BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0270.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311810|ref|YP_003775902.1| two component sensor histidine ... 535 e-153 gi|300311887|ref|YP_003775979.1| two component sensor histidine ... 123 4e-29 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 86 1e-17 gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 76 6e-15 gi|300313070|ref|YP_003777162.1| two component sensor histidine ... 75 1e-14 >gi|300311810|ref|YP_003775902.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 535 Score = 535 bits (1378), Expect = e-153, Method: Compositional matrix adjust. Identities = 274/531 (51%), Positives = 367/531 (69%), Gaps = 4/531 (0%) Query: 1 MKLFRKGLLLVAIPGIFELGLLGILYKSQENATQAEVLALHTKNVIIQAGEVREPVLLQS 60 M+L KGLL+VAIPG+FE+ LL L+KSQ +A AE A H+ VI +A +VREP+LL+S Sbjct: 1 MRLLTKGLLIVAIPGLFEIVLLAALFKSQADAADAERWAAHSAEVINKAADVREPLLLES 60 Query: 61 ARLRAAIILNDPAGLEKSDLWDSIEKELGELRELVGDNAEQLRRVAQIGASVQEYRQWTD 120 AR+R AII++DP+ + + +LW +E +LG+L LV DN EQ V + V ++R W D Sbjct: 61 ARIRNAIIIDDPSPISRPELWTDLEIKLGDLARLVSDNPEQSHSVQALRELVGQFRVWAD 120 Query: 121 LQTELLRNDKHDELVAALRDPNARRLIQVIQRQLVEFIKVEEELDQERLSSVVEARRMQN 180 E ++ + DELV LRD + +L F+ E L R ++ AR+ Q Sbjct: 121 SARERMQRGQRDELVRQLRDEQGPHRLSDFLNRLDGFLNEERHLGSLRNQALQSARQTQT 180 Query: 181 IALVIALLGSVLAAALAARIFSHDVGGRLAVLTGNAHKLTEGGVLAQRVGGNDEITELDK 240 L+ A++GS+LAAALA+ F+ +GGR++VL NA +L +G LA RV G+DEI+ LD Sbjct: 181 AWLIAAVIGSILAAALASVAFTRSIGGRISVLIVNARRLADGLPLAARVKGHDEISLLDD 240 Query: 241 VLHESSARLRQAEEDARVYREELERHAHELTSVNDDLQRQTQDNEMFIYSVSHDLRSPLV 300 LH SS RL++A + + YR +LER A ELT +N DL +QTQDNEMFIYSVSHDLRSPLV Sbjct: 241 ALHRSSERLQEASQQSEQYRSQLERRARELTELNADLLQQTQDNEMFIYSVSHDLRSPLV 300 Query: 301 NLQGFSKELHHSVGELHETIDASDLNDKDKQRIRDIIEDDVEVSLRFIRTAVTRSAAIID 360 NLQGFSKE+ H+ +L + A + D DK ++R ++ DD++ +LRFI+ AVTRSA IID Sbjct: 301 NLQGFSKEIGHATTDLLNAVQALPVPDADKAKLRSVVADDIQPALRFIQNAVTRSAGIID 360 Query: 361 AMLRLSRVGRVEYQSQETNVNEVVGRVIDAMSNTIRERQAVV-VSQPLPPCYGDPTAVEQ 419 +MLRLSR GRVEYQ ++N + R++ AMS +IR++ AVV V+ LPP +GDPTA+EQ Sbjct: 361 SMLRLSRAGRVEYQPVMLDMNVIAQRIVAAMSGSIRDKGAVVEVAPELPPAFGDPTAIEQ 420 Query: 420 IFGNLIGNAVNYLNPKRAGTVEIGVLPPDPGQ---PDFRTYYVRDNGLGIPADYLGKMFS 476 + GNL+GNAVNYL+P R G + I L + G P+ Y VRDNGLGIP Y+ KMF Sbjct: 421 VLGNLVGNAVNYLDPARPGRITIDHLREEGGAATGPETNVYVVRDNGLGIPPAYMHKMFI 480 Query: 477 AFHRLHADIAQGEGVGLALIKRIVLRHGGKIWVESTEGAGTTFYTSLPVRP 527 AF RLH + A GEG+GLAL+KR+V RHGG+IWVESTEG G++FY +LP RP Sbjct: 481 AFQRLHGNAAPGEGIGLALVKRVVERHGGRIWVESTEGQGSSFYVALPARP 531 >gi|300311887|ref|YP_003775979.