BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0191.p (410 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 588 e-169 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 347 1e-96 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 338 4e-94 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 183 2e-47 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 154 2e-38 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 588 bits (1515), Expect = e-169, Method: Compositional matrix adjust. Identities = 301/412 (73%), Positives = 347/412 (84%), Gaps = 12/412 (2%) Query: 1 MSSVSRFTRLTLTVVAAAALAACGQKPNAQAGAGMPPGEVSVVTIAPERVTISNELPGRL 60 MSSVSRFTRLT+TV AAAL ACGQKP AQ G MPP EVSVVTIAP+RV I+NELPGR+ Sbjct: 1 MSSVSRFTRLTMTVAIAAALVACGQKPAAQGGP-MPPSEVSVVTIAPQRVAITNELPGRV 59 Query: 61 EATRVAEVRARVAGIILKRTFREGSEVKAGEVLYRIDPATFKANYDSALATLAKSQASLT 120 EA RVA+VRARVAGI+ KR F+EGSEVK G+VL++IDPA +KA+ +SA ATLA++QA+LT Sbjct: 60 EAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLT 119 Query: 121 QASLKAQRYKPLVEVNAVSKQEYDDAVAAQKQAEADVAAGKAAVQTAGLSLGYATVTSPI 180 Q +L AQRYKPLVEVNAVSKQEYD+AVAAQKQAEADVAAGKAAVQTA L+LGYATVTSPI Sbjct: 120 QTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPI 179 Query: 181 SGRIGRALVTEGALVGQGDATQLAVVQQLDPIYVNLTQSSTDLLKLQQAMASGQLKTVGQ 240 SGRIGRALVTEGALVGQGDATQLA+VQQLDP+YVN+TQSS DLLKLQQAMA+GQLK+VG Sbjct: 180 SGRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGD 239 Query: 241 GKAKVTLVTDDGRPYPLPGKLLFSDVTVDATTGAVTIRAEFPNPNRTLLPGMYVRAQLEQ 300 KA VTL ++G+ YPLPGKLLFSD++VD TG+V++RAEFPNP R LLPG YVRA++EQ Sbjct: 240 NKASVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQ 299 Query: 301 AVNESAIVVPQQAVMRDLNGSSVFVVGDDNKVAARPVKTGSAQGNSWIVTDGLKAGDRVI 360 AV+E A++VPQQAV+RD GS+V VV +NKV RPVKTG A+GN+W V DGLK GDRVI Sbjct: 300 AVDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVI 359 Query: 361 VEGIQKTKPGAPVKPVPWKGPAADAGAAPAGASAAPGRAPAKQDAAT--KAN 410 VEG+QK +PGA VKPV W PAGA A PAKQ+A+ KAN Sbjct: 360 VEGLQKVRPGATVKPVQWN---------PAGAPAQASGQPAKQEASAQPKAN 402 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 347 bits (890), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 189/362 (52%), Positives = 254/362 (70%), Gaps = 3/362 (0%) Query: 18 AALAACGQKPNAQAGAGMPPGEVSVVTIAPERVTISNELPGRLEATRVAEVRARVAGIIL 77 + L+ACG+ P A P + VVT+ + V + ELPGR ++A+VR +V GII Sbjct: 20 SVLSACGKPPGGAPPAAGTP-VLGVVTVHAQPVELHTELPGRTSPYQIADVRPQVNGIIK 78 Query: 78 KRTFREGSEVKAGEVLYRIDPATFKANYDSALATLAKSQASLTQASLKAQRYKPLVEVNA 137 R+FREGS+VKAG+VLY+I+PA+++A YDS++A LAK++AS+ A LKA RYK LV + A Sbjct: 79 ARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELVAIKA 138 Query: 138 VSKQEYDDAVAAQKQAEADVAAGKAAVQTAGLSLGYATVTSPISGRIGRALVTEGALVGQ 197 VS+Q+YDDAVA+ +AEADV + +A VQT+ ++L YA + +PISGRIG++ VT GALV Sbjct: 139 VSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGALVTA 198 Query: 198 GDATQLAVVQQLDPIYVNLTQSSTDLLKLQQAMASGQLKTVGQGKAKVTLVTDDGRPYPL 257 T L +QQLDPIYV++TQSST L L++A+A+GQL+ G G AKV LV ++GR Y Sbjct: 199 NQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSGNG-AKVRLVLENGRTYAQ 257 Query: 258 PGKLLFSDVTVDATTGAVTIRAEFPNPNRTLLPGMYVRAQLEQAVNESAIVVPQQAVMRD 317 G L FSDVTV+ TGA+T+RA FPNP LLPGMYVRA L++ V + ++VPQQAV RD Sbjct: 258 EGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAVSRD 317 Query: 318 LNGSSV-FVVGDDNKVAARPVKTGSAQGNSWIVTDGLKAGDRVIVEGIQKTKPGAPVKPV 376 G V FVV V R + T A G+ W+V+ GLK GD+++V+G Q+ GA VK V Sbjct: 318 GAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAGAKVKVV 377 Query: 377 PW 378 PW Sbjct: 378 PW 379 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 338 bits (868), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 180/368 (48%), Positives = 251/368 (68%), Gaps = 13/368 (3%) Query: 9 RLTLTVVAAAALAACGQ-----KPNAQAGAGMPPGEVSVVTIAPERVTISNELPGRLEAT 63 RL L+++ A LAAC + KP AQA EV V + +R ++ ELPGR A Sbjct: 20 RLALSLILVAVLAACSRSGDQPKPTAQA-------EVGYVVVKAQRQALTTELPGRTTAY 72 Query: 64 RVAEVRARVAGIILKRTFREGSEVKAGEVLYRIDPATFKANYDSALATLAKSQASLTQAS 123 A++R +V GII KR F+EGS