BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0258.p (578 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I M... 541 e-155 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 439 e-124 gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sen... 436 e-123 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 432 e-122 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 424 e-120 >gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I MCP-I serine chemoreceptor transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 556 Score = 541 bits (1395), Expect = e-155, Method: Compositional matrix adjust. Identities = 307/519 (59%), Positives = 386/519 (74%), Gaps = 6/519 (1%) Query: 2 FKNFSIKARLVFVVSFLSLLLALFGAVGLTSLQQTNSALKSLYEDRVVALGQLAQVTTAV 61 FSIK RL+ +SFL+++L G VGL SL TN ++SLYEDR+VA L Q+ + + Sbjct: 1 MNKFSIKIRLILAISFLTIMLLASGIVGLVSLHSTNEKMRSLYEDRLVAYAYLEQIESTL 60 Query: 62 ESGRYAISTAIVGDS------GEIDKNMDALEKTLQDGEKVWQAYLATSLTAEEKQLTEQ 115 ES R I I DS EID+ +D L L+ E V ++Y AT LT EE L E+ Sbjct: 61 ESARSGIYGIITLDSSEGWDPNEIDRRLDLLVNNLKTVEPVIRSYQATYLTPEESTLLER 120 Query: 116 FTAQKKKFIAEGVKPAVEALHNRDFQGATDLYNGPLLEIFKQINVTMAKLTRLQQDVSKQ 175 Q++ + EGV PAVE+L R FQGA D+Y+G + + + ++ T++ L LQ +V ++ Sbjct: 121 LLKQRQHYADEGVMPAVESLRKRSFQGADDIYSGAMRKQWDAVHQTLSDLKELQINVGRE 180 Query: 176 LYNESQARYDTFLTLTIAAIVIGLSVALIMGIWLVRAISGPLNEAVRVARGVADGDLTQD 235 LY Q RY + + + A+ GL VA++ LVRAIS PL +AV +A+ VA GDL++ Sbjct: 181 LYEHEQQRYRQLILMALLAMGAGLIVAVLEAWLLVRAISQPLTQAVGIAQAVAGGDLSRQ 240 Query: 236 IMVHSRDEAGQLMEALKTMNARLQQIVGEVRVSTDTISTASSEIASGNLDLSARTESQAG 295 I V S+DE GQLM+ALK M RL QIV VR T+TI+ AS EI SGNLDLSARTE QAG Sbjct: 241 IEVRSQDETGQLMQALKDMTQRLYQIVAVVRQGTETITMASKEITSGNLDLSARTEQQAG 300 Query: 296 SLEETASAMEELTSTVKQNADNARQANQLAASASHIAQEGGNVVSQVVVTMNNINDSSKK 355 SLEETASAM +LTSTVKQNADNA QANQLAASAS +A GG VV QVV TM +IN SS + Sbjct: 301 SLEETASAMAQLTSTVKQNADNASQANQLAASASEVAVRGGEVVRQVVDTMTSINQSSTR 360 Query: 356 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKTL 415 IVDII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQR+A+AA+EIK L Sbjct: 361 IVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRAASAAREIKDL 420 Query: 416 IDDSVQKVGEGSKLVEQAGVTMDEVVASVKRVTDVMGEITAASQEQSSGIEEVNRAITQM 475 IDDSV+KVG+GS LV+QAG TM+EVV +V++VTD++GEI++ASQEQS GI+E++RAI M Sbjct: 421 IDDSVEKVGKGSALVDQAGKTMEEVVTAVRKVTDMVGEISSASQEQSQGIQEIDRAIVLM 480 Query: 476 DETTQQNAALVEQAAAAAQSLQDQAQRLTQSVSVFKLDG 514 D +TQQNAALVEQAAAAAQ+L+DQA++L+ ++S F+L G Sbjct: 481 DSSTQQNAALVEQAAAAAQALEDQAEKLSLAMSAFRLGG 519 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 439 bits (1130), Expect = e-124, Method: Compositional matrix adjust. Identities = 284/522 (54%), Positives = 352/522 (67%), Gaps = 22/522 (4%) Query: 2 FKNFSIKARLVFVVSFLSLLLALFGAVGLTSLQQTNSALKSLYEDRVVALGQLAQVTTAV 61 F N I +L+ + + +L + G + L+ N A + + + + + ++ Sbjct: 4 FNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQASL 63 Query: 62 ESGRYA-ISTAIVGDSGEIDKNMDALEKTLQDGEKVWQAYLATSLTAEEKQLTEQFTAQK 