BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0230.p (358 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312970|ref|YP_003777062.1| nitrate/sulfonate/bicarbonate A... 59 4e-10 >gi|300312970|ref|YP_003777062.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 345 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 60/204 (29%), Positives = 95/204 (46%), Gaps = 18/204 (8%) Query: 8 LACTGAIAGALISIPLAVNAQAREK--VTIAIGTDVLDASQVNNTSLPIYTK----CWEN 61 L G S + AQA EK V+IA+G L LP+ +++ Sbjct: 9 LIAAGLFLAGYFSFNGSAGAQALEKPKVSIAVGGKNL------FYYLPLTIAEQLGYFKD 62 Query: 62 AGLDVTLQP-TNSTTAMQAVLTGQADMVNMGPAAAIIARTKGAPLKAVYLNMRHNFQFPV 120 GL V + A+QA++ G AD+V+ I + K P+ A L R Q + Sbjct: 63 EGLQVEISDFAGGAKALQAMVGGSADVVSGAYEHTINMQAKNQPIVAFVLQGRAP-QIVL 121 Query: 121 VLDSSPV---KTIADFKGKTIGVLSYGSQMVQIFKGMLTEAGVNPDKDVTFVETGSGAQA 177 +++ + KT+AD KGK IGV + GS + +L +AG+ P DV+F+ G+ A A Sbjct: 122 AVNNKTMPNYKTVADLKGKKIGVTAPGSSTSVMASYVLAKAGLKPS-DVSFIGVGAAAGA 180 Query: 178 VTALSKGRVDIWGTWDSQIATAEN 201 ++A+ G++D D I+ E Sbjct: 181 ISAVRSGQIDAIANLDPVISMLEQ 204 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,043,602 Number of extensions: 42050 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 2 Length of query: 358 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 272 Effective length of database: 1,212,660 Effective search space: 329843520 Effective search space used: 329843520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)