BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0347.p (192 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 292 1e-80 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 87 1e-18 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 84 7e-18 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 78 5e-16 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 74 6e-15 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 292 bits (747), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 151/241 (62%), Positives = 172/241 (71%), Gaps = 49/241 (20%) Query: 1 MTFTPNLFQGKTALVTGGTQGIGGAVARQLAAMGAKVIAAGLPPFATEEFSGTGVTLAEL 60 M+F P+L +GKTALVTGGTQGIG AVARQL A+GA+VIAAGLPPFA EEF+G+ VTL E+ Sbjct: 1 MSFIPDLLKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEEFAGSEVTLVEM 60 Query: 61 NVASEDSVRNLFAGIQQLDIVINCAGIIKRGAEHEVDVFEQVIAVNLTGTMRVCSAARAL 120 NVASE+SVR AG+ QLDIV+NCAGIIKRGAE E +VFEQVIAVNLTGTMRVC+AAR L Sbjct: 61 NVASEESVRAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAAREL 120 Query: 121 V-------------------------------------------------VNAVAPGWIA 131 + VNAVAPGWIA Sbjct: 121 LRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIA 180 Query: 132 TPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGGYLV 191 TPLT+ALQ+DP R+GPIL RTPL RWG PED+G V AFLC+PAASFMTGA+VPVDGGYLV Sbjct: 181 TPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYLV 240 Query: 192 S 192 + Sbjct: 241 A 241 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 86.7 bits (213), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 80/250 (32%), Positives = 112/250 (44%), Gaps = 77/250 (30%) Query: 8 FQGKTALVTGGTQGIGGAVARQLAAMGAKVI--------AAGLPPF--ATEEFSGTGVTL 57 QG+ ALVTG ++GIG A+A +LA GAKVI AA + + A +G G L Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVL 65 Query: 58 ----AELNVASEDSVRNLFAGIQQLDIVINCAGIIK-----RGAEHEVDVFEQVIAVNLT 108 AE +VA + V+ F L I++N AGI + R + E ++ VIA NLT Sbjct: 66 NVNDAEASVALVEQVQKDFG---SLSILVNNAGITQDQLAMRMKDEE---WDSVIATNLT 119 Query: 109 GTMRVCSA--------------------------------------------------AR 118 R+ A +R Sbjct: 120 AVGRLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSR 179 Query: 119 ALVVNAVAPGWIATPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFM 178 + VN VAPG+I T +T+AL E +S ILQ+ PL R G E+I AFL +P A+++ Sbjct: 180 NITVNCVAPGFIDTDMTRALNEQ--QSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYI 237 Query: 179 TGAVVPVDGG 188 TG + V+GG Sbjct: 238 TGTTLNVNGG 247 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 84.0 bits (206), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 78/241 (32%), Positives = 105/241 (43%), Gaps = 62/241 (25%) Query: 8 FQGKTALVTGGTQGIGGAVARQLAAMGAKVIAAGLPPFATE-----EFSGTGVTLAELNV 62 F GKT ++TG +GIG A A LA GA+VIA L A++ E G V +L+ Sbjct: 3 FAGKTVIITGAGRGIGRACALLLAQRGAQVIA--LARTASDLARLQEEIGARVIAVDLSD 60 Query: 63 ASEDSVRNLFAGIQQLDIVINCAGI--IKRGAEHEVDVFEQVIAVNLTGTMRVCSA---- 116 + AG D +IN AGI ++ E + +E V+ VNL + C A Sbjct: 61 PAAARRAMAEAGTA--DFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARM 118 Query: 117 ------------------------------------------ARAL-----VVNAVAPGW 129 AR L VNAVAP Sbjct: 119 RIAQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTI 178 Query: 130 IATPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGGY 189 T L +A DPA+SGP++ R P R+ +PED+ A L +P A+ + GAVVPVDGG+ Sbjct: 179 TLTELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGF 238 Query: 190 L 190 L Sbjct: 239 L 239 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 77.8 bits (190), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 61/242 (25%), Positives = 113/242 (46%), Gaps = 63/242 (26%) Query: 11 KTALVTGGTQGIGGAVARQLAAMGAKVIAAGLPPFATEEFS------GTGVTLAELNVAS 64 + ALVTGG GIG ++++QLAA G V+ A + A + + G ++++ S Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 65 EDSVRNLFAGIQQ----LDIVINCAGIIKRGA--EHEVDVFEQVIAVNLTGTMRV-CSAA 117 +S+ FA I++ D+++N AG+ + + ++ ++ + Q + VN+TG + AA Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 118 RALV-------------------------------------------------VNAVAPG 128 R +V VN+VAPG Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 129 WIATPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGG 188 + TP+T+A+ + R + P+ R+GT ++I + +L ++++TG V+PVDGG Sbjct: 189 PVDTPMTQAMHSEQTRE-SYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 Query: 189 YL 190 ++ Sbjct: 248 FV 249 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 74.3 bits (181), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 70/252 (27%), Positives = 100/252 (39%), Gaps = 75/252 (29%) Query: 8 FQGKTALVTGGTQGIGGAVARQLAAMGAKVIAA------------GLPPFATEEFSGTGV 55 F+GK +VTG GIG A AR+ + GA+V+ A LPP T Sbjct: 12 FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERT-------- 63 Query: 56 TLAELNVASEDSVRNL--FA--GIQQLDIVINCAGIIKRGAEHEV--DVFEQVIAVNLTG 109 E +V+ + VR L FA QLD++++ AG+ G EV + + +V A N+ G Sbjct: 64 AARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNG 123 Query: 110 TMR----------------------------------------VCSAARALV-------- 121 VC+ RA+ Sbjct: 124 VFYGAREALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGV 183 Query: 122 -VNAVAPGWIATPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTG 180 +NAV P T +T + +DP +R L R P +I V FL +P ASF+ G Sbjct: 184 RINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNG 243 Query: 181 AVVPVDGGYLVS 192 +PVDGG + S Sbjct: 244 VNLPVDGGLMAS 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 647,514 Number of extensions: 27826 Number of successful extensions: 229 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 237 Number of HSP's successfully gapped: 13 Length of query: 192 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 112 Effective length of database: 1,241,070 Effective search space: 138999840 Effective search space used: 138999840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)