BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0306.p (231 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311575|ref|YP_003775667.1| hypothetical protein Hsero_2260... 234 5e-63 >gi|300311575|ref|YP_003775667.1| hypothetical protein Hsero_2260 [Herbaspirillum seropedicae SmR1] Length = 287 Score = 234 bits (596), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 144/249 (57%), Positives = 169/249 (67%), Gaps = 19/249 (7%) Query: 2 TMSTPS-----PQSIHSGLWLASQLALGGARCIDTGFAALSKELPGRGWPTGALIDLLLQ 56 TM +PS P++IH LWLASQLA G R + +GF AL ELPGRGWPTG LI+LLL Sbjct: 39 TMLSPSSPARTPETIHPALWLASQLARGNGRGVSSGFEALDAELPGRGWPTGVLIELLLP 98 Query: 57 QPGVGELRLLGPAL---QALAP----HRIALVQTPHAPQIIGWHQNGVDASRLLSVRTAT 109 Q GVGELRLL P L Q L P RIAL+Q PHA GW GV A+RLL ++ T Sbjct: 99 QDGVGELRLLRPLLAQPQRLRPGAPSGRIALLQAPHAINAPGWAAMGVPATRLLQLQADT 158 Query: 110 SADAAWAAEQILKSGSCAALLLWQRHLRK---DSLRRLHLAAQSGNTLFVMLRPLSCAQD 166 DA WAAEQ+L++ SC ALL+WQ LRK D LRRLH+AAQ+G TLF MLRPL+CA++ Sbjct: 159 PVDACWAAEQVLRADSCQALLMWQARLRKGDHDLLRRLHVAAQAGQTLFFMLRPLACARE 218 Query: 167 ASPXG--GGGGPRGPGIDIGFIKRRGPQRDEPLFISL-PSLNLLHH-APVDRRTPAPAAV 222 ASP P G+DIGF+KRRGP R+ LF+ L PS NL H +PVDRRTPA AA Sbjct: 219 ASPAPLRLTLRPAPGGLDIGFLKRRGPLREHSLFLPLTPSPNLQRHVSPVDRRTPATAAA 278 Query: 223 GSVLSELVG 231 GS+ SEL G Sbjct: 279 GSLPSELAG 287 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 730,693 Number of extensions: 28702 Number of successful extensions: 96 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 1 Length of query: 231 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 149 Effective length of database: 1,231,600 Effective search space: 183508400 Effective search space used: 183508400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)