BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0346.p (429 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 771 0.0 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 202 6e-53 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 167 1e-42 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 136 3e-33 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 133 3e-32 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 771 bits (1990), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/425 (92%), Positives = 409/425 (96%), Gaps = 2/425 (0%) Query: 1 MEVQK--SSKLKRIQWVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSA 58 MEVQK S +LKRIQ VAL+FLMLAGIVNYLDRSTLSIANHSVSQEL LSAS+MGLLLSA Sbjct: 1 MEVQKEKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSA 60 Query: 59 FSLAYAFSQLPIGAMLDKFGARVMLGAGMMVWSVAQLAGGFVGSLHQFLVARVFLGLGEA 118 FSLAYAFSQLPIGAMLDKFGARVMLGAGM +WSVAQLAGGFV +L QFLVARVFLGLGEA Sbjct: 61 FSLAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEA 120 Query: 119 PQFPAGAKVVSEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIA 178 PQFPAGAKVVSEWFA+RERGGPTGIFVASSTIGPALAPPILT+LMLSFGWRMMFVIMG+ Sbjct: 121 PQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVL 180 Query: 179 GIAVAVGWYIFYRNRNEVELTPSEMLYLADGTDNDPNANAKLTWHEWKGLFKYRTTWGML 238 GIAV+VGWY+FYRNRNEV+LTPSE+LYLAD DNDPNANAKL+W EWK LF YRTTWGML Sbjct: 181 GIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGML 240 Query: 239 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 298 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR Sbjct: 241 FGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR 300 Query: 299 GMAPIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA 358 GM IRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA Sbjct: 301 GMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVA 360 Query: 359 IPRRLVASLGSLQNFGGYLGGSLAPLITGIVVDKTHSFVNALLISAAVAFAAALVYMFVV 418 IPRRLVASLGS+QNFGGYLGGSLAP+ITG+VVD+THSFVNALLISAAVAFAAALVYMFVV Sbjct: 361 IPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVV 420 Query: 419 NKPIE 423 +KPIE Sbjct: 421 DKPIE 425 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 202 bits (513), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 132/428 (30%), Positives = 218/428 (50%), Gaps = 17/428 (3%) Query: 7 SKLKRIQWVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSAFSLAYAFS 66 +K ++++ L L + VNY DR+TLSIA ++ EL L MG + SAFS AY + Sbjct: 6 AKPTHVRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLA 65 Query: 67 QLPIGAMLDKFGARVMLGAGMMVWSVAQLAGGFV------GSLHQFLVARVFLGLGEAPQ 120 QLP G +LD+FG++ + + +WSV V ++ + R +GL EAP Sbjct: 66 QLPGGWLLDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFLLRFMVGLAEAPS 125 Query: 121 FPAGAKVVSEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIAGI 180 FPA ++V+ WF +ERG + IF ++ L P++ ++ ++ W +F +MG+ GI Sbjct: 126 FPANGRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGI 185 Query: 181 AVAVGW-YIFYRNRNEVELTPSEMLYLADGTDNDPNANAKLTWHEWKG------LFKYRT 233 A+A+ W I Y ++ + +E+ ++ G N K+ KG L R Sbjct: 186 ALALIWGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRM 245 Query: 234 TWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIAD 293 G+ G + + + +LTW P YL ER +SI + G+V IP + G +G + G I+D Sbjct: 246 LLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISD 305 Query: 294 GLMKRGMAPIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGG-AW 352 L+KRG++ +RK PI G++ + I Y + M + +A+AF G W Sbjct: 306 RLIKRGLSVTWARKIPIVGGMLLSMTM-ILCNYVEAQWMVVGI--MALAFFGKGVGALGW 362 Query: 353 ALVSVAIPRRLVASLGSLQNFGGYLGGSLAPLITGIVVDKTHSFVNALLISAAVAFAAAL 412 A+V+ P+++V G L N G + G P++ G +V +T SF AL+ A + Sbjct: 363 AVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETGSFSGALVFVGINALVTVI 422 Query: 413 VYMFVVNK 420 Y+ +V + Sbjct: 423 SYLVIVKE 430 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 167 bits (424), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 113/388 (29%), Positives = 188/388 (48%), Gaps = 10/388 (2%) Query: 9 LKRIQWVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSAFSLAYAFSQL 68 ++ I+W +T + LA IVNYL R+TLS+A ++ +EL +S + ++ A+ + YA Q Sbjct: 4 VRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQP 63 Query: 69 PIGAMLDKFGARVMLGAGMMVWSVAQLAGGFVGSLHQFLVARVFLGLGEAPQFPAGAKVV 128 G +LD G ++ G + WS+ A F R LG+ EA P G K Sbjct: 64 VAGYILDAVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKAS 123 