BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0217.p (449 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 269 3e-73 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 241 1e-64 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 61 2e-10 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 269 bits (688), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 163/423 (38%), Positives = 228/423 (53%), Gaps = 27/423 (6%) Query: 27 WRLPDADPHAFIDVGHYIRVAQIAEAAKFDAVFLADNASIIEQ----------IDFRPIT 76 WR P + F+++ +Y+ +A+ E KFD +F AD ++ + I + T Sbjct: 19 WRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVSTRYGDSHLHGLYIGDQDAT 78 Query: 77 ALEPTVLLASVAAATTHIGVIGTASTSYNEPYNIARRFATLDHVSKGRAGWNVVTTADLG 136 L+P +L ++AAAT+HIG+ T ST+Y++PY++AR FATLDH+S GRA WNVVT+ + G Sbjct: 79 RLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTSVNQG 138 Query: 137 SARNFGRDAVPNHAERYARAAEFTDVVKALWDSWEDDAVIGDKVGGRFIDTAKVQPIGHR 196 A NFG H RY RA EF +V LW SW+ DA+ + R+ D KV I H+ Sbjct: 139 EADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQQ--DRYADADKVAAIDHQ 196 Query: 197 GTHFSVHGPLNLPRTPQGHPVLVQAGGSADGRDLAARHAEAVFSASQSFEESQAYGRELN 256 G HFSV GPLN+P +PQGHPV++QAG S G+D AAR AE VF+ QA+ R+L Sbjct: 197 GAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYRDLK 256 Query: 257 ARAAAYGRGPHAVKILAGLTTIIGATEADAIRRRDELIDMIPWSYNINRLAGILGVAPER 316 RA +GR P ++IL+ + +G + A +++ + ++ L+ + V Sbjct: 257 NRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQANALADPLVGLSTLSSHMNVDFSG 316 Query: 317 IKLDERLPDDIALPSNGNGNHTFFNATLAQARQHGYTARQLVYALAG-----GGGHRVVV 371 LD L D S G A A+ RQL A AG G + Sbjct: 317 FALDAPLGD--VQVSGIQGLFKLLRAISAE--------RQLTLADAGRLYAQGVLTPQIA 366 Query: 372 GTPEQVADDIEHWFKGGAADGFNLMPDALPSGLQDFTDGVVPILQKRGIFRRDYEGTTLR 431 GT VAD ++ ADGF + P LP G DF D V+P LQ+RG FR+DY G LR Sbjct: 367 GTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQVIPELQRRGAFRQDYAGRQLR 426 Query: 432 EHL 434 +HL Sbjct: 427 QHL 429 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 241 bits (614), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 148/432 (34%), Positives = 221/432 (51%), Gaps = 18/432 (4%) Query: 13 LNINVLHSGFVPSAWRLPDADPHAFIDVGHYIRVAQIAEAAKFDAVFLADNASIIE---- 68 N+N + S W P + D+ ++ +A++ E KFD +FLAD + + Sbjct: 11 FNMNCV-SHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVGVYDVYQG 69 Query: 69 --QIDFRPITAL---EPTVLLASVAAATTHIGVIGTASTSYNEPYNIARRFATLDHVSKG 123 + R L +P +L+ ++AA T H+G TA+ +Y P+ ARR +TLDH+S+G Sbjct: 70 NTDLTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRG 129 Query: 124 RAGWNVVTTADLGSARNFGRDAVPNHAERYARAAEFTDVVKALWD-SWEDDAVIGDKVGG 182 R GWN+VT +AR G H +RY RA EF +VV LW+ SWED AV+ D+ Sbjct: 130 RVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARR 189 Query: 183 RFIDTAKVQPIGHRGTHFSVHGPLNLPRTPQGHPVLVQAGGSADGRDLAARHAEAVFSAS 242 + D AKV I H G ++ V G + Q PVL QAG S G+ ARHAE VF +S Sbjct: 190 VYADPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHAECVFISS 249 Query: 243 QSFEESQAYGRELNARAAAYGRGPHAVKILAGLTTIIGATEADAIRRRDELIDMIPWSYN 302 QS + ++ + + A GR P A+KI GLT + G TE A R E Sbjct: 250 QSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERYREYARYASPEAG 309 Query: 303 INRLAGILGVAPERIKLDERLPDDIALPSNGNGNHTFFNATLAQARQHGYTARQLVYALA 362 + + G+ + +LDE + P+ N + + + +T R+L+ L Sbjct: 310 LAHYSAGTGIDFSQYELDEPIR-----PAKTNAIESL--TKVVTQPELNWTKRKLLQQLE 362 Query: 363 GGGGHRVVVGTPEQVADDIEHWFKGGAADGFNLMPDALPSGLQDFTDGVVPILQKRGIFR 422 GG + +VG P++VAD++E W DG+NL P +DF + V+P+LQ+RG ++ Sbjct: 363 LGGRYASIVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYK 422 Query: 423 RDYEGTTLREHL 434 R+Y TLRE L Sbjct: 423 REYAQGTLREKL 434 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 59/207 (28%), Positives = 92/207 (44%), Gaps = 26/207 (12%) Query: 90 ATTHIGVIGTASTSYNEPYNIARRFATLDHVSKGRAGWNVVTTADLGSARNFGRDAVPNH 149 ATT + V+ P +A++ AT+DH+S GR NVV+ G + G + H Sbjct: 89 ATTKLKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWL-EH 147 Query: 150 AERYARAAEFTDVVKALWDSWEDDAVIGDKVGGRFIDTAKVQPIGHRGTHFSVH-GPLNL 208 ERY R+ EF +K +W +DD G F +R ++++ P+ Sbjct: 148 DERYRRSNEFIRALKGIWT--QDDFTF----KGDF----------YRFNNYTLSPKPVQK 191 Query: 209 PRTPQGHPVLVQAGGSADGRDLAARHAEAVFSASQSFEESQAYGRELNARAAAYGRGPHA 268 P HP + Q G S RD AA ++ F+ + E +A ++ A+AA G H Sbjct: 192 P-----HPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNG---HK 243 Query: 269 VKILAGLTTIIGATEADAIRRRDELID 295 V+I I TE +A ++ID Sbjct: 244 VRIGVNAFVIARDTEEEAQAVLQDIID 270 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,583,284 Number of extensions: 73763 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 3 Length of query: 449 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 361 Effective length of database: 1,203,190 Effective search space: 434351590 Effective search space used: 434351590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)