BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0256.p (639 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 992 0.0 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 244 1e-65 gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 226 3e-60 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 150 2e-37 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 992 bits (2564), Expect = 0.0, Method: Compositional matrix adjust. Identities = 531/640 (82%), Positives = 563/640 (87%), Gaps = 1/640 (0%) Query: 1 MMHLPPMLGYLVVGIVIGPHGLGFADDNETTHALAEFGVVFLMFSIGLEFSLPKLSAMRH 60 MM LPPMLGYLVVGI+IGPHGLGFA+DNETTHAL EFGVVFLMFSIGLEFSL KLSAMRH Sbjct: 24 MMQLPPMLGYLVVGIIIGPHGLGFAEDNETTHALGEFGVVFLMFSIGLEFSLAKLSAMRH 83 Query: 61 IVFGLGVAQVLATIAVAMVFGWSAAYILPQLNEISWPAAFALGGALAMSSTAIVSKLLTE 120 IVFGLGVAQV TIA MV GW AA LPQ ISW AAFALGGAL MSSTAIVSKLLTE Sbjct: 84 IVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQAAFALGGALTMSSTAIVSKLLTE 143 Query: 121 RLELETEHGRRILGILLFQDLALVPLLIIVPALAKNSGNLVVTLAWASIKALVVLALLLF 180 RLELETEHGRRILG+LLFQDLALVPLLI+VPALA SGN++VTL WA+ KALVVLALLLF Sbjct: 144 RLELETEHGRRILGVLLFQDLALVPLLIVVPALANESGNIMVTLGWAAGKALVVLALLLF 203 Query: 181 FGQKLMRSWFQIVVKRRSQELFMLNLLMITLGAAWITEKAGLSLALGAFIAGMLISETEY 240 FG KLMR WF+IVVKRRSQELFMLNLL+ITLGAAWITEKAGLSLALGAFIAGMLISETE+ Sbjct: 204 FGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWITEKAGLSLALGAFIAGMLISETEF 263 Query: 241 KHQVEEDIKSFRDVLLGLFFITIGMLLNVHLVLEHWWLVLLLLVGPVLLKFVLIAGLAKL 300 KHQVEEDIKSFRDVLLGLFFITIGMLLNV V++HW+LVLLLL GPVLLKF LIA LA+L Sbjct: 264 KHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHWFLVLLLLTGPVLLKFGLIAALARL 323 Query: 301 FGSTTGVALRTGLALAQAGEFGFVLLNQAGGLQLMDPLLIQVILASMVLSMLAAPFLLAK 360 FGST GVALR GL LAQAGEFGFVLL+QAGGL+L+DP+LIQVILA+MVLSMLA PF+LAK Sbjct: 324 FGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDPMLIQVILAAMVLSMLATPFILAK 383 Query: 361 SDAIVMKLSANEWMLQSLALTQLATRTMSSQKHVIIAGFGRSGQSLAKLLEEEGIPYHAL 420 SDAIVMK SANEWMLQSLALTQLATRTM QKHVIIAGFGRSGQSLA+LLEEEGI YHAL Sbjct: 384 SDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVIIAGFGRSGQSLARLLEEEGIAYHAL 443 Query: 421 EMDPDLVHDTREAGANVSYGDAARRESLVAAGIHRAAALVVTYASTPSALKVLHHANELA 480 EMDP+LV D R GANVSYGDAARRESLVAAGIHRAAALVVTYASTPSALKVLHHA EL Sbjct: 444 EMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAAALVVTYASTPSALKVLHHAAELE 503 Query: 481 PTLPVIVRSHDDADLDKLLEAGATEVVPEALEGSLMLASHALVMLGVPLRRVVHRVQSAR 540 P LPVIVRSHDDADL+KLL AGATEVVPEALEGSLMLASHALVMLGVPLRRVVHRVQ AR Sbjct: 504 PELPVIVRSHDDADLEKLLAAGATEVVPEALEGSLMLASHALVMLGVPLRRVVHRVQDAR 563 Query: 541 DERYTSLRGYFHGASDVPEDNDNLNVRLQSVALNEGASAIGKTLYQVALE-QFDTEVTML 599 DERY SLRGYFHG SD D +NVRLQ+V L GASA+G TL ++ L EV L Sbjct: 564 DERYASLRGYFHGMSDGEADGVGMNVRLQAVTLIGGASALGHTLAEIDLRAHCGAEVASL 623 Query: 600 RRGKLRVGFTPDTVLQTGDIVVLRGTGENILRAEKRLLKK 639 RR K R+ TVL GDIVVLRG+ E++LRAEKRLL K Sbjct: 624 RRNKQRIEPPEQTVLMAGDIVVLRGSSESLLRAEKRLLSK 663 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 