGCASVLSYVTSIAVLDVFHLD-----WRWIFRIPV 187 L L W+ R KV ++ A +L S ++D FH + W+W+F I Sbjct: 137 IILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEA 196 Query: 188 L--LMLAGGIFFYLVARERPEDMGFKPLDTGVADAVDKAHAVQDDQTESSWSRYKAVLKN 245 + +++ YL D+ A +A D + + S + A++K+ Sbjct: 197 IPAVLIGVATVLYL-----DNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKD 251 Query: 246 WRLDIAALSLGFQNAARYGLIVWVPVHFFGSNWAKSPDAFINPKWISIALPVGMAIGALS 305 R+ + L +YGL +W+P + + + + A+P G AI A++ Sbjct: 252 ARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLS-----AIPFGCAIIAMN 306 Query: 306 -NGWISDKLFGSKRYKAIMLYMVLGAITTMWMFTIPGNSLIGVVALFLCGFFVYGPASSF 364 G +D++ +R +++ ++G + + N+ + + +L L V A F Sbjct: 307 LIGRSADRM--RERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLF 364 Query: 365 WALCPDLVGSRRAGTATGIMNFSSYLFAGLGEP-LIGRMLDTSGDTSLVFPIVAVSCAIS 423 W+L + A +N L AG P LIG + D + + + ++AV + Sbjct: 365 WSLPTAFLSGAAAAVGIAAINSVGNL-AGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVG 423 Query: 424 AVVAFFIK 431 ++ K Sbjct: 424 SIATLATK 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.460 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,428,500 Number of extensions: 63444 Number of successful extensions: 354 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 345 Number of HSP's successfully gapped: 14 Length of query: 432 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 344 Effective length of database: 1,203,190 Effective search space: 413897360 Effective search space used: 413897360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)