BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0313.p (412 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309906|ref|YP_003773998.1| major facilitator superfamily p... 389 e-109 gi|300313658|ref|YP_003777750.1| transport transmembrane protein... 233 3e-62 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 130 2e-31 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 101 1e-22 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 99 4e-22 >gi|300309906|ref|YP_003773998.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 389 bits (1000), Expect = e-109, Method: Compositional matrix adjust. Identities = 195/388 (50%), Positives = 267/388 (68%), Gaps = 3/388 (0%) Query: 25 RGQKIFVLVTLLFSWILANADRMAMSVSIIPITKEFALDAQAAGLVLGSFYISYALMQLA 84 R QK+ L L +W + ADR+ MS +IIPI+KEFALDAQ AG+VL +FY+SYALMQL Sbjct: 7 RAQKMICLSALFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLM 66 Query: 85 AGWLADRFGSRRVLVFCVACWSVFTSMTGLAGSFFGLIVIRLLFGLGEGGFAPASTVTIA 144 GWL+DR+GSR V+V CV WS+FT +T +A SF L++IR LFG GEG F+PAS+VT+A Sbjct: 67 GGWLSDRYGSRIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFSPASSVTVA 126 Query: 145 EAFPKPERARAKSLIIGASFLGSAVGTGAIAALIHAHGWRFAYHAFGLIGVAVAIVLWFA 204 E FP+ ERARAKS +I FLG+AVG+G +A + GWR +H +G+ VA +LW A Sbjct: 127 EVFPQQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMIVAAILWLA 186 Query: 205 VKD---SPVRPQEVRTKGLFRTLFGKMVLKKTMLIFFFSNIVYVGLISWMPTFLMKTRDI 261 ++ + R K R L + I+F ++IVY+G+ISWMP+FL+K I Sbjct: 187 LRSGMAAQQARSAARPKNQLRELVKNPTALRLTAIWFCTSIVYIGMISWMPSFLLKVYQI 246 Query: 262 DIVHVGAASAIPYLVAFAALNVVGWLLDKVGQGRDRLFMSIGAAMVIVFLGLMAVATTLP 321 D++H+G ASAIPYL+AF NVVGWLLDK G+GR++ FM GA VFL LM T + Sbjct: 247 DLLHIGIASAIPYLLAFLGTNVVGWLLDKHGKGREKYFMMGGALACAVFLALMISTTVIE 306 Query: 322 WLLAFWTLSLVGYTAVYGTVFAIPLKHLPDESVGTASGIINFGGQVAAGIAPVVIGMLVT 381 L+ +WTL L+ + VY TVF++PLKH P +G+A+G++NFGGQ+A IAPVV+G L+ Sbjct: 307 LLVVYWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLMNFGGQLAGSIAPVVMGSLIV 366 Query: 382 AGKGSFNLAFAFLLAAGLGALLIALSWK 409 A G++ AF FL+ + + + LIA++W+ Sbjct: 367 AFNGAYLAAFWFLVGSAMMSFLIAMTWR 394 >gi|300313658|ref|YP_003777750.1| transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 233 bits (593), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 134/372 (36%), Positives = 222/372 (59%), Gaps = 7/372 (1%) Query: 39 WILANADRMAMSVSIIPITKEFALDAQAAGLVLGSFYISYALMQLAAGWLADRFGSRRVL 98 W ++ DR A++ + I EF L G++L SF++ Y+L+QL GWLADR+GSR V+ Sbjct: 22 WCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGYSLLQLPGGWLADRYGSRPVI 81 Query: 99 VFCVACWSVFTSMTGLAGSFFGLIVIRLLFGLGEGGFAPASTVTIAEAFPKPERARAKSL 158 V + WS+FT +TG+A S L++IR +FG+GEG F AS +AE F + ER + SL Sbjct: 82 VISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPAASVKGVAENFSRDERPKMSSL 141 Query: 159 IIGASFLGSAVGTGAIAALIHAHGWRFAYHAFGLIGVAVAIVLWFAVKDSPVRP----QE 214 ++ ++++GS + IA LI +GWR +H G+ GV A+V WF V+ PVRP Sbjct: 142 LMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVFALVYWFCVR--PVRPGVGGAG 