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 633 Score = 123 bits (308), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 89/286 (31%), Positives = 141/286 (49%), Gaps = 37/286 (12%) Query: 241 VLHESSARLRQAEEDARVYREELERHAHELTSVNDDLQRQTQDNEMFIYSVSHDLRSPLV 300 V+++ S R R AEE+ +L+R A L SVN +L E F YSVSHDLR+PL Sbjct: 355 VIYDISDRKR-AEEEIEALNADLKRQALHLHSVNKEL-------ESFSYSVSHDLRAPLR 406 Query: 301 NLQGFSKELHHSVGELHETIDASDLNDKDKQRIRDIIEDDVEVSLRFIRTAVTRSAAIID 360 + G++ L D I++ L+ IR V + +I+ Sbjct: 407 AISGYAMILEEDYA--------------------DAIDEKGREQLQVIRRNVRKMDELIN 446 Query: 361 AMLRLSRVGRVEYQSQETNVNEVVGRVIDAMSNTIRERQAVVVS-QPLPPCYGDPTAVEQ 419 +L+L++ E + +++++V +VI + RE V+ PL + + Q Sbjct: 447 DLLKLAKSTTGELTLERFSMDDLVRQVIAGLR---RENPNVLFDVPPLEGAVANRGLITQ 503 Query: 420 IFGNLIGNAVNYLNPKRAGTVEIGVLPPDPGQPDFRTYYVRDNGLGIPADYLGKMFSAFH 479 ++ NL+ NAV + + V + + +P+ Y V DNG+G Y K+F F Sbjct: 504 VWENLLSNAVKFSSKSEQPAVRVSM----QVEPEEYIYGVHDNGVGFDMRYAHKLFGTFQ 559 Query: 480 RLH-ADIAQGEGVGLALIKRIVLRHGGKIWVESTEGAGTTFYTSLP 524 RLH + G G+GLAL++RIV+RH G++W +S G G +FY SLP Sbjct: 560 RLHRQEEYAGTGIGLALVQRIVIRHTGRVWAQSKPGEGASFYFSLP 605 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 85.5 bits (210), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 76/279 (27%), Positives = 129/279 (46%), Gaps = 37/279 (13%) Query: 257 RVYREELERHAHELTSVNDDLQRQTQDNEMFIYSVSHDLRSPLVNLQGFSKELHHSVGEL 316 R+ RE R A +L + F+ ++SH++R+P+ + G + L Sbjct: 61 RLLREN-ARTAEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLR------ 113 Query: 317 HETIDASDLNDKDKQRIRDIIEDDVEVSLRFIRTAVTRSAAIIDAMLRLSR--VGRVEYQ 374 S D + IED L I++ +L S+ VG+++ Sbjct: 114 ------STRLDTQQAEFAKAIEDSAHALL-----------GIVNDVLDFSKIDVGKMDIA 156 Query: 375 SQETNVNEVVGRVIDAMSNTIRERQAVVVSQ---PLPP-CYGDPTAVEQIFGNLIGNAVN 430 E ++ +VV +A+S RE++ +++ LPP GDP + QI NL+GNA+ Sbjct: 157 VVEFDLAKVVEASAEALSFKAREKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALK 216 Query: 431 YLNPKRAGTVEIGVLPPDPGQPDFRTYY-VRDNGLGIPADYLGKMFSAFHRLHADIAQ-- 487 + + AG V + V R + VRD+G+G+ D L ++FS F + + + + Sbjct: 217 FTH---AGEVLLRVQALQVTSDHCRVRFEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQY 273 Query: 488 -GEGVGLALIKRIVLRHGGKIWVESTEGAGTTFYTSLPV 525 G G+GL++ KR+V GG I VES G G+ F+ L + Sbjct: 274 GGTGLGLSICKRLVELMGGSIGVESALGTGSCFWFELGI 312 