Query: 129 SEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIAGIAVAVGWYI 188 +EWF +ER G F S+IG APP++ +L GW+M FV++G G+ V W + Sbjct: 124 TEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWML 183 Query: 189 FYRN-RNEVELTPSEMLYLADGTDNDPNANAKLTWHEWKGLFKYRTTWGMLFGFMGVIYM 247 FY++ R++ L+P E Y+ +G + P K+ W + + R W + Sbjct: 184 FYKSPRDQKLLSPEERAYILEGQEKSPE---KVQRESWTKIVRSRNFWSIAIPRFLSEPA 240 Query: 248 VWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMAPIRSR 306 + W+P Y+ ERH++I +P++ +G + GY++ K ++ SR Sbjct: 241 WQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSR 300 Query: 307 KWPICAGLIGAGAFTIPAA--YTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLV 364 K L+G+ + PA + S +AI +S+ SG +++ S + V Sbjct: 301 K---LVMLVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQV 357 Query: 365 ASLGSLQNFGGYLGGSLAPLITGIVVDK 392 A+ L GYLG +L L+ GI+V + Sbjct: 358 ATATGLTGMSGYLGATLFTLLFGILVTQ 385 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 136 bits (343), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 115/454 (25%), Positives = 203/454 (44%), Gaps = 50/454 (11%) Query: 3 VQKSSKLKRIQWVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSAFSLA 62 +Q + K +WV + + V DR+ L +A + +E +S +E G L S F A Sbjct: 6 IQHTKKKTHFRWVVGALIFIVYTVAAADRANLGVALPFLRKEFVMSNTEAGALASIFLFA 65 Query: 63 YAFSQLPIGAMLDKFGARVMLGAGMMVWSVAQLAGGFVGSLHQFLVARVF---LGLGEAP 119 Y+ +QLP G +FG + M+ SV G +G+ L ++F LGL E P Sbjct: 66 YSLAQLPSGLAYTRFGIPRVFSLAMLATSVFT---GLIGTASSLLALKIFRFGLGLAEGP 122 Query: 120 QFPAG-AKVVSEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIA 178 P G ++ WF RE+G TGIF+A++ GP L PP+ V+M +GWR +F + Sbjct: 123 -LPIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIFYFFAVP 181 Query: 179 GIAVAVGWYIFYRNR--NEVELTPSEMLYLAD------GTDNDPNANAKL---------- 220 G+ ++V W + +NR ++ E+ ++ D G D DP Sbjct: 182 GVILSVVWLMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLDRIIRRS 241 Query: 221 ---TWHEWKGLFKYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIP 277 T KG+F G G+ + +V + L W+P YL + SIA G V P Sbjct: 242 KVATIETTKGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANMGMVAAAP 301 Query: 278 YIFGTLGMLSSGYIADGLMKRGMAPIRSRKWPICAGLIGAGAFT-------IPAAYTPST 330 ++ +G L G + D +++ P ++ + FT I + P Sbjct: 302 WVGAVIGNLLGGAMTDRWLEKRRKP----------AMMMSAFFTTIMMVILINSPADPLL 351 Query: 331 TMAIVYISLAMAFVNMASGGAWALVSVAIPRRLVASLGSLQNFGGYLGGSLAPLITGIVV 390 A++ ++ + + ++ + + + +++ ++ N G LGG+ APL G ++ Sbjct: 352 YGALLLLTGILLNIGYSTYMVYPMGMTS--KKVYPMACAMVNTLGQLGGACAPLAAGFLL 409 Query: 391 DKTHSFVNALLISAAVAFAAALVYMFVVNKPIEG 424 D ++S+ N + I AV + + + +P++G Sbjct: 410 D-SYSW-NHVWIFMAVCSILCFITILTIREPVQG 441 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 133 bits (334), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 95/324 (29%), Positives = 156/324 (48%), Gaps = 13/324 (4%) Query: 7 SKLKRIQWVALTFLMLAGIVNYLDRSTLSIANHSVSQELGLSASEMGLLLSAFSLAYAFS 66 +K ++W + + L I NYL R++L + ++ ELG+S + +++AF + Y Sbjct: 2 KTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIM 61 Query: 67 QLPIGAMLDKFGARVMLGAGMMVWSVAQ-LAGGFVG--SLHQFLVARVFLGLGEAPQFPA 123 Q G ++D G R+ ++WSVA L G G SL F R +GL EA P+ Sbjct: 62 QPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGF---RALMGLTEAAAIPS 118 Query: 124 GAKVVSEWFAVRERGGPTGIFVASSTIGPALAPPILTVLMLSFGWRMMFVIMGIAGIAVA 183 G K V+EWF +E+ G F + +++G LAPP++ L + +GW+ FV+ GI G A Sbjct: 119 GMKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWA 178 Query: 184 VGWYIFYRN-RNEVELTPSEMLYLADGTDNDPNA-NAKLTWHEWKGLFKYRTTWGMLFGF 241 W+ FYR+ R+ L+ SE + G +A K K + + R W + Sbjct: 179 ALWFAFYRSPRDHAALSDSERETIISGQLRPVSAVRGK---RPVKDVVRSRRFWAIALAR 235 Query: 242 MGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGMA 301 + W+P Y +ERHL + +P++ LG L+ GY++ L+ R Sbjct: 236 FFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRI 295 Query: 302 PIRSRKWPICAGLIGAGAFTIPAA 325 P+ + +C ++GA PA Sbjct: 296 PLVWSR--VCGVVLGAVMMIGPAC 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,330,473 Number of extensions: 57493 Number of successful extensions: 403 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 359 Number of HSP's successfully gapped: 33 Length of query: 429 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 341 Effective length of database: 1,203,190 Effective search space: 410287790 Effective search space used: 410287790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)