BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0295.p (525 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 833 0.0 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 224 1e-59 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 210 2e-55 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 113 4e-26 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 108 9e-25 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 833 bits (2152), Expect = 0.0, Method: Compositional matrix adjust. Identities = 446/508 (87%), Positives = 483/508 (95%) Query: 18 SGRKADASAWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEI 77 +G KADA+AWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEI Sbjct: 7 NGEKADAAAWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEI 66 Query: 78 IIIPLSSWLEKLLGLRTLLLAAAGLFTLFSVMCGLSTTLPMMIVGRIGQGFTGGALIPTA 137 +IIP+S+WLE+LLGLR LLL AA LFTLFSV+CGLSTTLPMMIVGR+GQGFTGGALIPTA Sbjct: 67 VIIPMSAWLERLLGLRVLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTA 126 Query: 138 MTIIATRLPREQQPVGNALFGATAILGPVLGPVLGGWLTENVSWHYSFFLNVPVGIALIT 197 MTIIATRLPR QQPVGNA+FGATAILGPVLGPV+GGWLTENVSWHY+FF+N+PVG ALIT Sbjct: 127 MTIIATRLPRSQQPVGNAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALIT 186 Query: 198 LLLVAVPYERPKVELLAEADWLGILGMALGLGGLTVVLEEGEREQWFSSPDIRWLAVVSL 257 LLLVA+PYERPK E LAEADW GI G+ALGLGGLTVVLEEGEREQWFSSPDIRWLA S+ Sbjct: 187 LLLVAIPYERPKFEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSV 246 Query: 258 LGFILLAIGQWRARHPVIQLRLLRDRQFGSVALMAMAVGVALYGTAYVIPQFLAGIAGYN 317 LGFILLAIGQ RARHPVIQLRLLRDRQFGSV +MAM VGVALYGTAYVIPQFL+GIAGYN Sbjct: 247 LGFILLAIGQVRARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYN 306 Query: 318 SLQSGVIVLLSGVPMILMMPFTPLLMRTVDIRLAVGVGLLLLAASAFMETDLSPLSYGGS 377 SLQSG IVLLSGVPMILMMPFTPLLMR VDIR+AVG G LLLAASAF+ETDLSPLSYGGS Sbjct: 307 SLQSGWIVLLSGVPMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYGGS 366 Query: 378 FVASQLMRGIGTVLAMLFLNQAAIRSVPASQAGDAAGIYSAARNLGGSLALASIAVIQDQ 437 FVASQLMRG+GTVLAMLFLNQAAIRSVP SQAGDA+G+YSAARNLGGSLALA+IAVIQDQ Sbjct: 367 FVASQLMRGVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQ 426 Query: 438 RLWLHSRRLEESISANAELVQSSMATQAHLLGGPDIAIRLLGSTIQMQALTMTYADLFWL 497 RLWLHSRRLEES++AN++LVQSSM Q HLLGG D+A++L+G+TIQ QALTMTYADLFW+ Sbjct: 427 RLWLHSRRLEESLNANSDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTYADLFWM 486 Query: 498 LGVAILCITPLVLFLRPLPTNAAPVAAH 525 LGVAILC+TPLVLFLRPLPT+AAPVA+H Sbjct: 487 LGVAILCVTPLVLFLRPLPTHAAPVASH 514 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 224 bits (571), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 156/497 (31%), Positives = 258/497 (51%), Gaps = 23/497 (4%) Query: 38 LMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIIIIPLSSWLEKLLGLRTLLL 97 +A LD +I N S+ TI G +GAS ++GT++ T+Y VAE I +PL+ WL G + + Sbjct: 43 FIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFI 102 Query: 98 AAAGLFTLFSVMCGLSTTLPMMIVGRIGQGFTGGALIPTAMTIIATRLPREQQPVGNALF 157 + +F LFS +CG++ +L M+I R+ QG GG L+P + T++ P+E+ P L+ Sbjct: 103 LSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLW 162 Query: 158 GATAILGPVLGPVLGGWLTENVSWHYSFFLNVPVGIALITLLL---VAVPYERPKVELLA 214 T ++ P+ GP+LGG L + SW Y F++NVPV ALI + + YE +L Sbjct: 163 AMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPV--ALICGYIGWRMLKRYETKMSKL-- 218 Query: 215 EADWLGILGMALGLGGLTVVLEEGEREQWFSSPDIRWLAVVSLLGFILLAIGQWRARHPV 274 D +G++ M + + L ++L+EG+ + WF+S +I LAV +++ FI I + R+P+ Sbjct: 219 PIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPI 278 Query: 275 IQLRLLRDRQF-GSVALMAMAVGVALYGTAYVIPQFLAGIAGYNSLQSGVIVLLSGVPMI 333 + LR+ R R F SV + +A G A +G+ + P +L G GY + SG I L+G+ + Sbjct: 279 VDLRVFRHRGFTASVTTICLAFG-AFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAV 337 Query: 334 LMMPFTPLLMRTVDIRLAVGVGLLLLAA----SAFMETDLSPLSYGGSFVASQLMRGIGT 389 + P + T+D R V +G+ L AF TD+S G ++GIG Sbjct: 338 MTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPL----FLQGIGL 393 Query: 390 VLAMLFLNQAAIRSVPASQAGDAAGIYSAARNLGGSLALASIAVIQDQRLWLHSRRLEES 449 L + L A+ SV + AAG+ S R + G++A + + + L Sbjct: 394 PLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGL 453 Query: 450 ISANAELVQSSMATQAHLLGGPDIAIRLLGSTIQMQALTMTYADLFWLLGVAILCITPLV 509 + + + + MA+ + A L T+Q QA+ ++ +F + V+ + L Sbjct: 454 VDRGGDAIHTMMASGMS----AEQATASLAQTVQNQAVMLSTNHIFMMAAVS-FSLAALA 