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 76.3 bits (186), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 70/277 (25%), Positives = 116/277 (41%), Gaps = 59/277 (21%) Query: 262 ELERHAHELTSVNDDLQRQTQDNEMFIYSVSHDLRSPLVNLQ---------GFSKELHHS 312 E + A + + + ++R +D E S+SHDLR+P+ L+ + H Sbjct: 249 EYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDAMRTDFHDD 308 Query: 313 VGELHETIDASDLNDKDKQRIRDIIEDDVEVSLRFIRTAVTRSAAIIDAMLRLSRVGRVE 372 + EL + + + KD DI E+ EV L A++ M+R +R+ E Sbjct: 309 LDELDMMVKGALQSVKDS----DIHENRTEVRLD----------ALVLRMIRDARLAGHE 354 Query: 373 YQSQETNVNEVVGRVIDAMSNTIRERQAVVVSQPLPPCYGDPTAVEQIFGNLIGNAVNYL 432 + +N V+++PL A+++ GNL NA+ Y Sbjct: 355 IAFTPSGIN--------------------VIAKPL--------ALKRAIGNLFDNALYY- 385 Query: 433 NPKRAGTVEIGVLPPDPGQPDFRTYYVRDNGLGIPADYLGKMFSAFHRLHADIAQ---GE 489 VEI + P + D +RD+G G+P D +G +F + RL AQ G Sbjct: 386 ----GQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGM 441 Query: 490 GVGLALIKRIVLRHGGKIWVESTEGAGTTFYTSLPVR 526 G+GL + + IV HGG++ + + G LP R Sbjct: 442 GLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR 478 >gi|300313070|ref|YP_003777162.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 939 Score = 75.1 bits (183), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 68/253 (26%), Positives = 106/253 (41%), Gaps = 31/253 (12%) Query: 287 FIYSVSHDLRSPLVNLQGFSKELHHSVGELHETIDASDLNDKDKQRIRDIIEDDVEVSLR 346 + ++SHDLR+PL +L G + D N + ++DD R Sbjct: 711 LLSAISHDLRTPLTSLVG--------------SADVLAANAR--------LDDDARAMAR 748 Query: 347 FIRTAVTRSAAIIDAMLRLSRV--GRVEYQSQETNVNEVVGRVIDAMSNTIRERQAVVVS 404 I + +++ +L ++R+ G V Q + E+ G + ++ + RQ Sbjct: 749 TISEQSQKMVGLMNNLLDMARLQAGVVTLNRQWNALEELAGSALRLLAKALEGRQVDTRI 808 Query: 405 QP-LPPCYGDPTAVEQIFGNLIGNAVNYLNPKRAGTVEIGVLPPDPGQPDFRTYYVRDNG 463 P LP D +E++ NL+ NA+ Y P + V D G P + V D G Sbjct: 809 APELPLLRVDAVLLERVLANLVENAIKYSPPGTPIGISAHVEHGD-GAPQVKVC-VMDQG 866 Query: 464 LGIPADYLGKMFSAFHRLHADIAQ-GEGVGLALIKRIVLRHGGKIWVESTE-GAGTTFYT 521 G P F F R + A G G+GLA+ + I+ H G+IW+ E G G T Sbjct: 867 RGFPPTMTQHAFEKFTRGDNESATPGVGLGLAICRAIIEAHQGRIWIAPQEAGQGATVCF 926 Query: 522 SLPVRPIQQVTPP 534 +LPV Q PP Sbjct: 927 TLPVE--TQPAPP 937 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,629,492 Number of extensions: 69411 Number of successful extensions: 466 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 451 Number of HSP's successfully gapped: 25 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)