BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0222.p (276 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310325|ref|YP_003774417.1| metal ion ABC transporter perip... 134 8e-33 gi|300314030|ref|YP_003778122.1| metal ion ABC transporter perip... 127 1e-30 gi|300311120|ref|YP_003775212.1| metal ion ABC transporter perip... 119 4e-28 gi|300310887|ref|YP_003774979.1| metal ion ABC transporter perip... 113 2e-26 gi|300311637|ref|YP_003775729.1| metal ion ABC transporter perip... 102 3e-23 >gi|300310325|ref|YP_003774417.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 134 bits (337), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 98/252 (38%), Positives = 144/252 (57%), Gaps = 7/252 (2%) Query: 1 MRNRVFHRFFAALVLSSIALFAAQASAAPQKPELRVGFVPGPYIDEFKVGVQPELEKKGY 60 M+ R +F A L L++ L +A A A Q +++G GP+ E V+ LEK G Sbjct: 1 MKRRQLIQFIAGLGLAA-GLVSAPAFAQDQ---IKMGVTAGPHA-EIMEQVKKLLEKDGV 55 Query: 61 KIKYYEFSTGLEANSAVFKGDIDANVMQHSVFLKSYNERNKTDLVGIVQVPTPPMGLYSK 120 ++K EF+ ++ N+A+ GD+DAN QH +L + + V + T PMG+YSK Sbjct: 56 QMKVIEFTDYIQPNAALAAGDLDANSYQHQPYLDAQIKDRGYKFVSVGSTITFPMGVYSK 115 Query: 121 KHRSLDGLKKGFTVAVPNDPVNLQRALWILQRLGWIRIRENANPVDVTELDVVENKVGLK 180 K +SL+ LK+G V VPNDP N RAL +LQ G I+++ +A + T LD+VEN +K Sbjct: 116 KIKSLNDLKQGARVGVPNDPTNGGRALLVLQAKGVIKLKADAG-LKATPLDIVENPKKIK 174 Query: 181 LVLLENAQGPRALDDADFAAIQGNFAIFAKLK-LSEAFILEDMTPAYINVLAVKKTKQTD 239 +V L+ AQ PR+LDD D A I GN+A A L +A +E T Y NV+AV+ + Sbjct: 175 IVELDAAQLPRSLDDFDAAVINGNYAESAGLSPTKDAIAVEASTGPYANVIAVRIADKDK 234 Query: 240 QYARDIVEAYRS 251 + +V+AY S Sbjct: 235 PWVAKLVKAYHS 246 >gi|300314030|ref|YP_003778122.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 127 bits (319), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 79/262 (30%), Positives = 140/262 (53%), Gaps = 6/262 (2%) Query: 5 VFHRFFAALVLSSIALFAAQASAAPQKPELRVGFVPGPYIDEFKVGVQP-ELEKKGYKIK 63 + R + L++S +A A AA + +RVG GP+ F+ + E + GYK+K Sbjct: 7 TWLRRLSTLMVSMLASLVLNAQAAADQ-VIRVGVTSGPHAQIFEQARRVFERDNPGYKVK 65 Query: 64 YYEFSTGLEANSAVFKGDIDANVMQHSVFLKSYNERNKTDLVGIVQVPTPPMGLYSKKHR 123 EF+ ++ N+A+ G++DAN QH FL + + L + PM +YS+K+R Sbjct: 66 IIEFNDYIQPNAALDAGELDANSYQHRPFLNAQIKTRGYKLYAEGKTMIGPMAIYSRKYR 125 Query: 124 SLDGLKKGFTVAVPNDPVNLQRALWILQRLGWIRIRENANPV---DVTELDVVENKVGLK 180 L+ + G + +PNDP N R L +LQ+ G+I++R +P+ + T +D+ EN K Sbjct: 126 KLEDVPVGARIGIPNDPANESRVLLLLQKHGFIKLRAGIDPLTGTNATPIDITENPKKWK 185 Query: 181 LVLLENAQGPRALDDADFAAIQGNFAIFAKLKLS-EAFILEDMTPAYINVLAVKKTKQTD 239 V ++ AQ PR LDD D +A+ ++A A L + ++ ++E Y ++AV++ + Sbjct: 186 FVEIDAAQLPRTLDDLDASAVNADYAAKAGLNPARDSLVVESGDSPYACLIAVREKDRAQ 245 Query: 240 QYARDIVEAYRSDTFQNAIRAD 261 + +V+AY+S + I + Sbjct: 246 PWLGKLVQAYQSPEVKRYIETE 267 >gi|300311120|ref|YP_003775212.