BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0257.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314041|ref|YP_003778133.1| transporter permease [Herbaspir... 64 1e-11 >gi|300314041|ref|YP_003778133.1| transporter permease [Herbaspirillum seropedicae SmR1] Length = 311 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 83/301 (27%), Positives = 138/301 (45%), Gaps = 29/301 (9%) Query: 7 ITGIILPVFAIIAIGYGYARWRGEVVRSDMASVNRVSMEVLAPLMVF---SALSAKDF-D 62 I I+ PVF++I +G+ R R + +NR + + P ++F S LSA DF + Sbjct: 4 IFNIVFPVFSLIFLGWA-CRKRNILSGGAATELNRFVIWLALPALLFDSMSRLSAADFAN 62 Query: 63 LAHNGWLILASVIISLGSALLAW---PVATMLGYDKRTFLPPMMYNNGGNMGVPLMLLAF 119 L L++ ++ L +A L W A + D Y N G +G+PL LAF Sbjct: 63 LRFIAVFALSTALVFLLTAWLRWRERAAAADIIIDSLG----SAYANVGFIGIPLCYLAF 118 Query: 120 GPESLSAAVALFTAATFVYFSLGIRMLQ---HGRA----ITTRPGSFLKLFKSPIMVAMV 172 G + L +V + F++ I +L+ H +A + G L ++PIM+A V Sbjct: 119 GHDGLPPSVIAMVMTACLLFAVAIVLLETCLHAQAGLAHTVAKVGR--SLLRNPIMIAPV 176 Query: 173 LGVLFAVLHVPLPPPVFSAMKLLGEASIPIMLFALGVRMMD--ISFKSWHIGLVGAVTC- 229 G++ AV +P+P V K+LG A+ P L +LG+ + + + L+ A Sbjct: 177 AGIIVAVFGLPVPSGVQQLFKILGGAAGPCALVSLGLFLAQPVQDRQGASVSLLVAFKLL 236 Query: 230 --PLVGLAVAFALDQALPLTAGQRGQMYLFGALPPAVLCFMVAEQYRQEPDKVASIVLMG 287 P + +A+ L LPL L A+P FM+AE Y++E ++ +L+ Sbjct: 237 LQPAITAVLAYWL---LPLPRLWADAAVLLSAMPIGTGPFMLAELYQREVTAMSRAILVS 293 Query: 288 N 288 Sbjct: 294 T 294 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 958,506 Number of extensions: 38931 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 2 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)