BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0294.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbasp... 645 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 159 5e-40 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 142 5e-35 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 141 1e-34 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 136 4e-33 >gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 645 bits (1663), Expect = 0.0, Method: Compositional matrix adjust. Identities = 342/455 (75%), Positives = 388/455 (85%), Gaps = 15/455 (3%) Query: 2 QSGRFVRAQDAASAEAPVNRWWEALGDPVLNDLVQRALDANPNLGIARAXALDANPNLGI 61 G+FVR D+A+ AP+N+WW+ LGD L L++RAL ANPNLGIARA L Sbjct: 46 HGGQFVRGGDSATTTAPINQWWQGLGDAQLTSLIERALQANPNLGIARA-------RLQQ 98 Query: 62 ARAALNLEQANALPSVGASALAGHARLPPINLGS-SSGASTSSGSSTSLNLYSLGFDATW 120 +RA L LEQANA PSVGASALAGHARLPP NLG+ + GAS+ +GS TS NLYSLGFDA+W Sbjct: 99 SRAGLALEQANAAPSVGASALAGHARLPPANLGALTGGASSGNGSPTSANLYSLGFDASW 158 Query: 121 EADLFGGHRRAVQAANATAEAAEASLADVQVSLEAEVANAYINLRDRQRRIRLADESIRA 180 E DLFGG RRAVQAA ATA+AA+ASLADVQVSL AEVANAYI+LRDRQ+R+ L ++++A Sbjct: 159 EIDLFGGRRRAVQAATATADAAQASLADVQVSLAAEVANAYISLRDRQQRLALGQQAVQA 218 Query: 181 QEQMATLTQQRLERGTASNLDLIRVRNQLDGTRSDQVPLQADLESYLNQLATLVGQEPGT 240 QEQM LT QR+ERGTAS+LD +R++NQLD +R+D VPLQAD ++YLNQLATL+GQEPGT Sbjct: 219 QEQMLQLTGQRVERGTASSLDQLRIQNQLDASRADLVPLQADRDAYLNQLATLLGQEPGT 278 Query: 241 LDEMLVANANAENAENAVPLPPAQVAAGDPQALLRRRPDIRAAERTLAADTAKIGQAEAA 300 LD L A VPLPP QVA GDP +LLRRRPD+RAAERTLAADTAKIGQAEAA Sbjct: 279 LDGELAATV-------PVPLPPQQVAVGDPVSLLRRRPDVRAAERTLAADTAKIGQAEAA 331 Query: 301 RYPSLKLFGVIGLGGTHPSDLTKLDDFTAVAAPMLSWNFLDFGRAKARVTQAERVRDEAE 360 RYPSLKLFGVIGLGGTHPSDLT+LDDF A+ APMLSWNFLDFGRAKARVTQAERVRDEAE Sbjct: 332 RYPSLKLFGVIGLGGTHPSDLTRLDDFAALGAPMLSWNFLDFGRAKARVTQAERVRDEAE 391 Query: 361 IKYRQSVLEALRDAEDSLSRFRYGRVTVATVARTKASADRALDLAQQRYRAGTGTLIDVL 420 +KYRQSVLEALRDAED+LSRFRYGRV+VAT+ARTK SADRAL LA+QRY+AGTGTLI+VL Sbjct: 392 MKYRQSVLEALRDAEDALSRFRYGRVSVATIARTKLSADRALALARQRYQAGTGTLIEVL 451 Query: 421 DTTRQQLSAQQNLSQAEAALTRSFVGIQKALGLGW 455 DTTRQQ++AQQNL QAEA LTRSFVGIQKALGLGW Sbjct: 452 DTTRQQITAQQNLLQAEANLTRSFVGIQKALGLGW 486 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 159 bits (402), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 151/453 (33%), Positives = 224/453 (49%), Gaps = 33/453 (7%) Query: 12 AASAEAPVNR---WWEALGDPVLNDLVQRALDANPNLGIARAXALDANPNLGIARAALNL 68 A ++ APV W + DP L ++ AL+ N +L +A A G+ RAAL Sbjct: 53 AGASSAPVVADIGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAAL-- 110 Query: 69 EQANALPSVGASALAGHARLPPINLGSSSGASTSSGSSTSLNLYSLGFDATWEADLFGGH 128 PS+ A+ +R P +L SS+G++ +S S T+ +LGF A +E DLFG Sbjct: 111 -----FPSIKAATSETASRTPG-DL-SSTGSALTSRSYTA----TLGFSA-YELDLFGRV 158 Query: 129 R----RAVQAANATAEAAEASLADVQVSLEAEVANAYINLRDRQRRIRLADESIRAQEQM 184 R +A+Q+ +TAEA ++ Q+SL AEVA A++ L Q R+RLA +++ +Q Sbjct: 159 RSLKEQALQSFLSTAEARRST----QISLIAEVATAWLTLASDQDRLRLAQDTLESQSSS 214 Query: 185 ATLTQQRLERGTASNLDLIRVRNQLDGTRSDQVPLQADLESYLNQLATLVGQE-PGTLDE 243 LTQ+ E G++S L L + + ++ R D A ++ N L