BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0287.p (191 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 309 1e-85 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 188 3e-49 gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014... 178 3e-46 gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 153 9e-39 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 149 1e-37 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 309 bits (791), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 152/186 (81%), Positives = 162/186 (87%) Query: 1 METACTQLARWALRAGTAHLSIAVNVSVQQFREADFVETVCGILERTGANPKRLKLELTE 60 +ETAC QLA WA + A LSIAVNVS Q RE DFV+TV +ERTGANP+RLKLELTE Sbjct: 846 LETACRQLATWAAQPQMADLSIAVNVSAPQLREPDFVQTVLDTIERTGANPRRLKLELTE 905 Query: 61 SLLVDNVEDIIQKMNALKARGVEFSLDDFGIGYSSLSYLKRLPLNQLKIDQSFVRDVLID 120 SLLVDNVEDIIQKM ALKA GVEFSLDDFG GYSSLSYLKRLPLNQLKIDQSFVRDVLID Sbjct: 906 SLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLID 965 Query: 121 PNDASIAKTIVALAQSLGLGVIAEGVETDAQRNFLADAGCLAYQGYFFCRPLPLDGFEQF 180 PNDASIAKTIV LAQSLGLGVIAEGVETDAQR+FLADAGC AYQGYFFCRP+ ++ FE+F Sbjct: 966 PNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFFCRPVTVEAFEKF 1025 Query: 181 VHAFDP 186 FDP Sbjct: 1026 AQEFDP 1031 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 188 bits (477), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 94/181 (51%), Positives = 126/181 (69%), Gaps = 1/181 (0%) Query: 1 METACTQLARWALRAGTAHLSIAVNVSVQQFREADFVETVCGILERTGANPKRLKLELTE 60 + ACTQ +W L G A +S++VN+SV QF + D V ++ +LE TG P+ L+LELTE Sbjct: 762 LRAACTQCRQWQL-GGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELELTE 820 Query: 61 SLLVDNVEDIIQKMNALKARGVEFSLDDFGIGYSSLSYLKRLPLNQLKIDQSFVRDVLID 120 SL++ +VE I + LK GV S+DDFG GYSSL+YLKR P++ LKID+SFVRD+ ID Sbjct: 821 SLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDITID 880 Query: 121 PNDASIAKTIVALAQSLGLGVIAEGVETDAQRNFLADAGCLAYQGYFFCRPLPLDGFEQF 180 +DA+I +I++LA+ L L VIAEGVETD Q ++L GC QGY F P+P D FEQ Sbjct: 881 ADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPADKFEQI 940 Query: 181 V 181 + Sbjct: 941 L 941 >gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014 [Herbaspirillum seropedicae SmR1] Length = 726 Score = 178 bits (451), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 85/173 (49%), Positives = 117/173 (67%) Query: 5 CTQLARWALRAGTAHLSIAVNVSVQQFREADFVETVCGILERTGANPKRLKLELTESLLV 64 CT L W L +A+NVS Q+F+ +FV I+ +K+ELTES++V Sbjct: 543 CTDLENWQREGLARDLVVAINVSAQEFKVDNFVYRFREIMREHHVPSHSVKVELTESMMV 602 Query: 65 DNVEDIIQKMNALKARGVEFSLDDFGIGYSSLSYLKRLPLNQLKIDQSFVRDVLIDPNDA 124 D++ED+I KM AL++ V SLDDFG GYSSLSYL R P++Q+KIDQ+FVR++ D A Sbjct: 603 DHIEDVITKMQALRSNEVSISLDDFGTGYSSLSYLGRFPIDQIKIDQTFVREMQNDAAMA 662 Query: 125 SIAKTIVALAQSLGLGVIAEGVETDAQRNFLADAGCLAYQGYFFCRPLPLDGF 177 SI ++I+ L QSL L +IAEGVET+ QR+FL GCL YQGY++ +P+P + F Sbjct: 663 SIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHAQGCLLYQGYWYSKPIPGEQF 715 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 153 bits (386), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 77/181 (42%), Positives = 110/181 (60%), Gaps = 1/181 (0%) Query: 1 METACTQLARWALRAGTAHLSIAVNVSVQQFREADFVETVCGILERTGANPKRLKLELTE 60 +E AC QL+ W R G SI+VN+S F + D + V G+L R P L LE+TE Sbjct: 800 LEEACFQLSDWR-RRGIQVPSISVNLSATNFHDLDLPQFVTGLLRRYALEPSDLTLEITE 858 Query: 61 SLLVDNVEDIIQKMNALKARGVEFSLDDFGIGYSSLSYLKRLPLNQLKIDQSFVRDVLID 120 ++L+D Q + A+ A G+ ++DDFG GYSSL YL++LP+++LK+DQSFVRD+ D Sbjct: 859 TVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFVRDMAKD 918 Query: 121 PNDASIAKTIVALAQSLGLGVIAEGVETDAQRNFLADAGCLAYQGYFFCRPLPLDGFEQF 180 P +++ ++ + +SL L V+AEGVE Q L GC QGY F RP+ D E + Sbjct: 919 PAVSALTNAVIRIGESLKLTVVAEGVEDAEQYALLISQGCDVAQGYLFSRPMSADALENW 978 Query: 181 V 181 V Sbjct: 979 V 979 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 149 bits (377), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 81/179 (45%), Positives = 110/179 (61%), Gaps = 1/179 (0%) Query: 1 METACTQLARWALRAGTAHLSIAVNVSVQQFREADFVETVCGILERTGANPKRLKLELTE 60 ++ AC Q+ W G +++VN+S QF + +E V LER P L LE+TE Sbjct: 508 LDEACRQMREW-FALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLPPPCLTLEVTE 566 Query: 61 SLLVDNVEDIIQKMNALKARGVEFSLDDFGIGYSSLSYLKRLPLNQLKIDQSFVRDVLID 120 S + + E +Q + + GVE S+DDFG GYSSL YLKRLP N+LKID+ FVR++ D Sbjct: 567 STAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKIDRGFVRELRQD 626 Query: 121 PNDASIAKTIVALAQSLGLGVIAEGVETDAQRNFLADAGCLAYQGYFFCRPLPLDGFEQ 179 DA+I IVAL ++L L ++AEGVET Q++FL AGC A QGY RP+ + F Q Sbjct: 627 GEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGRPMTPELFLQ 685 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 558,615 Number of extensions: 21321 Number of successful extensions: 71 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 18 Length of query: 191 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 111 Effective length of database: 1,241,070 Effective search space: 137758770 Effective search space used: 137758770 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)