244 bits (624), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 179/522 (34%), Positives = 275/522 (52%), Gaps = 37/522 (7%) Query: 2 MHLPPMLGYLVVGIVIGPHGLGFADDNETTHALAEFGVVFLMFSIGLEFSLPKLSAMRHI 61 + +PP++GYLV GI IGP GF D + LAE GV+ LMF +GL FSL L ++++I Sbjct: 28 LKMPPLVGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLSVKNI 87 Query: 62 VFGLGVAQVLATIAVAMVFGWSAAYILPQLNEISWPAA--FALGGALAMSSTAIVSKLLT 119 VAQ+ + M GW + WP F G AL+++ST ++ K L Sbjct: 88 AIPGAVAQITIATLLGMALGWL----------LGWPVGEGFLFGLALSVASTVVLLKALQ 137 Query: 120 ERLELETEHGRRILGILLFQDLALVPLLIIVPALAKN--------SGNLVV-TLAWASIK 170 ER +ET+ GR +G L+ +D+A+V L+++PAL+ SGN V+ TL K Sbjct: 138 ERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGNAVLLTLGITLGK 197 Query: 171 ALVVLALLLFFGQKLMRSWFQIVVKRRSQELFMLNLLMITLGAAWITEKA-GLSLALGAF 229 + A++L G++++ + + S+ELF L +L I LG A + G+S ALGAF Sbjct: 198 VVAFAAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSFALGAF 257 Query: 230 IAGMLISETEYKHQVEEDIKSFRDVLLGLFFITIGMLLNVHLVLEHWWL----VLLLLVG 285 AGM++SE+E H+ E+ RD LFF+++GML + +V L VL+++VG Sbjct: 258 FAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVAREPLLVLATVLIIVVG 317 Query: 286 PVLLKFVLIAGLAKLFGSTTGVALRTGLALAQAGEFGFVLLNQAGGLQLMDPLLIQVILA 345 L +++ + FG G AL +LAQ GEF F+L L L+ P+ +IL Sbjct: 318 KSLGALLIV----RAFGYPNGTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILG 373 Query: 346 SMVLSMLAAPFLLAKSDAIVMKLSANEWMLQSLALTQLATRTMSSQKHVIIAGFGRSGQS 405 +LS+L P L + D + A + + + Q HV++ GFGR G+ Sbjct: 374 GAILSILLNPLLFSLLD----RYEARQ---PKAPQGGDEPQKVELQDHVVLVGFGRVGRG 426 Query: 406 LAKLLEEEGIPYHALEMDPDLVHDTREAGANVSYGDAARRESLVAAGIHRAAALVVTYAS 465 + + L +G P+ +E + + R AG YG+AA+ E L AA I RA L+V Sbjct: 427 IGEQLRAQGKPFVVIEAQLENLEALRAAGVPALYGNAAQSELLQAAAIDRARWLLVAIPE 486 Query: 466 TPSALKVLHHANELAPTLPVIVRSHDDADLDKLLEAGATEVV 507 A +V+ +A EL P L V+ R+H DA+++ L + GA V+ Sbjct: 487 VFEAGQVIENALELNPGLQVVARAHSDAEIEHLEKHGAQRVI 528 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 226 bits (577), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 177/527 (33%), Positives = 271/527 (51%), Gaps = 31/527 (5%) Query: 7 MLGYLVVGIVIGPHGLGFADDNETTHALAEFGVVFLMFSIGLEFSLPKLSAMRHIVFGLG 66 +LGYL+ GIVIGP GLG + + +EFGVV L+F IGLE +L A+R +FG G Sbjct: 30 VLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVLLLFLIGLELDPKRLWALRRSIFGWG 89 Query: 67 VAQVLATIAVAMVFGWSAAYILPQLNEISWPAAFALGGALAMS--STAIVSKLLTERLEL 124 QV A + S P L GAL MS STAI L ER Sbjct: 90 SIQVAAVSLALLA----------AALAASVPWRIGLIGALGMSLSSTAIALATLGERKLT 139 Query: 125 ETEHGRRILGILLFQDLALVPLLIIVP----ALAKNSGNLVVTLAW-ASIKALVVLALLL 179 T G ILLFQD+A +P++ VP A ++ SG W + + +V+ALL+ Sbjct: 140 ATPAGSAAFSILLFQDIAAIPMMAAVPLLGVAASQGSGQ-----GWLHAGQVALVIALLI 194 Query: 180 FFGQKLMRSWFQIVVKRRSQELFMLNLLMITLGAAWITEKAGLSLALGAFIAGMLISETE 239 F G+ LMR +I+ K +E+F L++ + + + G+S+ALG F+AG+L++++E Sbjct: 195 FGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMALGTFLAGVLLADSE 254 Query: 240 