BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0206.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 434 e-123 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 110 1e-25 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 105 5e-24 gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain)... 101 8e-23 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 100 1e-22 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 434 bits (1115), Expect = e-123, Method: Compositional matrix adjust. Identities = 204/258 (79%), Positives = 231/258 (89%) Query: 1 VDLHLKDKVVIVTGGASGIGGAISLQLAAEGAIPVVFGKSQPHDAFREQLLKLQPQARFF 60 +DL+L+DKVVIVTGGASGIG AISLQLAAEGAIPVVF +S P F E+L +QP+A F Sbjct: 1 MDLNLQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPESQFWERLTAVQPRAALF 60 Query: 61 LLDLTDEGKCEQAIAETVRAFGRLDGLVNNAGVNDSVGLDAGRVAFMQSLEKNLIHYYVM 120 L+L D+ +C +A+A T+ FGRLDGLVNNAGVNDSVGL+AGR F+ SLE+NLIHYYVM Sbjct: 61 QLELQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGLEAGRDEFVASLERNLIHYYVM 120 Query: 121 AHYCVPHLKATRGAIVNVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALANDGVRVNA 180 AHYCVPHLKATRGAI+NVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAAL +DGVRVNA Sbjct: 121 AHYCVPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA 180 Query: 181 LIPAEVMTPLYEKWISGFDNPQEKIASIASKIPFGKRFTTSEEMADTAVFLLSDRAAHTT 240 LIPAEVMTPLYEKWI+ FD+PQEK+ +I SKIP GKRFTTSEEMAD AVFLLS+R++HTT Sbjct: 181 LIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTT 240 Query: 241 GQWVFVDGGYTHLDRALS 258 GQW+FVDGGYTHLDRAL+ Sbjct: 241 GQWIFVDGGYTHLDRALT 258 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 110 bits (275), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 84/250 (33%), Positives = 123/250 (49%), Gaps = 13/250 (5%) Query: 5 LKDKVVIVTGGASGIGGAISLQLAAEGAIPVVFGKSQPHDAFREQLLKLQPQARFFLL-- 62 L KV ++TGG SGIG A++L A EGA + + DA + Q A LL Sbjct: 123 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 182 Query: 63 DLTDEGKCEQAIAETVRAFGRLDGLVNNAGVNDSVGL--DAGRVAFMQSLEKNLIHYYVM 120 D+ C A+ + A+GRLD LVNNA + + + N+ + M Sbjct: 183 DVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYM 242 Query: 121 AHYCVPHLKATRGA-IVNVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALANDGVRVN 179 +P L TRGA I+N +S TA G + Y A+KGA ++ TR + LA+ G+ VN Sbjct: 243 VKEAMPFL--TRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIHVN 300 Query: 180 ALIPAEVMTPLYEKWISGFDNPQEKIASIASKIPFGKRFTTSEEMADTAVFLLSDRAAHT 239 A+ P + TPL S E++AS +P G R +E+A VFL ++ A++ Sbjct: 301 AVAPGPIWTPLIPASFSA-----EEVASFGKNVPLG-RPGQPDEVAPAYVFLATEGASYM 354 Query: 240 TGQWVFVDGG 249 TGQ + +GG Sbjct: 355 TGQVLHPNGG 364 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 105 bits (261), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 83/258 (32%), Positives = 131/258 (50%), Gaps = 24/258 (9%) Query: 3 LHLKDKVVIVTGGA--SGIGGAISLQLAAEGAIPVVFGKSQPHDAFREQLLKLQPQARFF 60 + LKDKV ++TGGA +G+G A + +A +GA VV ++ Q Sbjct: 1 MLLKDKVAVITGGAGINGLGYATARLMAEQGARVVVL--DLAQADPAAAAARIGSQHLGL 58 Query: 61 