BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0002.0335.p
         (285 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312853|ref|YP_003776945.1| small-conductance mechanosensit...   438   e-124
gi|300310513|ref|YP_003774605.1| small-conductance mechanosensit...    55   8e-09

>gi|300312853|ref|YP_003776945.1| small-conductance mechanosensitive
           channel protein [Herbaspirillum seropedicae SmR1]
          Length = 276

 Score =  438 bits (1127), Expect = e-124,   Method: Compositional matrix adjust.
 Identities = 216/275 (78%), Positives = 236/275 (85%), Gaps = 2/275 (0%)

Query: 1   MQQHLLNLDTLINLYLVPFGLKVLAALVIWFVGGMLIGTFAKVVRRVLAARQLEPTLVNY 60
           M  HLLN DTLINLYLVPFGLKV+AA+VIW VGGM I T +++VRRVL ARQ E TL+NY
Sbjct: 1   MSDHLLNFDTLINLYLVPFGLKVIAAIVIWIVGGMFINTLSRIVRRVLTARQFETTLINY 60

Query: 61  AISAIHVVLRVLLVMGILEVCGVPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLR 120
           A SAIHV+LRVLLVMGILEVCG+PTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLR
Sbjct: 61  ASSAIHVILRVLLVMGILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLR 120

Query: 121 PFKVGDYITAAGQTGTVTDIGLVTTTMLTDNNLRVIIGNNKLFSDIITNYNVQPTRRVDL 180
           PFKVGDYITAAGQTGTV+DIGLVTT + TDNNLRVIIGNNKLFS+ I NYNV  TRR DL
Sbjct: 121 PFKVGDYITAAGQTGTVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDL 180

Query: 181 RCQIAYGVDPADAIARLSATVAQIPNVLVDPAPAVVIQEFNATGTLLAVRLFAPTPNFGQ 240
           RCQIAY VDP  AIA+LS  +  IPNVL  PAP +VIQEFNATGTL+A+R+ APTPNFGQ
Sbjct: 181 RCQIAYSVDPQQAIAKLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQ 240

Query: 241 VYNDTNRAIAQTCAEAGWPAPATYQVSV--ANPNQ 273
           VYND   AIA+ C + GWPAPATYQV+V  A P Q
Sbjct: 241 VYNDVQNAIAEVCLKEGWPAPATYQVAVPLAQPPQ 275


>gi|300310513|ref|YP_003774605.1| small-conductance mechanosensitive
           channel protein [Herbaspirillum seropedicae SmR1]
          Length = 358

 Score = 54.7 bits (130), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 44/199 (22%), Positives = 85/199 (42%), Gaps = 11/199 (5%)

Query: 78  LEVCGVPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDYITAAGQTGTV 137
           L + G+  T  +   GA+GV +G+    + S++ +G  +++ R   +GD +      G V
Sbjct: 135 LSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKYFGKV 194

Query: 138 TDIGLVTTTMLTDNNLRVIIGNNKLFSDIITNYNV-QPTRRVDLRCQIAYGVDPADAIAR 196
           T I    T +   + +  ++ N    S+ + NY++     R+  +  I Y  D    ++ 
Sbjct: 195 TQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRLSTQLTILYQDDVETVLSI 254

Query: 197 LSATVAQIPNVLVDPAPAVVIQEFNATGTLLAVRLFAPTPNFGQ--VYNDTNRAIAQTCA 254
           +      +  V    AP  ++ +  A G  L +  +   P  G+  V +D NRAI     
Sbjct: 255 MEQAALGVQRVSQQTAPQALLIKIGADGLDLELGFWITDPENGRLNVLSDVNRAI----- 309

Query: 255 EAGWPAPATYQVSVANPNQ 273
              W A   + + VA+  +
Sbjct: 310 ---WAAFKQHGIEVAHAKR 325


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 789,595
Number of extensions: 32869
Number of successful extensions: 169
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 169
Number of HSP's successfully gapped: 2
Length of query: 285
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 201
Effective length of database: 1,222,130
Effective search space: 245648130
Effective search space used: 245648130
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 130 (54.7 bits)