BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0196.p (1023 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 431 e-121 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 397 e-111 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 329 6e-91 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 328 1e-90 gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 315 1e-86 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 431 bits (1109), Expect = e-121, Method: Compositional matrix adjust. Identities = 321/1055 (30%), Positives = 540/1055 (51%), Gaps = 84/1055 (7%) Query: 4 TRISIGNPVLATMMMMAFVVLGLFSYQRLRVDQFPDITFPVVVVQTTYPGASPENVESDV 63 +R I PV +++M+A +++G+ +Y+ L + P++ +P + V T YPGASP+ + S V Sbjct: 4 SRQFILRPVATSLLMLAILLVGIVAYRLLPLSALPEVDYPTIQVVTLYPGASPDVMTSSV 63 Query: 64 TRKIEEMVNTINGINSLTSHSYEGLSVVIVEF--DLTVDPAQGAQDVREKVALVQAAFRK 121 T +E + G+ ++S S G SV+ ++F DL++D A+ Q+V+ + Sbjct: 64 TAPLERQFGQMPGLKQMSSTSSGGASVITLQFNLDLSLDIAE--QEVQAAINAGSNLLPS 121 Query: 122 EVDQPRI-TRFDPADQPIFSISVTNDPTAPNTRPRTLRELTTISDQVIKKRLENVRGVGS 180 ++ P + ++ +PAD PI +++VT+ NT P L ++ + D I +++ + GVG Sbjct: 122 DLPMPPVYSKVNPADTPIITLAVTS-----NTMP--LPKVQDLIDTRIAQKISQLSGVGL 174 Query: 181 VTLVGGVKRQINIYVKPNEMEALGIGVDQVITAVKNENQELPTGAIRMADAEKVVQVQGR 240 V+L GG + + + + P + ALG+ +D V TA+ N N G+ + + Sbjct: 175 VSLSGGQRPAVRMQLNPRAVAALGLNLDDVRTAIGNANVNQAKGSFDGPTRASTIDANDQ 234 Query: 241 VKNPEDFKRIIVARRGSQPVTLEQIATVVDGQEEQETLALYNGQRTLALDILKAQGQNTI 300 +++ ++++ +I+A + P+ + +A VVDG E A N Q + ++I + G N I Sbjct: 235 LRSADEYRNLIIAYKNGAPIRVSDVADVVDGAENVRLGAWANTQPAVIVNIQRQPGANVI 294 Query: 301 DVADGLKQAIKDMEPNLQKLYPG-VRLQVIKDSSRQIRTGVENVRRTLLEGAALTVLIVF 359 V D +IK + P LQ PG + + ++ D + IR V++V+ LL AL V+++F Sbjct: 295 AVVD----SIKKILPQLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSVALVVMVIF 350 Query: 360 LFLNSWRSTVITGLTLPVALIGTFLFMYMFGFTINMITLMALSLCVGLLIDDAIVVRENI 419 +FL + +T+I + +P++L+GTF MY+ GF+IN +TLMAL++ G ++DDAIV+ ENI Sbjct: 351 VFLRNVPATIIPSVAVPLSLVGTFAIMYLAGFSINNLTLMALTIATGFVVDDAIVMIENI 410 Query: 420 VRH--AGMKVHGKFKTHKMAALEGTAEIGLAVLATTFSIVAVFLPVGFMGGIIGRFFHQF 477 R+ GMK AAL+G +IG +++ TFS++AV +P+ FMG ++GR FH+F Sbjct: 411 SRYIEEGMK-------PMQAALKGAEQIGFTIISLTFSLIAVLIPLLFMGDVVGRLFHEF 463 Query: 478 GITVTAAVLISMFVSFTLDPMLSSIWPDPDVHGEKRKDGWYAKSIGRVLDQFERLVQWLS 537 +T+ ++LIS VS TL PM+ + H + + GW+ + F+R++ Sbjct: 464 AVTLAVSILISAVVSLTLTPMMCARLLR---HQPEEQQGWFYHKSQQF---FDRII---- 513 Query: 538 