BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0002.0286.p
         (237 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil...   394   e-111
gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb...   217   8e-58
gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb...    61   7e-11

>gi|300311872|ref|YP_003775964.1| glutathione S-transferase family
           protein [Herbaspirillum seropedicae SmR1]
          Length = 235

 Score =  394 bits (1011), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 187/231 (80%), Positives = 210/231 (90%), Gaps = 1/231 (0%)

Query: 8   IADFPINAKWAARHPERIQLYSLPTPNGVKASIMLEETGLAYEPHLVSFETNDQLSPEFL 67
           ++ F I+ KW A+HP+RIQLYSLPTPNGVK SIMLEETGL YE HLVSFETNDQ+SPEFL
Sbjct: 5   LSSFAIDQKWPAQHPDRIQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMSPEFL 64

Query: 68  SLNPNNKIPAIIDPDGPGGKPLALFESGAILIYLAEKTGKFLPQDPAARYQVIQWVMFQM 127
           SLNPNNKIPAIIDP+GPGG+PL LFESGAIL+YLAEKTG+FLP D AARYQVIQWVMFQM
Sbjct: 65  SLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWVMFQM 124

Query: 128 GGIGPMFGQLGFFHRFAGKEYEDKRPRDRYVAESRRLLGVLDQRLADREWV-MDEYSIAD 186
           GG+GPMFGQLGFFHRFAGKEYEDKRPRDRYV ES+RLLGV+D+RLA  +W+  D YSIAD
Sbjct: 125 GGVGPMFGQLGFFHRFAGKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAYSIAD 184

Query: 187 IAIFPWVRNLVGFYEAGELVGFQDFKNIKRALDAFVARPAVVKGLTIPARG 237
           IA FPW+RNL+GFYEA +LVGFQDF ++ RAL+AFVARPAVV+GL IPARG
Sbjct: 185 IATFPWIRNLIGFYEAADLVGFQDFPHVARALEAFVARPAVVRGLNIPARG 235


>gi|300312317|ref|YP_003776409.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 230

 Score =  217 bits (552), Expect = 8e-58,   Method: Compositional matrix adjust.
 Identities = 109/212 (51%), Positives = 142/212 (66%), Gaps = 8/212 (3%)

Query: 25  IQLYSLPTPNGVKASIMLEETGLAYEPHLVSFETNDQLSPEFLSLNPNNKIPAIIDPDGP 84
           I +YS  TPNG K  IMLEE GL Y  H +     DQ +PEFL ++PNNKIPAI+DPDGP
Sbjct: 2   IDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGP 61

Query: 85  GGKPLALFESGAILIYLAEKTGKFLPQDPAARYQVIQWVMFQMGGIGPMFGQLGFFHRFA 144
            G+P++LFESGAILIYLA KTG+FL +    +Y+V+QWVMFQMGG+GPM GQ   F  +A
Sbjct: 62  DGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLYA 121

Query: 145 GKEYEDKRPRDRYVAESRRLLGVLDQRLADREWVMDE-YSIADIAIFPWVRNLVGFYEAG 203
            ++ E     +RY  E+RRL  V+D++L    ++  E Y+IADIA FPW R+        
Sbjct: 122 PEQIE--YAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRS-----HKN 174

Query: 204 ELVGFQDFKNIKRALDAFVARPAVVKGLTIPA 235
           + +   DF N+KR  DA  ARPAV +G+T+ A
Sbjct: 175 QGIDLDDFPNVKRWYDAISARPAVQRGVTVLA 206


>gi|300310406|ref|YP_003774498.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 206

 Score = 61.2 bits (147), Expect = 7e-11,   Method: Compositional matrix adjust.
 Identities = 59/203 (29%), Positives = 91/203 (44%), Gaps = 23/203 (11%)

Query: 37  KASIMLEETGLAYEPHLVSFETNDQLSPEFLSLNPNNKIPAIIDPDGPGGKPLALFESGA 96
           +A +     GL  E   V         P FL+LNP  ++P + D +      + + +S A
Sbjct: 14  RAVLFASLLGLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGE------VVIADSNA 67

Query: 97  ILIYLAEKTGK--FLPQDPAARYQVIQWVMFQMGGI--GPMFGQL--GFFHRFAGKEYED 150
           IL+YLA+K G+  +LP+D      V +W+    G +  GP   +L   F   F  +E   
Sbjct: 68  ILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAFNAEE--- 124

Query: 151 KRPRDRYVAESRRLLGVLDQRLADREWV-MDEYSIADIAIFPWV-RNLVGFYEAGELVGF 208
                  +  +  LLG L+Q LA R+W+  D  +IAD+A++ ++ R   G  +     G 
Sbjct: 125 ------VIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGV 178

Query: 209 QDFKNIKRALDAFVARPAVVKGL 231
           Q F     AL  FV       GL
Sbjct: 179 QGFLQRIEALPGFVPFAQTAAGL 201


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.141    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 844,764
Number of extensions: 38294
Number of successful extensions: 91
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 88
Number of HSP's successfully gapped: 3
Length of query: 237
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 155
Effective length of database: 1,231,600
Effective search space: 190898000
Effective search space used: 190898000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 129 (54.3 bits)