LVG++ P L Sbjct: 215 YALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNALVLLVGKDLPTALLP 274 Query: 244 MLVANANAENAENAVPLPPAQVAAGDPQALLRRRPDIRAAERTLAADTAKIGQAEAARYP 303 + + A +PP G P LL+RR DI AER L A A IG A AA YP Sbjct: 275 QGLPDTRLAAASPLATIPP-----GLPSDLLQRRADIVQAERDLRAANANIGAARAAFYP 329 Query: 304 SLKLFGVIGLGGTHPSDLTKLDDFTAVAAPMLSWNFLDFGRAKARVTQAERVRDEAEIKY 363 S+ L G + L K + P +S D G +A + A RD + +Y Sbjct: 330 SITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQANLDIATVSRDISVAQY 389 Query: 364 RQSVLEALRDAEDSLS-RFRYGRVTVATVARTKASADRALDLAQQRYRAGTGTLIDVLDT 422 +++ A R+ D+L+ R GR A A +AS D A L+Q RY +G + + VLD+ Sbjct: 390 EKAIQTAFREVSDALAQRNTLGRQLQAQEALVQAS-DEAFRLSQARYHSGVDSYLTVLDS 448 Query: 423 TRQQLSAQQNLSQAEAALTRSFVGIQKALGLGW 455 R SAQ+NL + + + V K LG GW Sbjct: 449 QRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGW 481 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 142 bits (358), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 144/457 (31%), Positives = 208/457 (45%), Gaps = 41/457 (8%) Query: 9 AQDAASAEAPVNRWWEALGDPVLNDLVQRALDANPNLGIARAXALDANPNLGIARAALNL 68 A+D A E WW+ GD LN L A AN +L A A LG ARA Sbjct: 70 AEDIARGE-----WWKIFGDEKLNALEDTAQQANQDLKAAAA-------RLGQARALTRD 117 Query: 69 EQANALPSVGASALAGHARLPPINLGSSSGASTSSGSSTSLNLYSLGFDATWEADLFGGH 128 +A P V A A R P + G + A T + LY + ++E DLFG Sbjct: 118 ARAGYFPQVDAGVGATRQRPSPASQGLPADADTRPNT-----LYRAQVNVSYEVDLFGRV 172 Query: 129 RRAVQAANATAEAAEASLADVQVSLEAEVANAYINLRDRQRRIRLADESIRAQEQMATLT 188 V AA A A+ +EA V ++L+A+VA Y +R+ L ++ +++ L Sbjct: 173 ASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLV 232 Query: 189 QQRLERGTASNLDLIRVRNQLDGTRSDQVPLQADLESYLNQLATLVGQEPGTLDEMLVAN 248 Q+R + G S +D+ R R +L +S+ + + + + LA L+G+ P Sbjct: 233 QRRFDEGDISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTP---------- 282 Query: 249 ANAENAENAVPLPP-----AQVAAGDPQALLRRRPDIRAAERTLAADTAKIGQAEAARYP 303 AE ++P P V G P ALL RRPDI AAER +AA A++G A++A +P Sbjct: 283 -----AEFSLPPQPLTRLAVTVPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFP 337 Query: 304 SLKLFGVIGLGGTHPSDLTKLDDFTAVAAPM----LSWNFLDFGRAKARVTQAERVRDEA 359 SL L G +G DL T + P+ LS D GR +A V +A +E Sbjct: 338 SLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQ 397 Query: 360 EIKYRQSVLEALRDAEDSLSRFRYGRVTVATVARTKASADRALDLAQQRYRAGTGTLIDV 419 YR +VL A R+ ED+L+ R A++ RA L+ +YR G + V Sbjct: 398 VASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSV 457 Query: 420 LDTTRQQLSAQQNLSQAEAALTRSFVGIQKALGLGWG 456 +D R L Q+ + RS V + +ALG GWG Sbjct: 458 IDADRTVLQQQRASVTLDGERARSTVNLIRALGGGWG 494 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 141 bits (356), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 133/445 (29%), Positives = 203/445 (45%), Gaps = 47/445 (10%) Query: 22 WWEALGDPVLNDLVQRALDANPNLGIARAXALDANPNLGIARAALNLEQANALPSVGASA 81 W + D L L+ AL+ N +L R L N+ ARA + +A+ LP++ S Sbjct: 62 WRQFFPDQRLQQLIAAALENNRDL---RTAVL----NIEEARAQYQIARADLLPNLNLSG 114 Query: 82 LAGHARLPPINLGSSSGASTSSGSSTSLNLYSLGFDA-TWEADLFGGHRRAVQAANATAE 140 A R ++ + + +G T N Y +G +E D FG R AA A+ Sbjct: 115 