120 R + + E + + A++ L K AY A AEEK L E+F+ Sbjct: 64 PRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFSKSF 123 Query: 121 KKFI----------AEGVKPAVEALHNRDFQGATDLYNGPLLEIFKQINVTMAKLTRLQQ 170 ++ AEG + AL F+G ++ ++E +I ++ Sbjct: 124 ATYLEIDKELARISAEGKEEEARAL----FKGDSNKTFRSMIENMDEIIKVNDAGSKRSD 179 Query: 171 DVSKQLYNESQARYDTFLTLTIAAIVIGLSVALIMGIWLVRAISGPLNEAVRVARGVADG 230 D+++Q+Y + ++ +AAIV+ +AL + + + RA+S PLNEAV VA VA G Sbjct: 180 DMAEQVYRSAT----LWIIGLLAAIVV---IALGLAVMVARAVSRPLNEAVAVAERVAQG 232 Query: 231 DLTQDIMVHSRDEAGQLMEALKTMNARLQQIVGEVRVSTDTISTASSEIASGNLDLSART 290 DLT DI S DE G LM +L+ MN LQQIVGEVR TDTI+TAS EIA+GNLDLS+RT Sbjct: 233 DLTMDIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRT 292 Query: 291 ESQAGSLEETASAMEELTSTVKQNADNARQANQLAASASHIAQEGGNVVSQVVVTMNNIN 350 E QAGSLEETASAMEELT+TVKQNADNARQANQLAASAS IA +GG VV QVV TM I Sbjct: 293 EEQAGSLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGIT 352 Query: 351 DSSKKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAK 410 +SS +I DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AAK Sbjct: 353 ESSNRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAK 412 Query: 411 EIKTLIDDSVQKVGEGSKLVEQAGVTMDEVVASVKRVTDVMGEITAASQEQSSGIEEVNR 470 EIK LIDDSV+KVG GS+LVE+AG TM EVVASV+RVTDV+ EI+AAS EQS+GIEE+NR Sbjct: 413 EIKQLIDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINR 472 Query: 471 AITQMDETTQQNAALVEQAAAAAQSLQDQAQRLTQSVSVFKL 512 AITQMDE TQQNAALVE+AAAAAQSLQDQA RL+Q VSVFKL Sbjct: 473 AITQMDEVTQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKL 514 >gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 436 bits (1121), Expect = e-123, Method: Compositional matrix adjust. Identities = 287/590 (48%), Positives = 359/590 (60%), Gaps = 39/590 (6%) Query: 2 FKNFSIKARLVFVVSFLSLLLALFGAVGLTSLQQTNSALKSL----------YEDRVVAL 51 F N I +L+ + + LL G + L + N A L D VAL Sbjct: 4 FYNRRIATKLLLAFACVLLLTGGLGIFSIVQLHKVNQAATELATNWLPSIRSLNDMKVAL 63 Query: 52 GQLA--QVTTAVESGRYAISTAIVGDSGEIDKNMDALEKTLQDGEKVWQAYLATSLTAEE 109 ++ Q A+ G S+A++G ++KN++AL + + Y E Sbjct: 64 SRVRANQAQLALYDGDEKQSSAVIG---LMNKNLEALAEARKQ-------YTGQISEPGE 113 Query: 110 KQLTEQFTAQKKKFIA-EGVKPAVEALHNRDFQGATDLYNGPLLEIFKQINVTMAKLTRL 168 K L A+ KFIA G + A+ A L GP E + + + KL ++ Sbjct: 114 KALFPGVNARIDKFIALHG--DTLAAVRAGKLDEAKRLLLGPAFENYLSLLNDLDKLAQV 171 Query: 169 QQDVSKQLYNESQARYDTFLTLTIAAIVIGLSVALIMGIWLVRAISGPLNEAVRVARGVA 228 D S Q ++ YD +A ++ L L + +++ R I+ PL A A +A Sbjct: 172 NLDGSVQSTRSAETTYDQAQVWVVAILIGALLAGLALALFIARVIATPLRSAGEFAHLIA 231 Query: 229 DGDLTQDIMVHSRDEAGQLMEALKTMNARLQQIVGEVRVSTDTISTASSEIASGNLDLSA 288 GDLT DI S DE GQL AL MN L++IV +VR TD I+TAS +I+SGNLDLS+ Sbjct: 232 AGDLTADITARSDDETGQLTAALAAMNTSLRRIVSQVRSGTDAIATASGQISSGNLDLSS 291 Query: 289 RTESQAGSLEETASAMEELTSTVKQNADNARQANQLAASASHIAQEGGNVVSQVVVTMNN 348 RTE QA SLEETASAMEELTSTV+QNA+NARQANQLA SAS IA+EGG VVSQV+ TM + Sbjct: 292 RTEEQASSLEETASAMEELTSTVRQNAENARQANQLALSASTIAEEGGKVVSQVIETMGS 351 Query: 349 INDSSKKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAA 408 I+ SSKKIVDIISVID IAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA A Sbjct: 352 IDASSKKIVDIISVIDSIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSATA 411 Query: 409 AKEIKTLIDDSVQKVGEGSKLVEQAGVTMDEVVASVKRVTDVMGEITAASQEQSSGIEEV 468 AKEIKTLID+SVQ+VG GS LV++AGVT+ EVV+SV+RVTDVM EI+AAS EQ+ GIE+V Sbjct: 412 AKEIKTLIDNSVQQVGLGSGLVQKAGVTISEVVSSVQRVTDVMSEISAASGEQTQGIEQV 471 Query: 469 NRAITQMDETTQQNAALVEQAAAAAQSLQDQAQRLTQSVSVFKLDGNRQLTSTPKTSTPV 528 N+AITQMDE TQQNAALVE+AA+A+QSL QAQRL++ V VF+L G + Sbjct: 472 NQAITQMDEVTQQNAALVEEAASASQSLSHQAQRLSELVGVFRLPGAARQQVPEVAVVAT 531 Query: 529 RTVDITPRPVAVTAEKKPAATVARTAKPAVAPALPASAAVKPNDDDWEQF 578 TPR AV A + A PA A + D+ WE F Sbjct: 532 AAPAYTPRRPAVAG--------------ATSTATPALAVAQGPDEQWETF 567 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 432 bits (1112), Expect = e-122, Method: Compositional matrix adjust. Identities = 297/598 (49%), Positives = 375/598 (62%), Gaps = 42/598 (7%) Query: 2 FKNFSIKARLVFVVSFLSLLLALFGAVGLTSLQQTNSALKSLYEDRVVALGQLAQVTTAV 61 FKN + RL V +F+ LLA+ + N A+ + DR + + + V Sbjct: 5 FKNLKVAVRLAAVFAFIVALLAVVSVTAFIKINNINRAIDQIVNDRYLKVRWAFDLRDGV 64 Query: 62 -ESGRYAISTAI-VGDSGEIDKNMDALEKTLQDGEKVWQAYLATSLTA----EEKQLTEQ 115 E +Y AI + + +K LE ++ ++ A +TA + K L E Sbjct: 65 NEQIKYLRGIAIDIENRPANEKRFAQLEVAVKQTKEALDKITARQVTAVGQKKAKALDEA 124 Query: 116 ---FTAQKKKFIAEGVKPAVEALHNRDFQGATDLYNG--PLLEIFKQINVTMAKLTRLQQ 170 F A K F+A + + TD N L F ++ +LQ Sbjct: 125 RIAFDASKADFLALVKAGNSTSADEYVLRKITDTQNNYLALARAFAD-----SQDAQLQA 179 Query: 171 DVSKQLYNESQARYDTFLTLTIAAIVIGLSVALIMGIWLVRAISGPLNEAVRVARGVADG 230 + +K + + + A + +T+A + + A+I+G + R+I PL EAVRVA VA G Sbjct: 180 EGAKAVADGAMA-----IEVTLACSMAAVLAAIILGYLMARSIVKPLTEAVRVAENVAAG 234 Query: 231 DLTQDIMVHSRDEAGQLMEALKTMNARLQQIVGEVRVSTDTISTASSEIASGNLDLSART 290 DLT I HSRDE GQLM+AL+ MN L +IV VR T++I+TAS EIASGNLDLSART Sbjct: 235 DLTTRIEPHSRDETGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSART 294 Query: 291 ESQAGSLEETASAMEELTSTVKQNADNARQANQLAASASHIAQEGGNVVSQVVVTMNNIN 350 E QAGSLEETASAMEE+TSTV+QNADNARQANQ+A SAS IA +GG +V +VV TM+ IN Sbjct: 295 EQQAGSLEETASAMEEMTSTVRQNADNARQANQMAVSASEIALQGGQIVGRVVHTMDEIN 354 Query: 351 DSSKKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAK 410 SS+KIVDII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSAAAAK Sbjct: 355 QSSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAK 414 Query: 411 EIKTLIDDSVQKVGEGSKLVEQAGVTMDEVVASVKRVTDVMGEITAASQEQSSGIEEVNR 470 EIK LI DSV KV GS LV QAG TM++VV+SV+ VTD++ EI+AAS EQSSGIEE+NR Sbjct: 415 EIKALIADSVGKVEGGSALVAQAGATMEQVVSSVRSVTDIVAEISAASAEQSSGIEEINR 474 Query: 471 AITQMDETTQQNAALVEQAAAAAQSLQDQAQRLTQSVSVFKLDGNRQLTSTPKTSTPVRT 530 A+TQMDE TQQNAALVEQAAAAA+S+Q+QA RLTQS+S+FKL+ + S P + PVRT Sbjct: 475 AVTQMDEGTQQNAALVEQAAAAAKSMQEQAVRLTQSISIFKLE--QGAASIP--AAPVRT 530 Query: 531 VDITPRPVAVTAEKKPAATVARTAKPAVAP----------ALPASAAVKPNDDDWEQF 578 A P A+ A TA+P V+P A A + ++D WE F Sbjct: 531 -------ALAPAAPAPTASRAPTARPRVSPAITAAPTVAAAPALKAPARVDEDAWETF 581 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 424 bits (1090), Expect = e-120, Method: Compositional matrix adjust. Identities = 303/612 (49%), Positives = 383/612 (62%), Gaps = 71/612 (11%) Query: 2 FKNFSIKARLVFVVSFLSLLLALFGAVGLTSLQQTNSALKSLYEDRVVALGQLAQVTTAV 61 F + I +L+ + ++A G + L Q NSA + + + ++ LA++ A+ Sbjct: 4 FYDLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQLAM 63 Query: 62 ESGR-YAISTAIVGDSGEIDKNMDALEKTLQ---DGEKVWQAYLATSLT-AEEK------ 110 R + + + D+ E ++ +E+ +Q + K QA T ++ EEK Sbjct: 64 SRLRSFELQHILAKDAKEYEE----VERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEV 119 Query: 111 -QLTEQFTAQKKKFIAEGVKPAVEALHNRDFQGATDLYNGPLLEIFK--QINVTMAKLTR 167 +L F ++ K IA + E N +T Y L +I K +N A ++ Sbjct: 120 EKLIATFVSEHDKIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAVNDKGADISN 179 Query: 168 LQQDVSKQLYNESQARYDTFLTLTIAAIVIGLSVALIMGIWLVRAISGPLNEAVRVARGV 227 Q D +Y +QAR ++ +T+AA IGL A+++ +W+ R I+ PL EAV VA+ V Sbjct: 180 KQADA---IY--AQARL--WIIVTVAA-CIGL--AMLLAVWVSRLIARPLEEAVSVAQRV 229 Query: 228 ADGDLTQDIMVHSRDEAGQLMEALKTMNARLQQIVGEVRVSTDTISTASSEIASGNLDLS 287 A GDLT I +DE GQL+ ALK+MN L IVG+VR+ T TI+TAS+EIASGNLDLS Sbjct: 230 AGGDLTTRIEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLS 289 Query: 288 ARTESQAGSLEETASAMEELTSTVKQNADNARQANQLAASASHIAQEGGNVVSQVVVTMN 347 +RTE QA SLEETASAMEELTSTVKQNADNARQANQLA SAS +A +GG VV+QVV TM Sbjct: 290 SRTEEQASSLEETASAMEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTMG 349 Query: 348 NINDSSKKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAA 407 +IN SS+KI DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA Sbjct: 350 SINASSRKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAG 409 Query: 408 AAKEIKTLIDDSVQKVGEGSKLVEQAGVTMDEVVASVKRVTDVMGEITAASQEQSSGIEE 467 AAKEIK LI+ SV +V GSKLVE+AG+TM EVV SV+RVTD++GEI++ASQEQS GIE+ Sbjct: 410 AAKEIKELIEGSVAQVDMGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEGIEQ 469 Query: 468 VNRAITQMDETTQQNAALVEQAAAAAQSLQDQAQRLTQSVSVFKLDGNRQLTSTPKTSTP 527 VN AI QMDE TQQNAALVE+AAAAAQSLQ+QA+ LTQ+V FKL+ Q P + P Sbjct: 470 VNLAIAQMDEVTQQNAALVEEAAAAAQSLQEQAETLTQTVGFFKLE---QQPDEPASRPP 526 Query: 528 VRTVDITPRPVAVTAEKKPAATVARTAKPAVAPA---------------------LPASA 566 VR AA+VARTA APA LP A Sbjct: 527 VR-----------------AASVARTAAVRHAPATRPAATLAASAAPAKPASGSRLPGGA 569 Query: 567 AVKPNDDDWEQF 578 A + WEQF Sbjct: 570 A--EDGGSWEQF 579 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.124 0.322 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,206,308 Number of extensions: 40671 Number of successful extensions: 574 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 336 Number of HSP's successfully gapped: 72 Length of query: 578 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 488 Effective length of database: 1,193,720 Effective search space: 582535360 Effective search space used: 582535360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)