BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0231.p (248 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 122 2e-29 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 93 2e-20 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 93 2e-20 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 88 6e-19 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 86 4e-18 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 122 bits (307), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 86/258 (33%), Positives = 125/258 (48%), Gaps = 14/258 (5%) Query: 5 VIVERSDAIACVTMNQPGRRNALAPEMREALTETLERLACDVDCRAIILAGTDRSFCAG- 63 +++E D IA +T+N+P + N+ M L + + L D R ++L G R FCAG Sbjct: 6 ILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAGQ 65 Query: 64 --GDLSSLPAGDALASRKRMTLAH----RLLRTI------XVDGTAFGAGMGLALACDYV 111 GD + P D + + + + + LR + V+G A GAG L LACD V Sbjct: 66 DLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIV 125 Query: 112 LCSERARFCTAYSNVGLMADMGLLWSLPQRVSAGQVRRLLYTSAVVDGEEALRLGLADQL 171 L + A F + +GL+ D G + LP+ V + + + E+A R GL + Sbjct: 126 LAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKC 185 Query: 172 VHSDELQQAALDLAAQFAAAPPLAVGLTKAALARLPG-SLDTVMGLELDGQLQLFSSADF 230 V D+L + A +AA FA AP + TKA L P +L + LE D L S D+ Sbjct: 186 VDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSRDY 245 Query: 231 TEGRHAFLEKRRPYFQGR 248 EG AFLEKR P F G+ Sbjct: 246 REGVTAFLEKRAPQFTGQ 263 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 93.2 bits (230), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 77/258 (29%), Positives = 124/258 (48%), Gaps = 22/258 (8%) Query: 7 VERSDAIACVTMNQP----GRRNALAPEMREALTETLERLACDVDCRAIILAGT-DRSFC 61 +E++ A +T++ P R ALA LT ++ L D D +++ G ++ F Sbjct: 9 LEKTGHTAVLTLSNPPANTWTRAALAD-----LTRLVKELNADRDIYTLVVTGEGEKFFS 63 Query: 62 AGGDLSSLPAGDALASRK---------RMTLAHRLLRTIXVDGTAFGAGMGLALACDYVL 112 AG DL GD +R+ A R + ++G A G G+ ALACD + Sbjct: 64 AGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRI 123 Query: 113 CSERARFCTAYSNVGLMADMGLLWSLPQRVSAGQVRRLLYTSAVVDGEEALRLGLADQLV 172 E+A+ + VGL+ G +LP V G +R++ V+ E ALR+GL +++V Sbjct: 124 AEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVV 183 Query: 173 HSDELQQAALDLAAQFAAAPPLAVGLTKAAL--ARLPGSLDTVMGLELDGQLQLFSSADF 230 + ++ AL LA Q P +V +K + AR L T++ E + + LF + D Sbjct: 184 PRGQARERALALAQQAEKQSPSSVAASKQLIQGAR-HNPLGTILQTERELFVDLFDTQDQ 242 Query: 231 TEGRHAFLEKRRPYFQGR 248 EG AFLEKR P ++ + Sbjct: 243 REGVQAFLEKRSPQWKNQ 260 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 92.8 bits (229), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 73/251 (29%), Positives = 121/251 (48%), Gaps = 9/251 (3%) Query: 5 VIVERSDAIACVTMNQPGRRNALAPEMREALTETLERLACDVDCRAIILAGTDRSFCAGG 64 + VE + + + +++P NAL+ + L E L + II+ G++++F AG Sbjct: 6 IQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAGA 65 Query: 65 DLSSLPAGDALASRKR--MTLAHRLLRTI------XVDGTAFGAGMGLALACDYVLCSER 116 D+S++ D + K +T LR I V G A G G LA+ CD+++ ++ Sbjct: 66 DISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIAADN 125 Query: 117 ARFCTAYSNVGLMADMGLLWSLPQRVSAGQVRRLLYTSAVVDGEEALRLGLADQLVHSDE 176 A+F +G++ G LP+ VS + L T ++D EEA R GL ++V +D+ Sbjct: 126 AKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIVAADK 185 Query: 177 LQQAALDLAAQFAAAPPLAVGLTKAALAR-LPGSLDTVMGLELDGQLQLFSSADFTEGRH 235 L + A+ A A P + K ++ R +L M E F++ D EG Sbjct: 186 LLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQKEGMK 245 Query: 236 AFLEKRRPYFQ 246 AFLEKR+P F+ Sbjct: 246 AFLEKRQPVFK 256 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 88.2 bits (217), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 80/263 (30%), Positives = 119/263 (45%), Gaps = 34/263 (12%) Query: 6 IVERSD--AIACVTMNQPGRRNALAPEMREALTETLERLACDVDCRAIILAGTDRSFCAG 63 I+ER D I +T+N+P NAL+ EM AL L+ + D R +ILA ++FCAG Sbjct: 8 ILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFCAG 67 Query: 64 GDLSSLPAGDALASRKRMTLAHR---LLRTI---------XVDGTAFGAGMGLALACDYV 111 DL + A + L HR L++++ V G A AG L CD Sbjct: 68 HDLRQMQATPQQTYYE--ALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATCDLA 125 Query: 112 LCSERARFCTAYSNVGLMADMGLLWSLPQRVSAGQVRRLLYTSAVVDGEEALRLGLADQL 171 + +E ARF + NVGL + +L + + + +L T +D A GL +Q Sbjct: 126 IAAESARFAVSGINVGLFCSTPAV-ALTRNLPIKRAFEMLVTGKFIDAATAAAWGLINQA 184 Query: 172 VHSDELQQAALDLAAQFAAAPPLAVGLTKAALAR-----LPGSL---DTVMGLE-LDGQL 222 V EL A LA + + P +++ KA + R LP + VM +D + Sbjct: 185 VPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDPEA 244 Query: 223 QLFSSADFTEGRHAFLEKRRPYF 245 Q EG AFL+KR+P + Sbjct: 245 Q--------EGITAFLDKRKPNW 259 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 85.5 bits (210), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 70/241 (29%), Positives = 112/241 (46%), Gaps = 8/241 (3%) Query: 16 VTMNQPGRRNALAPEMREALTETLERLACDVDCRAIILAGTDRSFCAGGDLSSLPAGDAL 75 +T+N+P RNAL + + L R D R ++++G +++F AG DL+ + DA+ Sbjct: 22 LTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAFAAGADLNEMIHKDAI 81 Query: 76 ASRKRMTLAH-----RLLRTI--XVDGTAFGAGMGLALACDYVLCSERARFCTAYSNVGL 128 A++ + + R + I V+G A GAG L + D + + A+ NVG Sbjct: 82 ATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQPEINVGT 141 Query: 129 MADMGLLWSLPQRVSAGQVRRLLYTSAVVDGEEALRLGLADQLVHSDELQQAALDLAAQF 188 + G L + V +++ + + ++AL+ GL ++V D + L LA Sbjct: 142 LPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTLALAHSI 201 Query: 189 AAAPPLAVGLTKAA-LARLPGSLDTVMGLELDGQLQLFSSADFTEGRHAFLEKRRPYFQG 247 A PLAV L K A L L+ + E + +SAD EG AF EKR F G Sbjct: 202 AQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEKRAAVFSG 261 Query: 248 R 248 R Sbjct: 262 R 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 738,704 Number of extensions: 28773 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 8 Length of query: 248 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 165 Effective length of database: 1,226,865 Effective search space: 202432725 Effective search space used: 202432725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)