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 119 bits (297), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 93/266 (34%), Positives = 144/266 (54%), Gaps = 7/266 (2%) Query: 10 FAALVLSSIALFAAQASAAPQKPELRVGFVPGPYIDEFKVGVQPELEKKGYKIKYYEFST 69 AA+ S+AL Q A K +++VG G F V V+ + G +I+ FS Sbjct: 13 LAAIGALSLALVP-QLGQAQDKQKIKVGISVGSAEAIFDV-VKKVAARDGLEIQTVVFSD 70 Query: 70 GLEANSAVFKGDIDANVMQHSVFLKSYNERNKTDLVGIVQVPTPPMGLYSKKHRSLDGLK 129 L+ N+A+ GD+DAN QH +L+S +V + T P+G+YS+K RS++ L Sbjct: 71 YLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYSRKFRSVEQLP 130 Query: 130 KGFTVAVPNDPVNLQRALWILQRLGWIRIRENA--NPVDVTELDVVENKVGLKLVLLENA 187 +G + + NDP N RAL +LQ+ G IR++ N V+ T LDV+EN LKLV L+ A Sbjct: 131 QGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGENGVNATPLDVIENPRKLKLVELDAA 190 Query: 188 QGPRALDDADFAAIQGNFAIFAKLKLSEAFI-LEDMTPAYINVLAVKKTKQTDQYARDIV 246 Q PR+LDD A+I ++A A L L + I +ED Y N++A + + + R +V Sbjct: 191 QLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAEDKDRPWVRKLV 250 Query: 247 EAYRSDTFQNAIRADRFYDGFRLPDY 272 +AY S+ + I + + G +P + Sbjct: 251 QAYHSEEVRKFIETE--FKGSLVPAF 274 >gi|300310887|ref|YP_003774979.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 113 bits (282), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 77/274 (28%), Positives = 134/274 (48%), Gaps = 14/274 (5%) Query: 1 MRNRVFHRFFAALVLSSIALFAAQASAAPQKPELRVGFVPGPYIDEFKVGVQPELEKKGY 60 +R + H F A + S+ AA +L + P P+ E ++P L K+G Sbjct: 2 IRKLIPHLFAGAALAFSVGAHAAD--------KLVIAATPVPHA-EILEHIKPALAKEGI 52 Query: 61 KIKYYEFSTGLEANSAVFKGDIDANVMQHSVFLKSYNERNKTDL-VGIVQVPTPPMGLYS 119 ++ F+ ++ + + +D N H +L + + +K D+ V + +V P YS Sbjct: 53 DLQVKVFTDYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYS 112 Query: 120 KKHRSLDGLKKGFTVAVPNDPVNLQRALWILQRLGWIRIRENANPVDVTELDVVENKVGL 179 +K++ + + G T+A+PNDP N RAL +L R G I++++N N + T+ D+VEN L Sbjct: 113 QKYKKIADIPNGATIAIPNDPSNSGRALLLLARNGLIKLKDNTN-ISATQKDIVENPKKL 171 Query: 180 KLVLLENAQGPRALDDADFAAIQGNFAIFAKLK-LSEAFILEDMTPAYINVLAVKKTKQT 238 K LE A PR L+ D A I N+A+ AKL + ++ +ED Y N+L ++ + Sbjct: 172 KFRALEAATLPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKD 231 Query: 239 DQYARDIVEAYRSDTFQNAIRADRFYDGFRLPDY 272 + + A S + I + Y G +P + Sbjct: 232 SPAIKKLAAALNSPDVKKFI--EEKYQGAIVPAF 263 >gi|300311637|ref|YP_003775729.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 102 bits (254), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 68/201 (33%), Positives = 104/201 (51%), Gaps = 4/201 (1%) Query: 10 FAALVLSSIALFAAQASAAPQKPELRVGFVPGPYIDEFKVGVQPELEKKGYKIKYYEFST 69 F+ LV S+ + + QA+ A K LR+G VPG Y D V + E +++G ++ EF+ Sbjct: 3 FSHLVFLSLTVLSLQAAQAADK--LRIGVVPGAYGDSVAVAAK-EAKRQGIDVQVVEFTD 59 Query: 70 GLEANSAVFKGDIDANVMQHSVFLKSYNERNKTDLVGIVQVPTPPMGLYSKKHRSLDGLK 129 N A+ GD+D N QH FL + ++N L +GLYS K++SL L Sbjct: 60 WTTPNVALDAGDLDLNYFQHQPFLDNAIQKNGYKLASAGTGILSNVGLYSLKYKSLTALP 119 Query: 130 KGFTVAVPNDPVNLQRALWILQRLGWIRIRENANPVDVTELDVVENKVGLKLVLLENAQG 189 +G V + NDPVN RAL +LQ +G I+++ + + D+++N L V +E Q Sbjct: 120 QGAKVGIANDPVNQGRALLLLQNVGLIKLKPKVGYLGSID-DIIDNPRKLSFVEVEGPQL 178 Query: 190 PRALDDADFAAIQGNFAIFAK 210 R D D A +F + AK Sbjct: 179 VRITPDVDLAQGYPHFIVAAK 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 810,212 Number of extensions: 31565 Number of successful extensions: 79 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 5 Length of query: 276 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 192 Effective length of database: 1,222,130 Effective search space: 234648960 Effective search space used: 234648960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)