ASYQKLLKWSLRHRIMTLVLALLSFIAGLALPAAGLIGSEFVPQADYSE-SGVTFYTPVG 596 A Y +L+W L + +TL++A + GL + + F P D G++ T Sbjct: 514 ARYGVMLQWVLARQKLTLLVAAATL--GLTVLLYVFVPKGFFPLQDTGVIQGISEATQSI 571 Query: 597 SSLELTESKVHQVETVLRQFPEVVDTYSTVNT-GNSQGKNYATVFIRLKPRKERQLSTAQ 655 S + E K Q+ V+ Q P V S + G + N + I LKP R++S Sbjct: 572 SFSAMGE-KQQQLAKVVLQDPAVESLTSFIGVDGTNATLNSGRMLINLKPHDVREVSATD 630 Query: 656 LAPQLRERL-TRVAGITVTHIGTLDGVGGDN---KQVRFSLLGPDLDQLGRLSEEVQTRL 711 + +L+ +L V GIT+ D D+ Q +F+L D L ++ RL Sbjct: 631 VIRRLQGKLAAEVPGITLYMQPVQDLTIEDSVSRTQYQFTLEDADPAVLSTWVPKLIARL 690 Query: 712 RAIPGVADLDSSLKPQKPTISIEPKRDIGSDLGVGVAQIGNAL----------------- 754 + +AD+ ++L+ Q IE R+ S LG+ A I NAL Sbjct: 691 QQQSELADVATNLQDQGLQAYIEIDRNAASRLGITTAAIDNALYNAFGQRLISTIYTQSN 750 Query: 755 --------RPNNTDGTPRM------------VPLRQVADIRNTVGANQINRRDLNREVEL 794 +P G + VPL +A + +N + Sbjct: 751 QYRVVMEVKPEFQHGPAALDSIYLVTSGGGKVPLSSIATVTERTAPLVVNHIGQFPAATV 810 Query: 795 SAN-AAGRSAGQVNADIKVMLDKINWPPGYRYQIGGSTKSMNESFGYALGALALAIVFIY 853 S N A G S G+ IK ++I P G+ + S L + AIV +Y Sbjct: 811 SFNLAKGASLGEAVKAIKAAEEEIGMPASTVTNFQGAALAFQASLSNTLWLILAAIVTMY 870 Query: 854 MILASQFASFLQPVAIMSSLPLTLIGVFLALMFFRSTLNMFSIIGFIMLMGLVTKNAILL 913 ++L + S++ P+ I+S+LP +G L+LM + L + IIG I+L+G+V KNAI++ Sbjct: 871 IVLGVLYESYIHPITILSTLPSAGVGALLSLMISGTDLGIIGIIGIILLIGIVKKNAIMM 930 Query: 914 VDFANQA-RKEGMERGAALLEAAHVRLRPILMTTLAMVFGMVPLAFGIAEGSEQRAPMGQ 972 +DFA +A R EG A+ +A +R RPILMTT+A + G +PL G GSE R P+G Sbjct: 931 IDFALEAERHEGKSPREAIYQACLLRFRPILMTTMAALLGALPLMLGTGVGSELRQPLGI 990 Query: 973 AVIGGIITSSLLTLVVVPVAYTYLDDLGAWVKRKW 1007 ++GG++ S +LTL PV Y D LG ++ ++ Sbjct: 991 TMVGGLLVSQVLTLFTTPVIYLGFDSLGRRLRARF 1025 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 397 bits (1019), Expect = e-111, Method: Compositional matrix adjust. Identities = 306/1066 (28%), Positives = 526/1066 (49%), Gaps = 106/1066 (9%) Query: 8 IGNPVLATMMMMAFVVLGLFSYQRLRVDQFPDITFPVVVVQTTYPGASPENVESDVTRKI 67 I P+ T++ +A +++G+ + L V P + FP + V PG SPE + S+V + + Sbjct: 8 IKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMASNVAQPL 67 Query: 68 EEMVNTINGINSLTSHSYEGLSVVIVEFDLTVDPAQGAQDVREKVALVQAAFRKEV-DQP 126 E + I G++ +TS S G + + ++FDL + A DV+ + + + P Sbjct: 68 ERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLPANLPNPP 127 Query: 127 RITRFDPADQPIFSISVTNDPTAPNTRPRTLRELTTISDQVIKKRLENVRGVGSVTLVGG 186 + +PAD PI +SV ++ P T+ + +D ++ +++ + GVG V + G Sbjct: 128 TFRKINPADSPIMIMSVQSE-----ALPLTV--VNDYADNILAQQISQISGVGLVNVNGQ 