YKHQVEEDIKSFRDVLLGLFFITIGMLLNVHLVLEHWWLVLLLLVGPVLLKFVLIAGLAK 299 Y+H +E D++ F+ +LLGLFFI +GM ++ + W +L +++G + +K ++ L + Sbjct: 255 YRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLGFLAIKTCVMWALGR 314 Query: 300 LFGSTTGVALRTGLALAQAGEFGFVLLNQAGGLQLMDPLLIQVILASMVLSMLAAPFLLA 359 FG G L L+Q GEF F++ A +++ P +++ + LSML P LL Sbjct: 315 CFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVVVVALSMLVTPLLLL 374 Query: 360 KSDAIVMKLSANEWMLQSLALTQLATRTMSSQKH-VIIAGFGRSGQSLAKLLEEEGIPYH 418 D ++++ WM + + Q H VIIAGFGR GQ + +LL GI Sbjct: 375 VHDRLLVR-----WMEGG---KRRPEDKIDPQDHAVIIAGFGRFGQIIGRLLAANGIGAT 426 Query: 419 ALEMDPDLVHDTREAGANVSYGDAARRESLVAAGIHRAAALVVTYASTPSALKVLHHANE 478 L+ DPD + R+ G V YGDA R + L AG +A LV+ +AL+++ E Sbjct: 427 VLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEMVDKVRE 486 Query: 479 LAPTLPVIVRSHDDADLDKLLEAGATEVVPEALEGSLMLASHALVML 525 P L + R+ + L++ G T + E E +L + L L Sbjct: 487 EFPHLQIAARARNVTHYYDLMDRGVTVLERETFESALHMGRQVLQQL 533 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 150 bits (380), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 106/371 (28%), Positives = 195/371 (52%), Gaps = 22/371 (5%) Query: 5 PPMLGYLVVGIVIGPHGLGF--ADDNETTHALAEFGVVFLMFSIGLEFSLPKLSAMRHIV 62 P +LGY+V G++IGPH + D +T H L+E GV+FL+FS+GLEFSL KL+ + Sbjct: 31 PVVLGYIVAGVIIGPHTPPYDLIQDEKTVHILSELGVIFLLFSLGLEFSLKKLAKVGATA 90 Query: 63 FGLGVAQVLATI----AVAMVFGWSAAYILPQLNEISWPAAFALGGALAMSSTAIVSKLL 118 F A++L I + + FGW A LG LA+SST I+ K L Sbjct: 91 FIGAAAEILLMIWIGYEIGLYFGWKRM------------DAIFLGAMLAVSSTTIIVKAL 138 Query: 119 TERLELETEHGRRILGILLFQDLALVPLLIIVPALAKNSGNLVVTLAWASIKALVV-LAL 177 E + + I GIL+ +D+ + ++ ++ +A SG++ T + ++ LV+ + + Sbjct: 139 NELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVA-TSGSVDSTEVFTTVGKLVLFMTV 197 Query: 178 LLFFGQKLMRSWFQIVVKRRSQELFMLNLLMITLGAAWITEKAGLSLALGAFIAGMLISE 237 L G + + + +S E+ ++ +L + G + K S+ALGAF+ G +++E Sbjct: 198 SLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLVMKLQYSVALGAFLVGAVMAE 257 Query: 238 TEYKHQVEEDIKSFRDVLLGLFFITIGMLLNVHLVLEHWWLVLLLLVGPVLLKFVLIAGL 297 + + H++E ++ RD+ +FF+ IG+L + ++++++W + ++ + V K + GL Sbjct: 258 SRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNVLVKYWMPITVITLAVVFGKLI-SCGL 316 Query: 298 AKLFGSTTG-VALRTGLALAQAGEFGFVLLNQAGGLQLMDPLLIQVILASMVLSMLAAPF 356 G +R G+ LAQ GEF F++ L++ L +++A ++ L P+ Sbjct: 317 GTFIAGQGGRTPMRVGMGLAQIGEFSFIIAALGQSLKVTSDFLYPIVVAVSAVTALLTPY 376 Query: 357 LLAKSDAIVMK 367 L+ +D + K Sbjct: 377 LIKAADPLSAK 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,783,126 Number of extensions: 70416 Number of successful extensions: 380 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 369 Number of HSP's successfully gapped: 5 Length of query: 639 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 548 Effective length of database: 1,188,985 Effective search space: 651563780 Effective search space used: 651563780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 134 (56.2 bits)