199 Query: 215 VRTKGLFRTLFGKMVLKKTMLIFFFSNIVYVGLISWMPTFLMKTRDIDIVHVGAASAIPY 274 K F L ++ + + ++F +I+ GL +WMPT+LM R +++ VG +PY Sbjct: 200 AINKQAFMALMKMPLMWQIVAVWFGLSIINKGLDTWMPTYLMTVRGLNLKAVGLLLPLPY 259 Query: 275 LVAFAALNVVGWLLDKVGQGRDRLFMSIGAAMVIVFLGLMAVATTLPWLLAFWTLSLVGY 334 ++A + + GW++ + GR++L + + + VF+ M A ++ ++A+ L+ Sbjct: 260 IMAGLSTAIGGWVMVRFFDGREKLLLIGSSLLTAVFVYCMYSAASVEGVIAWQCLAYFFK 319 Query: 335 TAVYGTVFAIPLKHLPDESVGTASGIINFGGQVAAGIAPVVIGMLVTAGKGSFNLAFAFL 394 + V T A+P K L + VG+ G++N GGQ+A I+P+VIG LV+A +++ AF+FL Sbjct: 320 SFVLATCIALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLVIGFLVSA-FANYDYAFSFL 378 Query: 395 LAAGLGALLIAL 406 + A ++L++L Sbjct: 379 IGAAFFSVLVSL 390 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 130 bits (327), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 112/423 (26%), Positives = 198/423 (46%), Gaps = 55/423 (13%) Query: 30 FVLVTLLFSWILAN-ADRMAMSVSIIPITKEFALDAQAAGLVLGSFYISYALMQLAAGWL 88 ++++ LLF N ADR +S++ + E +LD G + +F +Y L QL GWL Sbjct: 13 YLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGGWL 72 Query: 89 ADRFGSRRVLVFCVACWSVFT------SMTGLAGSFFGLIVIRLLFGLGEGGFAPASTVT 142 DRFGS+++ ++ +A WSVFT + A + L ++R + GL E PA+ Sbjct: 73 LDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFLLRFMVGLAEAPSFPANGRI 132 Query: 143 IAEAFPKPERARAKSLIIGASFLGSAVGTGAIAALIHAHGWRFAYHAFGLIGVAVAIV-- 200 +A FP ER A ++ A + + + T +A ++H + W + G++G+A+A++ Sbjct: 133 VAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALALIWG 192 Query: 201 -LWFAVKDSP-----------------------VRPQEVRTKGLFRTLFGKMVLKKTMLI 236 + ++ KD P V + +G R L +L + Sbjct: 193 RIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGVYIG 252 Query: 237 FFFSNIVYVGLISWMPTFLMKTRDIDIVHVGAASAIPYLVAFAALNVVGWLLDKVGQGRD 296 + N++ ++W P +L++ R + I+ G ++IP + F VVG L+ D Sbjct: 253 QYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIG-GVVGGLIS------D 305 Query: 297 RLF---MSIGAAMVIVFLGLMAVATTL---PWLLAFWTLSLVGYTAVYGT-----VFAIP 345 RL +S+ A I +G M ++ T+ ++ A W + + A +G +A+ Sbjct: 306 RLIKRGLSVTWARKIPIVGGMLLSMTMILCNYVEAQWMVVGIMALAFFGKGVGALGWAVV 365 Query: 346 LKHLPDESVGTASGIINFGGQVAAGIAPVVIGMLVTAGKGSFNLAFAFLLAAGLGALLIA 405 P + VG + G+ N G VA P+VIG +V GSF+ A F+ G+ AL+ Sbjct: 366 ADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKE-TGSFSGALVFV---GINALVTV 421 Query: 406 LSW 408 +S+ Sbjct: 422 ISY 424 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 101/412 (24%), Positives = 183/412 (44%), Gaps = 49/412 (11%) Query: 13 QVQEPRPAARYGRGQKIFVLVTLLFSWILANADRMAMSVSIIPITKEFALDAQAAGLVLG 72 +VQ+ + + R R Q + L L+ + I+ DR +S++ +++E L A GL+L Sbjct: 2 EVQKEK-SPRLKRIQ-VVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLS 59 Query: 73 SFYISYALMQLAAGWLADRFGSRRVLVFCVACWSVFTSMTGLAGSFFGLIVIRLLFGLGE 132 +F ++YA QL G + D+FG+R +L + WSV G + +V R+ GLGE Sbjct: 60 AFSLAYAFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGE 119 Query: 133 GGFAPASTVTIAEAFPKPERARAKSLIIGASFLGSAVGTGAIAALIHAHGWRFAYHAFGL 