180 Query: 187 VKRQINIYVKPNEMEALGIGVDQVITAVKNENQELPTGAIRMADAEKVVQVQGRVKNPED 246 K + I V P ++ LG+ ++ + + P G + A V ++ E Sbjct: 181 QKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQ 240 Query: 247 FKRIIVARRGSQPVTLEQIATVVDGQEEQETL-------------ALYNGQRTLALDILK 293 + +++A R P+ ++ I VDG E + ++ NG R++ L I K Sbjct: 241 WNDMVLAWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNG-RSIVLAITK 299 Query: 294 AQGQNTIDVADGLKQAIKDMEPNLQKLYPG-VRLQVIKDSSRQIRTGVENVRRTLLEGAA 352 G N I+ +K A+ P L+ P + + + D ++ IR VE+V TL+ A Sbjct: 300 QPGANVIETVGRIKAAM----PRLRAAIPAAIHVNTLIDRTQTIRASVEDVEFTLVLTIA 355 Query: 353 LTVLIVFLFLNSWRSTVITGLTLPVALIGTFLFMYMFGFTINMITLMALSLCVGLLIDDA 412 L V+++F+FL + +T+I +T+P+A++GT MY+ G++++ ++LMAL++ VG ++DDA Sbjct: 356 LVVMVIFVFLRNVAATLIPSVTVPLAIMGTAGVMYVVGYSLDNLSLMALTIAVGFVVDDA 415 Query: 413 IVVRENIVRHA--GMKVHGKFKTHKMAALEGTAEIGLAVLATTFSIVAVFLPVGFMGGII 470 IV+ ENI R+ GM T AAL+G EIG +++ + S+VAVF+P+ MGGI+ Sbjct: 416 IVMLENIYRYVEEGM-------TPLEAALKGAGEIGFTIISISVSLVAVFIPLLLMGGIV 468 Query: 471 GRFFHQFGITVTAAVLISMFVSFTLDPMLSSIWPDPDVHGEKRKDGWYAKSIGRVLDQFE 530 GR F +F +TVT + +S+ +S TL PML S + + HG++ G++ FE Sbjct: 469 GRLFREFAVTVTLTIAVSVIISLTLTPMLCSRFLKNE-HGQRH---------GKLYQWFE 518 Query: 531 RLVQWLSASYQKLLKWSLRHRIMTLVLALLSFIAGLALPAAGLIGSEFVPQADYSESGVT 590 R + A Y++ L ++H+ +T L+SFIA LAL A I F+P+ + + Sbjct: 519 RCFDAMLAGYKRGLDVVMKHQFIT----LMSFIATLALTAVLFI---FIPKGFFPQQDNG 571 Query: 591 FYTPVGSSLELTES-----KVHQVETVLRQFPEVVDTYSTVNTGNSQGKNYATVFIRLKP 645 S + T S ++ +V V+RQ P+V+ + S N FI LKP Sbjct: 572 VIVGFAESAQDTSSAAMHERLLKVAEVVRQDPDVI---GFAMSAGSTTFNTGQFFIALKP 628 Query: 646 RKERQLSTA-QLAPQLRERLTRVAGITVTHIGTLD-GVGG--DNKQVRFSLLGPDLDQLG 701 + + + + A ++ +LR ++ R+ G+ + + D VGG Q ++++ DLD+L Sbjct: 629 KDQGRTTNADEIIARLRPKVARIEGVNLFMQASQDINVGGRLSRTQYQYTITDTDLDELN 688 Query: 702 RLSEEVQTRLRAIPGVADLDSSLKPQKPTISIEPKRDIGSDLG-------------VGVA 748 + + R R +P + D+ S + Q I R S G +G Sbjct: 689 VWAPRMLERFRQLPQLTDVASDQQNQAAAAVITIDRARASSFGISPSLIDATIYDAIGQR 748 Query: 749 QIGNALRPNNT-----DGTPRM--------------------VPLRQVADIRNTVGAN-Q 782 Q+ N+ + TP + VPL I + + Sbjct: 749 QVAQYFTQINSYRVILEVTPALQKDPALFDKLYLTSPLTGQQVPLSTFVKIDTSKTSYLS 808 Query: 783 INRRDLNREVELSAN-AAGRSAGQVNADIKVMLDKINWPPGYRYQIGGSTKSMNESFGYA 841 I+ + V +S N A G S G+ I+ D++ P G+ K+ +S Sbjct: 809 ISHQSQFPAVTISFNLAKGVSLGEAVDVIQKTKDQMGVPQTLVGNFQGTAKAFGDSLSSQ 868 Query: 842 LGALALAIVFIYMILASQFASFLQPVAIMSSLPLTLIGVFLALMFFRSTLNMFSIIGFIM 901 +A A++ +Y++L + S++ P+ I+S+LP +G L LM L++ ++IG I+ Sbjct: 869 PYLIAAALIAVYIVLGLLYESYIHPLTILSTLPSAGVGALLILMAGGYDLSVIALIGIIL 928 Query: 902 LMGLVTKNAILLVDFANQA-RKEGMERGAALLEAAHVRLRPILMTTLAMVFGMVPLAFGI 960 L+G+V KN I+++DFA A R +GM+ A+ +A +R RPI+MTT+ + +PL G Sbjct: 929 LIGIVKKNGIMMIDFALTAERSQGMKPEQAIYQACLLRFRPIMMTTMCALLSGLPLMLGH 988 Query: 961 AEGSEQRAPMGQAVIGGIITSSLLTLVVVPVAYTYLDDLGAWVKRK 1006 GSE R P+G A++GG+I S LTL PV Y YLD W RK Sbjct: 989 GAGSELRRPLGYAMVGGLILSQALTLFTTPVVYLYLDRAHYWYMRK 1034 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 329 bits (844), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 291/1094 (26%), Positives = 514/1094 (46%), Gaps = 131/1094 (11%) Query: 5 RISIGNPVLATMMMMAFVVLGLFSYQRLRVDQFPDITFPVVVVQTTYPGASPENVESDVT 64 + I PV A ++ + ++ G + L V Q+P+I P +VV TYPGA+ + ++ VT Sbjct: 3 KFFIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDSVT 62 Query: 65 RKIEEMVNTINGINSLTSHSY-EGLSVVIVEFDLTVDPAQGAQDVREKVALVQAAFRKEV 123 IE+ +N +G+ + S S G + + F +P A DV+ ++ V+A V Sbjct: 63 SLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPAAV 122 Query: 124 DQPRITRFDPADQPIFSISVTNDPTAPNTRPRTLRELTTISDQVIKKRLENVRGVGSVTL 183 Q + + I +++ T P L + +S V+ + ++ V GVG L Sbjct: 123 TQQGVQVTKSRSNFLLFIGLSS--TDGKLDPVALGDY--LSRNVLNE-IKRVPGVGQAQL 177 Query: 184 VGGVKRQINIYVKPNEMEALGIGVDQVITAVKNENQELPTGAIRMADAEKVVQ------V 237 G +R + I++ PN++ L + V A++ +N + G + + Q V Sbjct: 178 FG-TERAMRIWIDPNKLIGLNLTPADVTAAIQAQNALVAGGTLGALPSPGTQQIAATVVV 236 Query: 238 QGRVKNPEDFKRIIV-ARRGSQPVTLEQIATVVDGQEEQETLALYNGQRTLALDILKAQG 296 G++ +PE F II+ A V L +A V G + +T +G+ + + + + Sbjct: 237 NGQLTSPEQFGNIILRANANGATVRLRDVARVEVGGQSYDTSGRLDGKPSSFIGVQLSPT 296 Query: 297 QNTIDVADGLKQAIKDMEPNLQKLYP-GVRLQVIKDSSRQIRTGVENVRRTLLEGAALTV 355 N + A + K ME L K +P GV+ + D+S+ ++ +E V +TL+E L Sbjct: 297 ANALGTATAVH---KKME-ELAKYFPTGVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVF 352 Query: 356 LIVFLFLNSWRSTVITGLTLPVALIGTFLFMYMFGFTINMITLMALSLCVGLLIDDAIVV 415 L++FLFL + R T+I + +PVAL+GTF + +FGF+IN++T+ + L +G+L+DDAIVV Sbjct: 353 LVMFLFLQNIRYTIIPTIVVPVALLGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIVV 412 Query: 416 RENIVRHAGMKVHGKFKTHKMAALEGTAEIGLAVLATTFSIVAVFLPVGFMGGIIGRFFH 475 EN+ R M G + A + +I A++ T ++AVF+P+ F GG +G + Sbjct: 413 VENVERI--MSEEG--LSPADATRKAMGQISGALIGITLVLIAVFIPMAFFGGAVGAIYR 468 Query: 476 QFGITVTAAVLISMFVSFTLDPMLSS--IWPDPDVHGEKRKD--GWYAKSIGRVLDQFER 531 QF +++ A+++ S+ ++FTL P L + + P H ++K GW+ + ++ Sbjct: 469 