BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0352.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 258 8e-70 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 240 1e-64 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 219 5e-58 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 204 1e-53 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 201 2e-52 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 258 bits (659), Expect = 8e-70, Method: Compositional matrix adjust. Identities = 165/471 (35%), Positives = 246/471 (52%), Gaps = 19/471 (4%) Query: 7 KLKDGLPLHQKMRIGGKLVGSDR--VIEVRNPYNGKLVGTIPKATVGDIRDAFRIGCQFK 64 +LKD L Q+ + G+ +D+ + V NP NG+L+G +P + R A Sbjct: 3 QLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAW 62 Query: 65 SSL---TRFERSKIMLRASELLRANTDAISDLITAESGLCKKDSLYEVGRACDVFVFAAN 121 + T ERS I+ R EL+ ANTD ++ ++TAE G ++ E+G A Sbjct: 63 PAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFI----- 117 Query: 122 QTLVDDGQVFSCDLTPH-GKQRKVYTMKEPLLGAISAITPFNHPLNQVAHKVAPSIATNN 180 + ++G+ D P ++ +KE + G +AITP+N P + K P++A Sbjct: 118 EWFAEEGKRTYGDTIPSPSPSNRIVVIKEAI-GVCAAITPWNFPAAMITRKAGPALAAGC 176 Query: 181 RMVLKPTEKTPFSALLLADILYEAGLPPEMFSVVTGDPKEIADEMLTNADVDLVTFTGGV 240 MVLKP E TPFSAL LA + AG+P +FSVVTG PK I EM +N V ++FTG Sbjct: 177 PMVLKPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGST 236 Query: 241 PIGKYIAGKAV--YKRQVLELGGNDPIIVMEDADIEEAATLAVSGSYKNSGQRCTAIKRM 298 +GK + ++ K+ LELGGN P IV +DAD++ A A++ Y+N+GQ C R+ Sbjct: 237 GVGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRI 296 Query: 299 IVHQAVADEFVDKLVAKTRAIKFGNPMDAGNDMGTVIDERAAMEFEAKVKDAERCGAKLL 358 V V D F KLV + K G + G G +I+E+A + E V DA GA++L Sbjct: 297 YVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVL 356 Query: 359 YGNVRDG---ALYSPTVLDHVPADCKLVAQETFGPVSPVIRCTDIADAIRISNSTPYGLS 415 G R + + PTVL V ++ +ETFGP++P+ R + + ++N T +GL+ Sbjct: 357 LGGKRHALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLA 416 Query: 416 SSVCTNRMDYITRFVRELEVGSVNVREVPGYRLELTPFGGIKDSGLGYKEG 466 S + + I R LE G V + E+ PFGG+K SGLG +EG Sbjct: 417 SYFYSRDIGRIWRVAEGLESGMVGIN-TGLISNEVAPFGGVKQSGLG-REG 465 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 240 bits (613), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 152/460 (33%), Positives = 246/460 (53%), Gaps = 15/460 (3%) Query: 27 SDRVIEVRNPYNGKLVGTIPKATVGDIRDAFRI---GCQFKSSLTRFERSKIMLRASELL 83 S R I+V NP +++G I A D+ A G + +++ FERSKIM RA++LL Sbjct: 18 SGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERSKIMRRAADLL 77 Query: 84 RANTDAISDLITAESGLCKKDSLYEVGRACDVFVFAANQTLVDDGQVFSCDLTPHGKQRK 143 R + ++ L+T E G ++ E A D + A + G++ + P Q Sbjct: 78 RERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPARV-PGAYQ-- 134 Query: 144 VYTMKEPLLGAISAITPFNHPLNQVAHKVAPSIATNNRMVLKPTEKTPFSALLLADILYE 203 +KEP+ G ++A TP+N PLNQV K++ ++A +++K E+TP S L + Sbjct: 135 -MVIKEPV-GPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAYAD 192 Query: 204 AGLPPEMFSVVTGDPKEIADEMLTNADVDLVTFTGGVPIGKYIAGKA--VYKRQVLELGG 261 AG+P + ++V G P EI++ ++ + + ++FTG P+GK +A A KR +ELGG Sbjct: 193 AGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRATMELGG 