BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0274.p (151 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311595|ref|YP_003775687.1| two component response regulato... 114 5e-27 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 78 3e-16 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 114 bits (284), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 56/136 (41%), Positives = 81/136 (59%) Query: 14 KLRDAEKAAFGYTHLDLAAAMMQDWSIPRLFCEAVLHHKAPSQSGLDEESRHLRLAQMLH 73 +LR E+ +FG+ HL LAAAMMQDW PR+FCEAVLHH+ P+ SGL+E+ R ++ ++L Sbjct: 174 QLRVQERESFGFDHLALAAAMMQDWHFPRIFCEAVLHHRDPAASGLEEDDRQQKVTRLLE 233 Query: 74 VAGIIADYCVAPEGARVQALQALLDAGAPLNGEVDHVIALCDRVGRDWMEWSSMVNISAQ 133 +AG +AD C A + R L LL G V + +C RV DW +W +++ + + Sbjct: 234 LAGCVADVCTASDAQRAALLPRLLQLGQHFALPVSSLEYVCGRVSWDWSDWGALLKVPTR 293 Query: 134 ALPPTRDVLAQLREQS 149 L LA EQ+ Sbjct: 294 VLREIGPELAAAAEQT 309 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 78.2 bits (191), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 42/116 (36%), Positives = 63/116 (54%) Query: 16 RDAEKAAFGYTHLDLAAAMMQDWSIPRLFCEAVLHHKAPSQSGLDEESRHLRLAQMLHVA 75 R E++ FG+ HL++AAA+MQDW IP LFC+AVLH++ P G +R + LH+A Sbjct: 176 RAMEQSLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLA 235 Query: 76 GIIADYCVAPEGARVQALQALLDAGAPLNGEVDHVIALCDRVGRDWMEWSSMVNIS 131 +A+ P AL A A L ++ L + G+DW EW+ + I+ Sbjct: 236 QAMAELGCGPAQQAQAALARAGTALAQLQLAPHDLLVLYQQAGQDWQEWTQTMRIA 291 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 522,316 Number of extensions: 21813 Number of successful extensions: 57 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 2 Length of query: 151 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 74 Effective length of database: 1,255,275 Effective search space: 92890350 Effective search space used: 92890350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)