QFSLSMVASMVFSVLMAFTLTPALCATILKPVEKGHHHEKKGFFGWFNRKFNSTASGYQG 528 Query: 532 LVQWLSASYQKLLKWSLRHRIMTLVLALLSFIAGLALPAAGLIGSEFVPQADYSESGVTF 591 ++ +L + R+ I+ +++ + + LPA+ F+P D Sbjct: 529 II-------GGMLNKAGRYMIVYVLIVACVGLLYVRLPAS------FLPNEDQGYIITNV 575 Query: 592 YTPVGSSLELTESKVHQVETVLRQFPEVVDTYSTVNTGNS-QGKNYATVFIRLKPRKERQ 650 P G+S T + V Q+E + P V + + S G+N A VF LK +R Sbjct: 576 QLPPGASTSRTTAVVKQIEEYYLKDPAVEHAIAVLGYSFSGNGQNAALVFTPLKDWSQRD 635 Query: 651 LSTAQLAPQLRERLTRVAGITVTHIGTLDGVGGDNKQVRFSLLGPDLDQLG-------RL 703 S + A +AG G + + F L P + +LG RL Sbjct: 636 KSQSADA---------IAG---KAFGAFSQI---KDAIVFPLNPPPIPELGNATGFTFRL 680 Query: 704 SEE--------VQTRLRAIPGVADLDSSL---KPQ----KPTISIEPKRDIGSDLGVGVA 748 + VQ R I G+A L +P+ P + ++ RD + LGV + Sbjct: 681 QDRAGLGHTALVQAR-NQILGMASQSKVLAGVRPEGLEDAPQLQLDIDRDKANALGVPFS 739 Query: 749 QIGNALR-----------PN----------------------------NTDGTPRMVPLR 769 I + L PN N+ GT MVP Sbjct: 740 TINSVLSTALGSSYVNDFPNLGRQQRVIVQADAKRRLQPEDLTNLYARNSSGT--MVPFS 797 Query: 770 QVADIRNTVGANQINRRDLNREVELSANAA-GRSAGQVNADIKVMLDKINWPPGYRYQIG 828 + G Q+ R + ++++ AA GRS G+ A+++ + K+ PPG+ ++ Sbjct: 798 AFVTAKWINGPVQLIRYNGYPAMKIAGGAAPGRSTGEAMAEMESFVAKL--PPGFGFEWT 855 Query: 829 GSTKSMNESFGYALGALALAIVFIYMILASQFASFLQPVAIMSSLPLTLIGVFLALMFFR 888 G + S A AL+++ ++++LA+ + S P+A+M +PL ++G L + Sbjct: 856 GQSLEEKTSGNQAPALYALSLIAVFLVLAALYESTSIPLAVMLVVPLGILGALLGVTLRA 915 Query: 889 STLNMFSIIGFIMLMGLVTKNAILLVDFANQARKEGMERGAALLEAAHVRLRPILMTTLA 948 +++ +G I ++GL KNAIL+++FA + +G A LEA H+R RPI+MT+LA Sbjct: 916 MPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQGKGLIEATLEAVHLRFRPIIMTSLA 975 Query: 949 MVFGMVPLAFGIAEGSEQRAPMGQAVIGGIITSSLLTLVVVPVAYTYLDDLGAWVKRKWF 1008 + G++PLA GS + +G V+GG+IT+++L + +VP+ + V RK F Sbjct: 976 FILGVLPLAIASGAGSASQRAIGTGVMGGMITATVLAVFLVPIFFV--------VVRKIF 1027 Query: 1009 GAAVVEDKAEDEHA 1022 + + K + EH+ Sbjct: 1028 KGSERQRKFDAEHS 1041 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 328 bits (841), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 278/1078 (25%), Positives = 501/1078 (46%), Gaps = 125/1078 (11%) Query: 5 RISIGNPVLATMMMMAFVVLGLFSYQRLRVDQFPDITFPVVVVQTTYPGASPENVESDVT 64 R I P+ A ++ + V+ G+ + +L + ++P + P V + TYPGA+P+ + V Sbjct: 3 RFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDGVI 62 Query: 65 RKIE-EMVNTINGINSLTSHSYEGLSVVIVEFDLTVDPAQGAQDVREKVALVQAAFRKEV 123 IE E+ N + +S G + + V F DP DV+ K+ ++A + V Sbjct: 63 SLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQTV 122 Query: 124 DQPRITRFDPADQPIFSISVTNDPTAPNTRPRTLRELTTISDQVIKKRLENVRGVGSVTL 183 Q +T A + +S+T+ + R +R ++ ++++ L + GVG V L Sbjct: 123 RQNGVTVESAASSFLLMVSLTSV----DGRYDEVRLNDYLARNIVEE-LRRMEGVGRVQL 177 Query: 184 VGGVKRQINIYVKPNEMEALGIGVDQVITAVKNENQELPTGAIRMADAEKV--------V 235 G ++ + I++ P ++ A G+ V + TA+ +N ++ G R+ DA V + Sbjct: 178 FGA-EQAMRIWLDPFKLNAYGLTVTDISTAISQQNAQIAPG--RLGDAPAVRGQQIAMPL 234 Query: 236 QVQGRVKNPEDFKRIIV-ARRGSQPVTLEQIATVVDGQEEQETLALYNGQRTLALDILKA 294 VQG++++P F I++ A+ V L +A V G + NG+ + + Sbjct: 235 TVQGQLQSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLS 294 Query: 295 QGQNTIDVADGLKQAIKDMEPNLQKLYPGVRLQVIKDSSRQIRTGVENVRRTLLEGAALT 354 G N + A ++ ++++ P+L + G+ + +++ ++ ++ V +TL+E L Sbjct: 295 PGANAVRTAAAIQARMEELRPSLPQ---GMAFSLPYNTAPFVKISIQKVVQTLIEAMVLV 351 Query: 355 VLIVFLFLNSWRSTVITGLTLPVALIGTFLFMYMFGFTINMITLMALSLCVGLLIDDAIV 414 L+++LFL + R TVI + P+AL+GT M + G+++N++T+ + L +G+++DDAIV Sbjct: 352 FLVMYLFLQNVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGIIVDDAIV 411 Query: 415 VRENIVR---HAGMKVHGKFKTHKMAALEGTAEIGLAVLATTFSIVAVFLPVGFMGGIIG 471 V EN+ R H G+ + A ++ EI A++ T + AVF+P+ GG +G Sbjct: 412 VVENVERIMAHEGLG-------PREATIKAMGEITGAIIGITLVLTAVFIPMALAGGSVG 464 Query: 472 RFFHQFGITVTAAVLISMFVSFTLDPMLSSIWPDPDVHGEKRKDGWYAKSIGRVLDQFER 531 + QF + + ++L S F++ +L P L + P G K G++ G F R Sbjct: 465 VIYRQFSVAMAVSILFSAFLALSLTPALCATLLRPVPAGHHDKRGFF----GAFNRAFNR 520 Query: 532 LVQWLSASYQKLLKWSLRHRIMTLVLALLSFIAGLALPAAGLIGSEFVPQADYSESGVTF 591 L ++ +L+ LR ++ L+ L + ALP+A F+P+ D +F Sbjct: 521 LTARYESALGRLIARGLRMMLVYAALSGLLVVGMQALPSA------FLPEEDQGYFMTSF 574 Query: 592 YTPVGSSLELTESKVHQVETVLRQFPEVVDTYSTVNTGNS-QGKNYATVFIRLKPRKERQ 650 P ++ E T V Q E L+ ++ S + G S G N A F LK KER+ Sbjct: 575 QLPADATTERTARAVDQFERYLKGREDIGANLSVLGFGFSGSGPNAAMAFTELKDWKERK 634 Query: 651 LSTAQLAPQLRERLTRVAGITVTHIGTLDGVGGDNKQVRFSLLGPDLDQLG-------RL 703 ST+ GV G + SLL P +++LG RL Sbjct: 635 GSTSAAE------------AMAAQAAMESGVDG----MVMSLLPPAIEELGNSSGFAMRL 678 Query: 704 SEEVQ-----------------TRLRAIPGVADLDSSLKPQKPTISIEPKRDIGSDLGVG 746 + + R + GV S P ++ +E R + LGV Sbjct: 679 QDRANQGYAALKAAEAQLLALAAKSRKLSGVY---SDGLPAGASVQLEIDRHKAAALGVS 735 Query: 747 VAQIGNALR-----------PN---------NTDGTPR-----------------MVPLR 769 + I + L PN + R MVPL Sbjct: 736 FSSISDTLSAAMGSFYVNDFPNAGRMQQVILQAQASARMQLEDVMKLYVRNQSGGMVPLA 795 Query: 770 QVADIRNTVGANQINRRDLNREVELSANAA-GRSAGQVNADIKVMLDKINWPPGYRYQIG 828 ++ Q+ R V +S +AA G ++G+ A+++ + ++ PPG+ Sbjct: 796 ELVKASWREAPTQLQRYQGFPAVRISGSAAPGVASGEAMAEMERLAQQL--PPGFAVAWT 853 Query: 829 GSTKSMNESFGYALGALALAIVFIYMILASQFASFLQPVAIMSSLPLTLIGVFLALMFFR 888 G + S A +AL+++ ++++LA+ + S+ P+++M +PL L+G A++ Sbjct: 854 GQSLQERLSAAQAPMLMALSMLVVFLVLAALYESWSIPLSVMLVVPLGLVGAVAAVILRD 913 Query: 889 STLNMFSIIGFIMLMGLVTKNAILLVDFANQARKEGMERGAALLEAAHVRLRPILMTTLA 948 ++F +G I ++GL KNAIL+V+FA Q R EGM A ++A+ +RLRPILMT+LA Sbjct: 914 MPNDVFFKVGMITVIGLSAKNAILIVEFARQLRSEGMGLVEAAIKASVLRLRPILMTSLA 973 Query: 949 MVFGMVPLAFGIAEGSEQRAPMGQAVIGGIITSSLLTLVVVPVAYTYLDDLGAWVKRK 1006 G+VPL +E + +G V GG++++++L ++ VPV + + + RK Sbjct: 974 FALGVVPLMLASGASAETQRAIGTGVFGGMVSATVLAVLFVPVFFVLVLGCAQRLARK 1031 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 315 bits (807), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 262/1052 (24%), Positives = 479/1052 (45%), Gaps = 109/1052 (10%) Query: 5 RISIGNPVLATMMMMAFVVLGLFSYQRLRVDQFPDITFPVVVVQTTYPGASPENVESDVT 64 R +I + L + ++ G++SYQ+L +D PDIT V + T+ PG SP E ++ Sbjct: 7 RFAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLEAEQRIS 66 Query: 65 RKIEEMVNTINGINSLTSHSYEGLSVVIVEFDLTVDPAQGAQDVREKVALVQAAFRKEVD 124 IE +++ + + + S S GLS V V F D Q V E++ L ++ +D Sbjct: 67 YPIETLMSGLPNLEQVRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQLARSQLPPGID 126 Query: 125 QPRITRFDPADQPI---------FSISVTNDPTAPNTRPRTLRELTTISDQVIKKRLENV 175 P PI +++ +D P+ P T +L I D VIK +L V Sbjct: 127 --------PGMGPISTGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWVIKPQLRLV 178 Query: 176 RGVGSVTLVGGVKRQINIYVKPNEMEALGIGVDQVITAVKNENQELPTGAIRMADAEKVV 235 GV + +GG ++ + ++ + G+ +D +I A++ N + G I + ++ Sbjct: 179 PGVTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIERRGEQYLI 238 Query: 236 QVQGRVKNPEDFKRIIVARRGSQPVTLEQIATVVDGQEEQETLALYNGQRTLALDILKAQ 295 + G+V + +D I++A P+ + +A V G+E + A NG+ + + Sbjct: 239 RAPGQVASIDDIGNIVLANVKGVPIRIRDVAEVEIGRELRTGAATENGREVVLGTVFMLI 298 Query: 296 GQNTIDVADGLKQAIKDMEPNLQKLYPGVRLQVIKDSSRQIRTGVENVRRTLLEGAALTV 355 G+N+ V+ + + ++++ +L K GV + D + + + V++ L+EGA L V Sbjct: 299 GENSRKVSQAVARKMEEINRSLPK---GVEAVTVYDRTVLVDKAIATVKKNLVEGALLVV 355 Query: 356 LIVFLFLNSWRSTVITGLTLPVALIGTFLFMYMFGFTINMITLMALSLCVGLLIDDAIVV 415 ++F+FL + R+ +IT + +P+A++ TF M + N+++L AL G++ID A+V+ Sbjct: 356 AVLFVFLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGALDF--GIIIDGAVVI 413 Query: 416 RENIVR-------HAGMKVHGKFKTHKMAALEGTAEIGLAVLATTFSIVAVFLPVGFMGG 468 EN VR H G + + H++ A E ++ I+ V+LP+ + G