NAARTR--------TAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYL 166 Query: 141 AAEASLADVQVSLEAEVANAYINLRDRQRRIRLADESIRAQEQMATLTQQRLERGTASNL 200 + E + Q+SL A+VA AY+ R +++LADE++++++ L Q+RL+ G ++ L Sbjct: 167 STEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTAL 226 Query: 201 DLIRVRNQLDGTRSDQVPLQADLESYLNQLATLVGQEPGTLDEMLVANANAENAENAVPL 260 D + ++ R + L+ N L LVG++ G L Sbjct: 227 DFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGE-------------------L 267 Query: 261 PPAQ----------VAAGDPQALLRRRPDIRAAERTLAADTAKIGQAEAARYPSLKLFGV 310 PPAQ + AG P LL RPDIR+AE++L A A IG A AA +P + L G Sbjct: 268 PPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGN 327 Query: 311 IGLGGTHPSDLTKLDDFTAVAAPMLSWNFLDFGRAKARVTQAERVRDEAEIKYRQSVLEA 370 G + L + P LS DFGR A + AE +++A + Y +++ A Sbjct: 328 FGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTA 387 Query: 371 LRDAEDSL-SRFRYGRVTVATVARTKASADRALDLAQQRYRAGTGTLIDVLDTTRQQLSA 429 R+ D+L +R A A A +R L L R++ G + +D LD R SA Sbjct: 388 FREVADALVARGSLDEQVKAQEAFLAAQQER-LKLTDLRFKNGIASSLDQLDAQRDLFSA 446 Query: 430 QQNLSQAEAALTRSFVGIQKALGLG 454 QQ L QA + + + KALG G Sbjct: 447 QQALIQARQLRLNNAIDLYKALGGG 471 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 136 bits (342), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 140/445 (31%), Positives = 208/445 (46%), Gaps = 27/445 (6%) Query: 10 QDAASAEAPVNRWWEALGDPVLNDLVQRALDANPNLGIARAXALDANPNLGIARAALNLE 69 Q A A +P W + DP L L+ +AL+ + +L A +A GI A L Sbjct: 54 QVANEAASPPLPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADL--- 110 Query: 70 QANALPSVGASALAGHARLPPINLGSSSGASTSSGSSTSLNLYSLGFDATWEADLFGGHR 129 P++GA A +R P +L + S L L S WE DL+G R Sbjct: 111 ----FPTIGAQAGLDRSRTP-ADLNLTRRPLVGSAYQAGLGLSS------WEIDLWGRLR 159 Query: 130 RAVQAANATAEAAEASLADVQVSLEAEVANAYINLRDRQRRIRLADESIRAQEQMATLTQ 189 AA +A A++A+ + V +SL A+VA+A++ L + R+ +A+++I ++++ + Sbjct: 160 SLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFS 219 Query: 190 QRLERGTASNLDLIRVRNQLDGTRSDQVPLQADLESYLNQLATLVGQEPGTLDEMLVANA 249 +R+E G S L+L ++ L ++ LQ LN L LVG P L V A Sbjct: 220 RRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVG-SPVVLP---VGQA 275 Query: 250 NAENAENAVPLPPAQVAAGDPQALLRRRPDIRAAERTLAADTAKIGQAEAARYPSLKLFG 309 A PL P G P LL RRPD+ AAE L A A IG A AA +PS+ L G Sbjct: 276 RAGLDAVFAPLQP-----GLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTG 330 Query: 310 VIGLGGTHPSDLTKLDDFTAVAAPMLSWNFLDFGRAKARVTQAERVRDEAEIKYRQSVLE 369 +G S L + +P ++ GR + + +E RD A Y ++V Sbjct: 331 NVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQT 390 Query: 370 ALRDAEDSLS--RFRYGRVTVATVARTKASADRALDLAQQRYRAGTGTLIDVLDTTRQQL 427 A R+ D+L+ ++ ++ +A A + +RA L+ RY G +DVLD R L Sbjct: 391 AFREVADALAARQWLTQQLEIAQSA-VRVQRERA-RLSALRYDNGAAPFLDVLDAQRDLL 448 Query: 428 SAQQNLSQAEAALTRSFVGIQKALG 452 SAQQ L Q AL S V + ALG Sbjct: 449 SAQQQLVQIRRALLSSSVALYAALG 473 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.128 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,160,605 Number of extensions: 41952 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 18 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)