508 Query: 510 LFLRPLPTNAAPV-AAH 525 ++L P PT A AAH Sbjct: 509 VWLAPKPTRVADTSAAH 525 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 210 bits (534), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 136/470 (28%), Positives = 238/470 (50%), Gaps = 6/470 (1%) Query: 34 TLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIIIIPLSSWLEKLLGLR 93 +L M LD SI N ++PTI G +G S EGTW+ T++ A I IPL+ WL + LG Sbjct: 30 SLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAV 89 Query: 94 TLLLAAAGLFTLFSVMCGLSTTLPMMIVGRIGQGFTGGALIPTAMTIIATRLPREQQPVG 153 L + + LF L S +CGL+ TLP+++ R+ QG G ++P + +++ P+ + Sbjct: 90 RLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFA 149 Query: 154 NALFGATAILGPVLGPVLGGWLTENVSWHYSFFLNVPVG-IALITLLLVAVPYERPKVEL 212 AL+G TA++ PV GP LGGW+T++ +W + F++N+PVG IA L + E P +L Sbjct: 150 LALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKL 209 Query: 213 LAEADWLGILGMALGLGGLTVVLEEGEREQWFSSPDIRWLAVVSLLGFILLAIGQWRARH 272 D +G+ + + ++L++G+ WF+SP I L +++L+ F + + H Sbjct: 210 --PIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNNDH 267 Query: 273 PVIQLRLLRDRQF-GSVALMAMAVGVALYGTAYVIPQFLAGIAGYNSLQSGVIVLLSGVP 331 PV+ LRL R F G +++ GV +G ++PQ+L GY S+ SG+ G+ Sbjct: 268 PVVDLRLFGRRNFAGGTIAISIGYGV-FFGNLVILPQWLQQYLGYRSIDSGLSTAPIGIF 326 Query: 332 MILMMPFTPLLMRTVDIRLAVGVGLLLLAASAFMETDLSPLSYGGSFVASQLMRGIGTVL 391 +++MP + VD R + A ++ + + + + L++GI T + Sbjct: 327 AVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTAM 386 Query: 392 AMLFLNQAAIRSVPASQAGDAAGIYSAARNLGGSLALASIAVIQDQRLWLHSRRLEESIS 451 + L + +P + AAG+ S R G++ + + R +H +L E + Sbjct: 387 FFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRSIMHHAQLTEHAT 446 Query: 452 ANAELVQSSMATQAHLLG-GPDIAIRLLGSTIQMQALTMTYADLFWLLGV 500 + +Q ++ + LG + +L TI QA + D+F++ V Sbjct: 447 PYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAV 496 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 113 bits (282), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 64/182 (35%), Positives = 99/182 (54%), Gaps = 4/182 (2%) Query: 36 GALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIIIIPLSSWLEKLLGLRTL 95 G M TLD +IVN++LP + +G + AY + + P S WL G R + Sbjct: 61 GFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRM 120 Query: 96 LLAAAGLFTLFSVMCGLSTTLPMMIVGRIGQGFTGGALIPTAMTIIATRLPREQQPVGNA 155 AA LF+L S++C ++++LPM++ R+ QG G L+P + +P Q A Sbjct: 121 YFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALA 180 Query: 156 LFGATAILGPVLGPVLGGWLTENVSWHYSFFLNVPVGIALITLLLVAVPYER----PKVE 211 LGPV+GPVLGGWL E++SWH+ F +N+PVG+ + +L +P + P+ + Sbjct: 181 SISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFD 240 Query: 212 LL 213 LL Sbjct: 241 LL 242 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 108 bits (271), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 85/311 (27%), Positives = 140/311 (45%), Gaps = 9/311 (2%) Query: 42 LDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIIIIPLSSWLEKLLGLRTLLLAAAG 101 +D +++++SLP + ++ T+YLV+ + IP+S W+ G T+ +A Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 102 LFTLFSVMCGLSTTLPMMIVGRIGQGFTGGALIPTAMTIIATRLPREQQPVGNALFGATA 161 +F L S++CG+S+TLP + R QG G ++P +I + + + A Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 162 ILGPVLGPVLGGWLTENVSWHYSFFLNVPVGIALITLLLVAVPYERPKVELLAEADWLGI 221 + GPVLGP LGG++T W + FF+N+P+ I + L +P K E +A D G Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIP--DLKAEQVAPLDLKGF 178 Query: 222 LGMALGLGGLTVVLEEGEREQWFSSPDIRWLAVVSLLGFILLAIGQWRARH---PVIQLR 278 L ALG L + L R S ++ V++ G + LA W AR P+I L Sbjct: 179 LLSALGCSLLMMGLATAGRH--LVSGEVALTCVLA--GLVFLAAYVWHARRASAPLIDLA 234 Query: 279 LLRDRQFGSVALMAMAVGVALYGTAYVIPQFLAGIAGYNSLQSGVIVLLSGVPMILMMPF 338 LL+ + V + +++P L GY SG++ S + M Sbjct: 235 LLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTI 294 Query: 339 TPLLMRTVDIR 349 +R R Sbjct: 295 AARFLRRYGFR 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,339,577 Number of extensions: 46545 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 273 Number of HSP's successfully gapped: 15 Length of query: 525 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 435 Effective length of database: 1,193,720 Effective search space: 519268200 Effective search space used: 519268200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)