Sbjct: 414 VENCVRRLAHAQAHHGRTLTRSERFHEVFA--AAKEARRPLIFGQLIIMIVYLPMFALTG 471 Query: 469 IIGRFFHQFGITVTAAVLISMFVSFTLDPMLSSIWPDPDVHGEKRKDGWYAKSIGRVLDQ 528 + G+ F TV AA++ +M +S T P +++ GEK V ++ Sbjct: 472 VEGKMFTPMAFTVVAALIGAMILSVTFIPAAIALF-----IGEK------------VAEK 514 Query: 529 FERLVQWLSASYQKLLKWSLRHRIMTLVLA-LLSFIAGLALPAAGLIGSEFVPQADYSES 587 L+ W Y+ +LK + ++ + L A L + G+ A +GSEFVP + + Sbjct: 515 ENILMLWAKRLYEPMLKTIMENKPVVLTFAGALILVCGV---VASRLGSEFVPSLNEGDM 571 Query: 588 GVTFYTPVGSSLELTESKVHQVETVLRQ-FPEVVDTYSTVNTG----NSQGKNYATVFIR 642 + G+SL + Q+E L+Q FPEV ++ T + N + +I Sbjct: 572 AIQALRIPGTSLSQSLEMQKQIEKTLKQKFPEVERVFARTGTAEVASDPMPPNISDGYIM 631 Query: 643 LKPRK---ERQLSTAQLAPQLRERLTRVAGITVTHIGTL-----DGVGGDNKQVRFSLLG 694 LKP++ E + S +L +++ + ++ G + + + + G V + G Sbjct: 632 LKPQEQWPEPKKSREELLEAIQDEVAKIPGNSYEFSQPIQLRFNELISGVRSDVAVKVFG 691 Query: 695 PDLDQLGRLSEEVQTRLRAIPGVADLDSSLKPQKPTISIEPKRDIGSDLGVGVAQIGN-- 752 D+ L + ++ L IPG ++ P +++E R+ + G+ V+ I + Sbjct: 692 DDMKVLNDTASKISAVLEKIPGATEVKIEQTTGLPMLTVEIDREKTARYGLNVSDIQDTV 751 Query: 753 -----------------------------------------ALRPNNTDGTPRMVPLRQV 771 AL P VPL +V Sbjct: 752 ATAIGGKEAGTMFQGDRRFGIVVRLPDQARTDLEAIRRLPIALPPQEGATRVSYVPLGEV 811 Query: 772 ADIRNTVGANQINRRDLNREVELSANAAGRSAGQVNADIKVMLDK-INWPPGYRYQIGGS 830 A + G NQI+R D R + +SAN GR G A+ + L + P GY GG Sbjct: 812 ASLTIAPGPNQISREDGKRRIVVSANVRGRDLGGFVAEAERQLQSAVKVPAGYWTTWGGQ 871 Query: 831 TKSMNESFGYALGALALAIVFIYMILASQFASFLQPVAIMSSLPLTLIGVFLALMFFRST 890 +++ + + +A++ ++++L + F++ + + + +P L G LAL Sbjct: 872 FENLQSATQRLQIVIPVALLMVFVLLFAMFSNVKDGLLVFTGIPFALTGGILALAMRGIP 931 Query: 891 LNMFSIIGFIMLMGLVTKNAILLVDFANQARKEGMERGAALLEAAHVRLRPILMTTLAMV 950 +++ + +GFI L G+ N ++++ F R+EG AA+ E A RLRP+LMT L Sbjct: 932 MSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRTLDAAIHEGAMTRLRPVLMTALVAS 991 Query: 951 FGMVPLAFGIAEGSEQRAPMGQAVIGGIITSS 982 G VP+A G+E + P+ VIGGI++S+ Sbjct: 992 LGFVPMALATGTGAEVQRPLATVVIGGILSST 1023 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,977,320 Number of extensions: 122822 Number of successful extensions: 542 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 478 Number of HSP's successfully gapped: 21 Length of query: 1023 Length of database: 1,619,870 Length adjustment: 95 Effective length of query: 928 Effective length of database: 1,170,045 Effective search space: 1085801760 Effective search space used: 1085801760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)