192 PA ++E F ER + + +S +G A+ + L+ + GWR + G+ Sbjct: 120 APQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGV 179 Query: 193 IGVAVAI---VLWFAVKDSPVRPQEV--------------------RTKGLF--RTLFGK 227 +G+AV++ + + + + P EV K LF RT +G Sbjct: 180 LGIAVSVGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWG- 238 Query: 228 MVLKKTMLIFFFSNIVYVG--LISWMPTFLMKTRDIDIVHVGAASAIPYLVAFAALNVVG 285 ++F F ++Y+ ++W+P +L R + I G IPY+ + G Sbjct: 239 -------MLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSG 291 Query: 286 WLLDKVGQGRDRLFMSIGAAMVIVFLGLM-AVATTLP--------WLLAFWTLSLVGYTA 336 ++ D + R +I + + GL+ A A T+P + + +L++ Sbjct: 292 YIADGL---MKRGMPAIRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNM 348 Query: 337 VYGTVFAIPLKHLPDESVGTASGIINFGGQVAAGIAPVVIGMLVTAGKGSFN 388 G +A+ +P V + + NFGG + +APV+ G++V N Sbjct: 349 ASGGAWALVSVAIPRRLVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVN 400 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 99.4 bits (246), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 97/416 (23%), Positives = 168/416 (40%), Gaps = 47/416 (11%) Query: 8 NALASQVQEPRPAARYGRGQKIFVLVTLLFSWILANADRMAMSVSIIPITKEFALDAQAA 67 A A Q + + R+ G IF++ T+ A ADR + V++ + KEF + A Sbjct: 2 EAAAIQHTKKKTHFRWVVGALIFIVYTV------AAADRANLGVALPFLRKEFVMSNTEA 55 Query: 68 GLVLGSFYISYALMQLAAGWLADRFGSRRVLVFCVACWSVFTSMTGLAGSFFGLIVIRLL 127 G + F +Y+L QL +G RFG RV + SVFT + G A S L + R Sbjct: 56 GALASIFLFAYSLAQLPSGLAYTRFGIPRVFSLAMLATSVFTGLIGTASSLLALKIFRFG 115 Query: 128 FGLGEGGFAPASTVTIAEAFPKPERARAKSLIIGASFLGSAVGTGAIAALIHAHGWRFAY 187 GL EG T TI FP E+ + + A+ G + A +++ GWR + Sbjct: 116 LGLAEGPLPIGITSTINNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIF 175 Query: 188 HAFGLIGVAVAIVLWFAVKDSP-------------------------------------- 209 + F + GV +++V VK+ P Sbjct: 176 YFFAVPGVILSVVWLMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLD 235 Query: 210 --VRPQEVRTKGLFRTLFGKMVLKKTMLIFFFSNIVYVGLISWMPTFLMKTRDIDIVHVG 267 +R +V T + +F + L +FF + L++W+PT+L+ + I ++G Sbjct: 236 RIIRRSKVATIETTKGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANMG 295 Query: 268 AASAIPYLVAFAALNVVGWLLDKVGQGRDRLFMSIGAAMVIVFLGLMAVATTLPWLLAFW 327 +A P++ A + G + D+ + R + M + A + + ++ + P L Sbjct: 296 MVAAAPWVGAVIGNLLGGAMTDRWLEKRRKPAMMMSAFFTTIMMVILINSPADPLLYGAL 355 Query: 328 TLSLVGYTAV-YGTVFAIPLKHLPDESVGTASGIINFGGQVAAGIAPVVIGMLVTA 382 L + Y T P+ + A ++N GQ+ AP+ G L+ + Sbjct: 356 LLLTGILLNIGYSTYMVYPMGMTSKKVYPMACAMVNTLGQLGGACAPLAAGFLLDS 411 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,287,908 Number of extensions: 57854 Number of successful extensions: 470 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 424 Number of HSP's successfully gapped: 41 Length of query: 412 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 324 Effective length of database: 1,203,190 Effective search space: 389833560 Effective search space used: 389833560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)