LLDLTDEGKCEQAIAETVRAFGRLDGLVNNAGVNDSV------GLDAGRVAFMQSLEKNL 114 + D+TD+ CE A A ++ FGR+D LVNNAG+ V G D R+ L+ +L Sbjct: 59 VADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRI-----LDVSL 113 Query: 115 IHYYVMAHYCVPHLKATR-GAIVNVSSKTAVTGQGNTSG--YCASKGAQLSLTREWAAAL 171 M+ +P ++A + G+I+ +SS +A G G G Y A+K L L R A Sbjct: 114 RGTLYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREF 173 Query: 172 ANDGVRVNALIPAEVMTPLYEKWISGFDNPQEKIASIASKIPFGKRFTTSEEMADTAVFL 231 +G+RVN++ P + T + + ++ +EK A IA IP R ++++ VFL Sbjct: 174 GPEGIRVNSITPGLIGTDIIKGKLT-----EEKKAEIAETIPL-ARLGRADDIGGACVFL 227 Query: 232 LSDRAAHTTGQWVFVDGG 249 SD + + TG + V+GG Sbjct: 228 GSDLSQYCTGITLDVNGG 245 >gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain) dehydrogenase type II oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 101 bits (251), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 81/258 (31%), Positives = 120/258 (46%), Gaps = 23/258 (8%) Query: 3 LHLKDKVVIVTGGASGIGGAISLQLAAEGAIPVVFGKSQPHDAFREQLLKLQPQARFFLL 62 + +K V +VTG ASG+G A + QL GA VV P + Q L ARF L Sbjct: 1 MAVKQGVYVVTGAASGLGAATAEQLIRAGA-KVVLADVNPVAGEKMQA-TLGANARFVLT 58 Query: 63 DLTDEGKCEQAIAETVRAFGRLDGLVNNAGVNDSVGL-DAGRV----AFMQSLEKNLIHY 117 D+T E ++ + G + GLVN AG+ L G V +FM++L NL+ Sbjct: 59 DVTSETSAQEVFG-VASSMGAVVGLVNCAGIAPGEKLLGKGGVHRLDSFMRALNINLVGS 117 Query: 118 YVMAHYCVPHLKA-------TRGAIVNVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAA 170 + MA H+ A RG I++ +S A GQ Y ASKG +S+T A Sbjct: 118 FNMARLAAQHISAGPADATGERGVIIHTASIAAFDGQMGQVAYAASKGGVVSMTLPMARE 177 Query: 171 LANDGVRVNALIPAEVMTPLYEKWISGFDNPQEKIASIASKIPFGKRFTTSEEMADTAVF 230 LA G+RV + P + TP+ P+E + S++ +PF R E A + Sbjct: 178 LARHGIRVVTIAPGIIETPMM------LGMPEEVLESLSRNVPFPPRLGKPSEFAGLVMH 231 Query: 231 LLSDRAAHTTGQWVFVDG 248 +L +R + G+ + +DG Sbjct: 232 ILENR--YLNGEVIRMDG 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 100 bits (249), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 80/250 (32%), Positives = 125/250 (50%), Gaps = 17/250 (6%) Query: 8 KVVIVTGGASGIGGAISLQLAAEGAIPVVF-----GKSQPHDAFREQLLKLQPQARFFLL 62 +V +VTGGA GIG +IS QLAA+G +V S+ DA L+ +A + Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADA----LVAAGHRAAPLQM 64 Query: 63 DLTDEGKCEQAIAETVRAFGRLDGLVNNAGVNDSVG-LDAGRVAFMQSLEKNLIHYYVMA 121 DL A A + +GR D LVNNAGV + LD ++Q++ N+ + Sbjct: 65 DLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAG 124 Query: 122 -HYCVPHLKATRGAIVNVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALANDGVRVNA 180 H +K G IVN+SS + + + Y SK A + LTR+ A LA G+ VN+ Sbjct: 125 QHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNS 184 Query: 181 LIPAEVMTPLYEKWISGFDNPQEKIASIASKIPFGKRFTTSEEMADTAVFLLSDRAAHTT 240 + P V TP+ + S ++ S +P +R+ T++E+A +L ++ +A+ T Sbjct: 185 VAPGPVDTPMTQAMHS-----EQTRESYYRLVPM-RRYGTTDEIASAVCYLAAEESAYIT 238 Query: 241 GQWVFVDGGY 250 G + VDGG+ Sbjct: 239 GHVIPVDGGF 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 762,246 Number of extensions: 29341 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 44 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)