BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0245.p (198 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313264|ref|YP_003777356.1| short-chain alcohol dehydrogena... 315 1e-87 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 67 1e-12 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 64 1e-11 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 57 1e-09 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 55 3e-09 >gi|300313264|ref|YP_003777356.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 315 bits (808), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 169/251 (67%), Positives = 182/251 (72%), Gaps = 56/251 (22%) Query: 3 APKKIALVTGAGSGIGKQISLALLRDGYAVVLAGRRQQPLEETVRAAGEFGANALVVPAD 62 APK+IALVTGAGSGIG+QI+LALLR+GY V LAGRRQ LEETVR AGE G NALVV AD Sbjct: 6 APKRIALVTGAGSGIGRQITLALLREGYGVALAGRRQDALEETVRLAGEDGPNALVVAAD 65 Query: 63 VSDPASVKNLFAKTREKFGRLDLLFNNA--------------------------GIF--- 93 V+DPASVK LFAKTRE+FGRLD+LFNNA G+F Sbjct: 66 VTDPASVKQLFAKTRERFGRLDMLFNNAGIFAPPVSLEELSVEQWKAAVDINLTGVFLCS 125 Query: 94 --------------------------APXRPFSVSYTATKHAITGLTKSTSLDGRRYDIA 127 AP RPFS SYTATKHAITGLTK+TSLDGR YDIA Sbjct: 126 QEAFRIMKDQSPRGGRIINNGSISAHAP-RPFSASYTATKHAITGLTKATSLDGRAYDIA 184 Query: 128 CGQIDIGNASTDMTELMKKGTLQADGSTQVEPTMDAGHVAKAILYMDSLPLDANVQFMTV 187 CGQIDIGNASTDMT LMKKGTLQADGST+VEPTMDA HVA+AILYMDSLPL+ANVQFMTV Sbjct: 185 CGQIDIGNASTDMTTLMKKGTLQADGSTKVEPTMDAEHVARAILYMDSLPLEANVQFMTV 244 Query: 188 MATKMPFIGRG 198 MATKMP+IGRG Sbjct: 245 MATKMPYIGRG 255 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 66.6 bits (161), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 36/95 (37%), Positives = 52/95 (54%) Query: 6 KIALVTGAGSGIGKQISLALLRDGYAVVLAGRRQQPLEETVRAAGEFGANALVVPADVSD 65 K+AL+TGA +GIG+ +L R G A++L RRQ LE+ G A VV DV + Sbjct: 7 KVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAGDVGE 66 Query: 66 PASVKNLFAKTREKFGRLDLLFNNAGIFAPXRPFS 100 A+ + A ++FG LD+ NNAG +P + Sbjct: 67 AATHERGVAMALDEFGGLDIAINNAGAVGACKPLA 101 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 63.5 bits (153), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 37/95 (38%), Positives = 53/95 (55%), Gaps = 1/95 (1%) Query: 6 KIALVTGAGSGIGKQISLALLRDGYAVVLAGRRQQPLEETVRAAGEFGANALVVPADVSD 65 K+ ++TGA SG+G+ + L G +VL RR + LE+ V G A+ V DV+ Sbjct: 8 KVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVTTDVAR 67 Query: 66 PASVKNLFAKTREKFGRLDLLFNNAGIFAPXRPFS 100 V+ L AK + FGR+D+L NNAGI P P + Sbjct: 68 RDDVEALVAKGEQHFGRIDVLVNNAGIM-PLAPMA 101 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 56.6 bits (135), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 33/86 (38%), Positives = 47/86 (54%), Gaps = 3/86 (3%) Query: 6 KIALVTGAGSGIGKQISLALLRDGYAVVLAGRRQQPLEETVRAAGEFGANALVVPADVSD 65 K+ +VTG GIG + +G V ++GRR LE+ VR E G A+ VP D + Sbjct: 10 KVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVR---EIGNGAVGVPGDAAK 66 Query: 66 PASVKNLFAKTREKFGRLDLLFNNAG 91 + L+A+ + GRLD+LF NAG Sbjct: 67 LDDLDRLYARVQADHGRLDVLFANAG 92 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 55.5 bits (132), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 31/87 (35%), Positives = 48/87 (55%) Query: 6 KIALVTGAGSGIGKQISLALLRDGYAVVLAGRRQQPLEETVRAAGEFGANALVVPADVSD 65 K+AL+TG+ SGIGK+I++ R G VV+A + + T + G A+ V +V+D Sbjct: 6 KVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVAMNVTD 65 Query: 66 PASVKNLFAKTREKFGRLDLLFNNAGI 92 V A +G LD++ +NAGI Sbjct: 66 ETQVDKGVADAVNTYGGLDIMISNAGI 92 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 598,185 Number of extensions: 24377 Number of successful extensions: 184 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 14 Length of query: 198 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 118 Effective length of database: 1,241,070 Effective search space: 146446260 Effective search space used: 146446260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)