252 Query: 262 NDPIIVMEDADIEEAATLAVSGSYKNSGQRCTAIKRMIVHQAVADEFVDKLVAKTRAIKF 321 + P +V +DAD++ AA + + ++N+GQ C + R +V + V + FVDK V +K Sbjct: 253 HAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTYAEQLKV 312 Query: 322 GNPMDAGNDMGTVIDERAAMEFEAKVKDAERCGAKLLYGNVR---DGALYSPTVLDHVPA 378 GN ++AG MG V ++R EA + DA GA+L G R G + PTVL ++P Sbjct: 313 GNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTVLTNLPL 372 Query: 379 DCKLVAQETFGPVSPVIRCTDIADAIRISNSTPYGLSSSVCTNRMDYITRFVRELEVGSV 438 + + +E FGP++ + + + I +N PYGL++ +E G + Sbjct: 373 QARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAARVESGML 432 Query: 439 NVREVPGYRLELTPFGGIKDSGLGYKEGVLEAMKSFSNTK 478 + + G L PFGGIKDSG G EG EA++++ NTK Sbjct: 433 TINHL-GLALPELPFGGIKDSGYG-SEGGTEAIEAYINTK 470 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 219 bits (557), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 153/486 (31%), Positives = 252/486 (51%), Gaps = 29/486 (5%) Query: 15 HQKMRIGGKLVGSD-------RVIEVRNPYNGKLVGTIPKATVGDIRDAFRIGCQFKSSL 67 Q + G +L+G+D R ++V +P G++ ++P D R A ++ Sbjct: 8 RQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAW 67 Query: 68 TRF---ERSKIMLRASELLRANTDAISDLITAESGLCKKDSLYEVGRACDVFVFAANQTL 124 +R+ I+ R ++LL A+ D + LI+ E G ++ EV A + + Sbjct: 68 RATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEAT 127 Query: 125 VDDGQVFSCDLTPHGKQRKVYTMKEPLLGAISAITPFNHPLNQVAHKVAPSIATNNRMVL 184 +G + +T R++ +KEP+ G ++AITP+N P +A K+AP++A +V Sbjct: 128 RANGDIIPAPVT----GRRMMALKEPV-GVVAAITPWNFPAAMIARKIAPALAAGCTVVC 182 Query: 185 KPTEKTPFSALLLADILYEAGLPPEMFSVVTGDPK---EIADEMLTNADVDLVTFTGGVP 241 KP E TP ++L L + EAG+P + ++VT + E+ D L + V ++FTG Sbjct: 183 KPAEDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTA 242 Query: 242 IGKYIAGKAV--YKRQVLELGGNDPIIVMEDADIEEAATLAVSGSYKNSGQRCTAIKRMI 299 +GK++A + K+ LELGGN P IV +DAD++ A ++ ++N GQ C + R+ Sbjct: 243 VGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIY 302 Query: 300 VHQAVADEFVDKLVAKTRAIKFGNPMDAGNDMGTVIDERAAMEFEAKVKDAERCGAKLLY 359 V + V D FVDKL A+ A+K G D + +G +I+ RA + + V+DA GA+++ Sbjct: 303 VQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVIT 362 Query: 360 GNVR-------DGALYSPTVLDHVPADCKLVAQETFGPVSPVIRCTDIADAIRISNSTPY 412 G R Y+PTVL V + +ETFGPV+P+ R + I +N+TP+ Sbjct: 363 GGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPF 422 Query: 413 GLSSSVCTNRMDYITRFVRELEVGSVNVREVPGYRLELTPFGGIKDSGLGYKEGVLEAMK 472 GL++ + + I R LE G V V E E PFGG+K+SG G +EG + + Sbjct: 423 GLAAYFYSTDVRRIHRVTDALESGIVGVNE-GALAAEAAPFGGVKESGYG-REGSVHGLD 480 Query: 473 SFSNTK 478 + +TK Sbjct: 481 DYLHTK 486 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 204 bits (519), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 153/475 (32%), Positives = 253/475 (53%), Gaps = 17/475 (3%) Query: 17 KMRIGGKLVGSD--RVIEVRNPYNGKLVGTIPKATVGDIRDAFRIG---CQFKSSLTRFE 71 ++ I G+ G + R EVR+P G ++G +P A DI +A + Q ++ E Sbjct: 6 QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 Query: 72 RSKIMLRASELLRANTDAISDLITAESGLCKKDSLYEVGRACDVFVFAANQTLVDDGQVF 131 RS I+ RA+ L R A++ IT ++G D++ E+ A + + A + G+V Sbjct: 66 RSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVV 125 Query: 132 