VKAG+ LY+IDPA+++A YDSA A+L K++A+L + Sbjct: 73 LAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSK 132 Query: 124 LKAQRYKPLVEVNAVSKQEYDDAVAAQKQAEADVAAGKAAVQTAGLSLGYATVTSPISGR 183 LKA+RY L +++AVSKQ+ DDA + +Q +ADV KAAVQTA ++L Y + SPISGR Sbjct: 133 LKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGR 192 Query: 184 IGRALVTEGALVGQGDATQLAVVQQLDPIYVNLTQSSTDLLKLQQAMASGQLKTVGQGKA 243 I ++ VT GALV T L VQQ+DPIYV++TQS+ DLL+L++ +ASGQ++ G+ A Sbjct: 193 IEKSAVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGENAA 252 Query: 244 KVTLVTDDGRPYPLPGKLLFSDVTVDATTGAVTIRAEFPNPNRTLLPGMYVRAQLEQAVN 303 +V +V +DG Y PGKL F+ ++VD TG +T+RA PNP+ LLPG YVRA LE+ V+ Sbjct: 253 QVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVD 312 Query: 304 ESAIVVPQQAVMRDLNG-SSVFVVGDDNKVAARPVKTGSAQGNSWIVTDGLKAGDRVIVE 362 + A++ PQ+AV RD G ++ V+G D KV R V A GN+W V+ GLK GD++IV+ Sbjct: 313 DHALLAPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLKEGDKLIVD 372 Query: 363 GIQKTKPG 370 G+QK + G Sbjct: 373 GLQKVRTG 380 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 183 bits (465), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 115/315 (36%), Positives = 172/315 (54%), Gaps = 9/315 (2%) Query: 63 TRVAEVRARVAGIILKRTFREGSEVKAGEVLYRIDPATFKANYDSALATLAKSQASLTQA 122 T A VRARV G ++K ++EG VKAG++L IDP + +A A LA+ +A L A Sbjct: 57 TATATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASA 116 Query: 123 SLKAQRYKPLVEVNAVSKQEYDDAVAAQKQAEADVAAGKAAVQTAGLSLGYATVTSPISG 182 L +RY+ L+ ++++ Q+ D VA KQ E V A + V +A L L + VT+PISG Sbjct: 117 RLDLKRYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISG 176 Query: 183 RIGRALVTEGALVGQGDATQLAVVQQLDPIYVNLTQSSTDLLKLQQAMASG-QLKTVGQG 241 R+G G V D L ++ QL PI + ++ K+ Q + SG +L Sbjct: 177 RLGLRQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPAQAWD 236 Query: 242 KAKVTLVTDDGRPYPLPGKLLFSDVTVDATTGAVTIRAEFPNPNRTLLPGMYVRAQLEQA 301 + + + D G LL D VDATTG V ++A+FPN + L P +V +L+ Sbjct: 237 REQKNKLAD--------GVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLN 288 Query: 302 VNESAIVVPQQAVMRDLNGSSVFVVGDDNKVAARPVKTGSAQGNSWIVTDGLKAGDRVIV 361 + A V+P A+ R G V+VV DD+ V RPVKTG Q + ++TDG+ AG+ V++ Sbjct: 289 TEQGATVIPTAAIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVI 348 Query: 362 EGIQKTKPGAPVKPV 376 +GI + + GA V+ V Sbjct: 349 DGIDRLREGAKVEAV 363 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 154 bits (388), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 105/315 (33%), Positives = 164/315 (52%), Gaps = 11/315 (3%) Query: 68 VRARVAGIILKRTFREGSEVKAGEVLYRIDPATFKANYDSALATLAKSQASLTQASLKAQ 127 V+ R+ G + K F+EG +VK G++L +ID +A A A AK A+L A L Q Sbjct: 79 VKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQ 138 Query: 128 RYKPLVEVNAVSKQEYDDAVAAQKQAEADVAAGKAAVQTAGLSLGYATVTSPISGRIGRA 187 RY L +A ++Q+ D A Q EA V +A V A + L Y T+ +PISGR+G Sbjct: 139 RYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGAR 198 Query: 188 LVTEGALVGQGDATQLAVVQQLDPIYVNLTQSSTDLLKLQQAMASGQLKTVGQGKAKVTL 247 LV G +V DA L V+ Q+DPI + T + + +A + Q +V+ Sbjct: 199 LVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRA------QRAHQQPLQVSA 252 Query: 248 VTDDGRPYPLPGKLLFSDVTVDATTGAVTIRAEFPNPNRTLLPGMYVRAQLEQAVNESAI 307 D + GKL+ D ++ T G V ++A F NP+ L PG YV A+L+ + +A+ Sbjct: 253 YARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNARLQMRSHGNAL 312 Query: 308 VVPQQAVMRDLNGSSVFVVGDDNKVAARPVKTGSAQGNSWIVTDG--LKAGDRVIVEGIQ 365 VP A+ R+ G+ V+ + +++ +PVK Q ++ G ++AG RV+++G Sbjct: 313 TVPAAAIQRNQQGTYVYAIDQEDQARMQPVKVERIQDGIAVLAAGNAVQAGQRVVIDGQY 372 Query: 366 KTKPGAPV---KPVP 377 K KPG+ + KP P Sbjct: 373 KLKPGSRIVESKPQP 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 970,463 Number of extensions: 33841 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 11 Length of query: 410 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 323 Effective length of database: 1,207,925 Effective search space: 390159775 Effective search space used: 390159775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)