BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0326.p (140 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309923|ref|YP_003774015.1| membrane protein [Herbaspirillu... 172 1e-44 >gi|300309923|ref|YP_003774015.1| membrane protein [Herbaspirillum seropedicae SmR1] Length = 138 Score = 172 bits (435), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 95/138 (68%), Positives = 113/138 (81%) Query: 1 MTTTILNTKADYAAFLLRVTSGLFFLAHAAMKIFVFTLPGTAAFFESLGLPGFLAYLIIL 60 M+T +NT+ADYAAFLLRVT G+FFL HA +KIFVFT GT FF SLG+PG+ AY +I Sbjct: 1 MSTVTVNTRADYAAFLLRVTMGVFFLVHAGLKIFVFTPAGTVGFFASLGVPGWFAYPVIA 60 Query: 61 LEVIGGIALVIGFYGDLLAIPLAIDLAGAIFTVHGKNGFFFSNPKGGWEYLAMWIVALIA 120 LEV+GGIALV+G+YG LA+PLA DL GAI VHGK GFFF+NPKGGWEYLA+WIV LIA Sbjct: 61 LEVVGGIALVLGYYGRWLALPLAADLLGAIVLVHGKAGFFFTNPKGGWEYLALWIVGLIA 120 Query: 121 VYLLGNGAFAVKRKSLNN 138 V+LLG+GAFA+KR+ Sbjct: 121 VFLLGDGAFALKREEPRR 138 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.146 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 392,279 Number of extensions: 13487 Number of successful extensions: 72 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 71 Number of HSP's successfully gapped: 1 Length of query: 140 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 64 Effective length of database: 1,260,010 Effective search space: 80640640 Effective search space used: 80640640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 126 (53.1 bits)