SCDLTPHGKQRKVYTMKEPLLGAISAITPFNHPLNQVAHKVAPSIATNNRMVLKPTEKTP 191 H + R++ ++EP+ G +AI+P+N P Q KVA ++A +VLK ++P Sbjct: 126 GAR---HPEVRQL-VVREPV-GVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 Query: 192 FSALLLADILYEAGLPPEMFSVVTGDPKEIADEMLTNADVDLVTFTGGVPIGKYIAGKAV 251 + +L+A + ++AG+P +F++V+G EI+ ++ + V ++FTG VP+G +A +A Sbjct: 181 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAA 240 Query: 252 Y--KRQVLELGGNDPIIVMEDADIEEAATLAVSGSYKNSGQRCTAIKRMIVHQAVADEFV 309 KR +ELGG+ P++V +DA++E AA L +N+GQ C + R V + + FV Sbjct: 241 LHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFV 300 Query: 310 DKLVAKTRAIKFGNPMDAGNDMGTVIDERAAMEFEAKVKDAERCGAKLLYGNVR---DGA 366 IK G + G MG +I R + + DA G++L+ G R G Sbjct: 301 TAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGH 360 Query: 367 LYSPTVLDHVPADCKLVAQETFGPVSPVIRCTDIADAIRISNSTPYGLSSSVCTNRMDYI 426 Y+PT++ VP +++ +E FGP++ V + +AI ++N+ P GL+S V T +D Sbjct: 361 FYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTA 420 Query: 427 TRFVRELEVGSVNVREVPGYRLELTPFGGIKDSGLGYKEGVLEAMKSFSNTKTYS 481 R R+L+ G VN+ EL PFGGI +SG G + GV E +SF TK S Sbjct: 421 HRVSRDLQAGMVNINHSGMAHPEL-PFGGIGESGFGSEGGV-EGFESFLVTKMIS 473 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 201 bits (510), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 145/457 (31%), Positives = 220/457 (48%), Gaps = 20/457 (4%) Query: 20 IGGKLVG--SDRVIEVRNPYNGKLVGTIPKATVGDIRDAFRIGCQFKSSLTR---FERSK 74 IGG+ V S R +V NP G V + T ++ A + G + + R++ Sbjct: 8 IGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNTPPLTRAR 67 Query: 75 IMLRASELLRANTDAISDLITAESGLCKKDSLYEVGRACDVFVFAANQTLVDDGQVFSCD 134 ++ R L++ D + +I E G D+ EV R ++ FAA + G+ D Sbjct: 68 VLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEY--TD 125 Query: 135 LTPHGKQRKVYTMKEPLLGAISAITPFNHPLNQVAHKVAPSIATNNRMVLKPTEKTPFSA 194 G ++M++ L G ++ ITPFN P +IA N VLKP+E+ P ++ Sbjct: 126 QIARGID--AWSMRQAL-GVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSAS 182 Query: 195 LLLADILYEAGLPPEMFSVVTGDPKEIADEMLTNADVDLVTFTGGVPIGKYIAGKAVYK- 253 LL+AD+L EAGLP +F+VV GD K D +L + V ++F G PI +YI + K Sbjct: 183 LLIADLLKEAGLPDGVFNVVQGD-KVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKG 241 Query: 254 RQVLELGG-NDPIIVMEDADIEEAATLAVSGSYKNSGQRCTAIKRMIVHQAVADEFVDKL 312 ++V LGG + ++VM DAD++ A + +Y ++G+RC AI + + DE V L Sbjct: 242 KRVQALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGSAGDELVAAL 301 Query: 313 VAKTRAIKFGNPMDAGNDMGTVIDERAAMEFEAKVKDAERCGAKLLYGN-------VRDG 365 + R +K M AG +MG V+ A E + GA L+ G Sbjct: 302 AERARKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEKG 361 Query: 366 ALYSPTVLDHVPADCKLVAQETFGPVSPVIRCTDIADAIRISNSTPYGLSSSVCTNRMDY 425 T+LDHV + + +E FGPV V+RC DIA A+ + N+ YG +V T Sbjct: 362 FFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGGV 421 Query: 426 ITRFVRELEVGSVNVREVPGYRLELTPFGGIKDSGLG 462 FVR++EVG V V + + FGG K S G Sbjct: 422 AREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFG 458 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,466,128 Number of extensions: 61709 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 19 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)